FastQCFastQC Report
Wed 25 May 2016
SRR1294559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802977
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC182220.650094524500201No Hit
ACCATGTACTCTGCGTTGATACCAC143510.5119913577599816No Hit
GTACATGGTAAGCAGTGGTATCAAC130910.4670391515877583No Hit
GTGGTAAGCAGTGGTATCAACGCAG107040.38187969433926855No Hit
GTATCAACGCAGAGTACTTTTTTTT99660.35555054500982347No Hit
CATGTACTCTGCGTTGATACCACTG90620.3232991208989585No Hit
GGTAAGCAGTGGTATCAACGCAGAG82300.29361639428364916No Hit
CCATGTACTCTGCGTTGATACCACT67610.24120783010349356No Hit
GGTATCAACGCAGAGTACTTTTTTT65610.2340725592825057No Hit
TATCAACGCAGAGTACTTTTTTTTT61030.2177327891024436No Hit
ACTCTGCGTTGATACCACTGCTTAC59910.2137370374426904No Hit
GTATCAACGCAGAGTACATGGGAAG57840.206352032142968No Hit
GTGGTATCAACGCAGAGTACATGGG57210.20410442183435681No Hit
GAGTACATGGTAAGCAGTGGTATCA51030.18205643499750443No Hit
GCAGTGGTATCAACGCAGAGTACAT50040.17852447594111548No Hit
GTACATGGGAAGCAGTGGTAAGCAG48290.17228111397275112No Hit
GCTTACCATGTACTCTGCGTTGATA45350.161792265865899No Hit
GTACTCTGCGTTGATACCACTGCTT45150.16107873878380022No Hit
GGTATCAACGCAGAGTACATGGGAA44240.15783219056025077No Hit
GCGTTGATACCACTGCTTACCATGT42750.15251641379861483No Hit
GCTTACCACTGCTTCCCATGTACTC41370.14759307693213322No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA40060.1429194745443862No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38880.1387096647600034No Hit
CTGCTTACCATGTACTCTGCGTTGA37480.1337149751853119No Hit
GCGTTGATACCACTGCTTACCACTG36740.1310749249815464No Hit
GTATCAACGCAGAGTACATGGTAAG36030.12854190384009573No Hit
TATCAACGCAGAGTACATGGGAAGC35520.12672240978074384No Hit
AAGCAGTGGTATCAACGCAGAGTAC35330.12604455905275No Hit
GTACATGGGAAGCAGTGGTATCAAC34900.1245104758262376No Hit
GTACATGATAAGCAGTGGTATCAAC34090.12162069114373752No Hit
ATCATGTACTCTGCGTTGATACCAC31440.11216645730592865No Hit
CAGTGGTATCAACGCAGAGTACATG31040.11073940314173109No Hit
ATGGTAAGCAGTGGTATCAACGCAG29890.1066366224196631No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29470.10513821554725565No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG402.749138E-416.6352631
GTCGTAC951.0330223E-612.0074081
ACGATTT802.8716506E-511.8736415
ACGTTAC600.005882668311.08206416
CACGATT951.3634324E-510.99834814
GGACCGT1355.6832505E-810.5567956
GTCTAAT1451.4311809E-810.4892291
CAGGACG1303.837522E-710.2321524
GTCTTAG1502.4238034E-810.1395891
TCGTACA750.002645345910.1348852
CGTTACA750.002651225310.13217317
AAACCGT856.5893517E-410.0600046
TGGACCG1252.5684822E-69.8809835
GGACAGT3900.09.7447336
CTAGTAC1002.7528702E-49.5012853
TAGGCCC1901.0495569E-99.5012854
AGGACCG1106.832513E-59.5009455
AGGGCCG1002.7538568E-49.5009455
CGCACGA900.00111508759.49908112
GCTACAC2750.09.3285343
Produced by FastQC (version 0.11.5)