FastQCFastQC Report
Wed 25 May 2016
SRR1294559_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294559_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802977
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC171190.6107435059224532No Hit
ACCATGTACTCTGCGTTGATACCAC134370.4793831701080673No Hit
GTACATGGTAAGCAGTGGTATCAAC128510.4584768266025729No Hit
GTGGTAAGCAGTGGTATCAACGCAG98100.349985033769453No Hit
CATGTACTCTGCGTTGATACCACTG87720.31295297820852613No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA85140.30374847884945183No Hit
GGTAAGCAGTGGTATCAACGCAGAG75800.2704267641154387No Hit
CCATGTACTCTGCGTTGATACCACT61390.2190171378502214No Hit
ACTCTGCGTTGATACCACTGCTTAC55480.19793241257420238No Hit
GTGGTATCAACGCAGAGTACATGGG55300.19729023820031344No Hit
GTATCAACGCAGAGTACATGGGAAG55010.19625562393127022No Hit
GAGTACATGGTAAGCAGTGGTATCA47960.1711037942872881No Hit
GCTTACCATGTACTCTGCGTTGATA46280.16511016679765833No Hit
GCAGTGGTATCAACGCAGAGTACAT45660.16289823284315214No Hit
GTACATGGGAAGCAGTGGTAAGCAG44730.1595803319113928No Hit
GTACTCTGCGTTGATACCACTGCTT44250.1578678669143557No Hit
GGTATCAACGCAGAGTACATGGGAA41680.14869904390938635No Hit
GCGTTGATACCACTGCTTACCATGT40890.14588061193509613No Hit
GCTTACCACTGCTTCCCATGTACTC40420.14420382329216402No Hit
GCGTTGATACCACTGCTTACCACTG36920.1317170993554353No Hit
CTGCTTACCATGTACTCTGCGTTGA35070.12511697384602155No Hit
GTATCAACGCAGAGTACATGGTAAG33730.12033634239595972No Hit
GTACATGATAAGCAGTGGTATCAAC33650.12005093156312022No Hit
GTACATGGGAAGCAGTGGTATCAAC32980.11766061583808927No Hit
TATCAACGCAGAGTACATGGGAAGC32410.11562706365410774No Hit
AAGCAGTGGTATCAACGCAGAGTAC30960.11045399230889157No Hit
ATCATGTACTCTGCGTTGATACCAC30400.108456116479015No Hit
ATGGTAAGCAGTGGTATCAACGCAG28160.10046461315950862No Hit
GTATCAACGCAGAGTACTTTTTTTT28150.10042893680540368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC751.4842368E-512.6628018
GAACCGA658.0463244E-411.6889496
GTCTACG752.0421982E-411.4195431
GCGAAAA1355.5517376E-810.573651
GTCTCGT1409.74087E-810.1754669
TAGTCCG750.00265463510.1306035
TACGACC951.6493726E-49.9973064
GTACGAC951.6493726E-49.9973063
TCGTGGG3200.09.7944112
GTATTAG2650.09.6958391
GAACAAA6450.09.5900561
CCCCTAT800.00445397669.5162861
GCGTTAA900.00109678169.5162861
GTTCCGG1106.7129666E-59.5162861
CCGTCCT1002.765073E-49.4971019
CCCTTAA2054.0927262E-109.2841821
GTCCTAA1951.6334525E-99.2722791
GTGTAGG1951.6334525E-99.2722791
TTAGACA2304.1836756E-119.0845084
GTAATAC2106.712071E-109.045183
Produced by FastQC (version 0.11.5)