FastQCFastQC Report
Wed 25 May 2016
SRR1294558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962334
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC882732.979846296872669No Hit
ACCATGTACTCTGCGTTGATACCAC646472.1822994976258587No Hit
GTACATGGTAAGCAGTGGTATCAAC639602.1591083247196297No Hit
CATGTACTCTGCGTTGATACCACTG457261.5435801634792026No Hit
GTGGTAAGCAGTGGTATCAACGCAG449591.5176884173087843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA445491.5038479793298123No Hit
GGTAAGCAGTGGTATCAACGCAGAG388071.3100143332925998No Hit
ACTCTGCGTTGATACCACTGCTTAC307101.0366825617908042No Hit
GTATCAACGCAGAGTACATGGGAAG301551.0179473347704884No Hit
CCATGTACTCTGCGTTGATACCACT290360.9801730662376357No Hit
GTACTCTGCGTTGATACCACTGCTT266850.9008099694362621No Hit
GTACATGGGAAGCAGTGGTAAGCAG254600.859457441328358No Hit
GGTATCAACGCAGAGTACATGGGAA233500.7882298214853558No Hit
GCAGTGGTATCAACGCAGAGTACAT230920.7795204727083441No Hit
GAGTACATGGTAAGCAGTGGTATCA228640.7718238389054036No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA221600.7480587941805347No Hit
GCTTACCATGTACTCTGCGTTGATA215000.7257790647509701No Hit
GCGTTGATACCACTGCTTACCATGT207990.7021152915235082No Hit
GCTTACCACTGCTTCCCATGTACTC201880.6814896632182597No Hit
TATCAACGCAGAGTACATGGGAAGC193990.6548552594001892No Hit
GTACATGGGAAGCAGTGGTATCAAC185540.6263304542971859No Hit
GTGGTATCAACGCAGAGTACATGGG184680.6234273380381821No Hit
GCGTTGATACCACTGCTTACCACTG179050.6044220536914473No Hit
CTGCTTACCATGTACTCTGCGTTGA179050.6044220536914473No Hit
AAGCAGTGGTATCAACGCAGAGTAC175320.5918306308471631No Hit
GTATCAACGCAGAGTACATGGTAAG164160.5541576338117173No Hit
CAGTGGTATCAACGCAGAGTACATG141400.47732632444552164No Hit
ATGGTAAGCAGTGGTATCAACGCAG140290.4735792790414585No Hit
TATCAACGCAGAGTACATGGTAAGC136330.4602114413837197No Hit
ACGCAGAGTACATGGTAAGCAGTGG134170.4529198935704077No Hit
GTACATGATAAGCAGTGGTATCAAC133520.45072567779325357No Hit
GAGTAAGCAGTGGTATCAACGCAGA131810.44495320244104813No Hit
ATCATGTACTCTGCGTTGATACCAC128410.4334757660682421No Hit
ACGCAGAGTACATGGGAAGCAGTGG124280.41953405659186305No Hit
GGTATCAACGCAGAGTACATGGTAA109320.3690333365515165No Hit
GTACATAAGCAGTGGTATCAACGCA106220.3585686151527816No Hit
TACCACTGCTTACCATGTACTCTGC101610.3430065617178886No Hit
GAGTACATGGGAAGCAGTGGTAAGC101180.34155500358838675No Hit
GTGGTATCAACGCAGAGTACATGGT99080.33446599876988886No Hit
GTGATAAGCAGTGGTATCAACGCAG98630.3329469263087822No Hit
GTAAGCAGTGGTATCAACGCAGAGT86320.29139185520606387No Hit
AAAAAGTACTCTGCGTTGATACCAC83000.2801844761596768No Hit
GCTTCCCATGTACTCTGCGTTGATA79190.2673229959889735No Hit
GAGTACATGGGAAGCAGTGGTATCA79160.26722172449156645No Hit
GCTTACTCTGCGTTGATACCACTGC76660.25878243304097376No Hit
GCAGAGTACATGGTAAGCAGTGGTA73960.24966799827433367No Hit
AAAGTACTCTGCGTTGATACCACTG68640.23170918606747248No Hit
TGGTAAGCAGTGGTATCAACGCAGA68360.23076398542500612No Hit
CTGCTTACCACTGCTTCCCATGTAC68140.23002132777735396No Hit
GATAAGCAGTGGTATCAACGCAGAG65950.22262850846663473No Hit
ACTCTGCGTTGATACCACTGCTTAT64640.21820631974652419No Hit
CTTCCCATGTACTCTGCGTTGATAC64410.21742990493306968No Hit
GTATCAACGCAGAGTACTTTTTTTT64270.2169573046118365No Hit
GTACATGGTAAGCAGTGGTAAGCAG61510.20764032685038217No Hit
GTACATGGGAAGTAAGCAGTGGTAT61270.20683015487112524No Hit
GCTTATGTACTCTGCGTTGATACCA59980.20247548048261946No Hit
GCGTTGATACCACTGCTTCCCATGT58880.19876219224435865No Hit
GTTGATACCACTGCTTACCATGTAC58310.19683803379362355No Hit
GATACCACTGCTTACCATGTACTCT57240.19322601705276987No Hit
CTGCTTCCCATGTACTCTGCGTTGA56240.18985030047253282No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT55700.18802741351920477No Hit
GCGTTGATACCACTGCTTACTCTGC53070.1791492789131813No Hit
ATACCACTGCTTACCATGTACTCTG52650.17773147794948174No Hit
GAACAAAAAAAAAAAAAAAAAAAAA50680.17108131628641468No Hit
GAGTACATGATAAGCAGTGGTATCA49080.16568016975803537No Hit
CCACTGCTTACCATGTACTCTGCGT48410.16341843964927655No Hit
GCTTATCATGTACTCTGCGTTGATA48090.1623382103436007No Hit
TATCAACGCAGAGTACTTTTTTTTT46920.15838862194472333No Hit
GCTTACCACTGCTTACCATGTACTC45890.15491163386707915No Hit
CTGCTTACTCTGCGTTGATACCACT44940.1517047031158539No Hit
CCACTGCTTCCCATGTACTCTGCGT44660.15075950247338754No Hit
ATCAACGCAGAGTACATGGGAAGCA44540.1503544164837591No Hit
GTTGATACCACTGCTTACCACTGCT44440.15001684482573538No Hit
CATGGTAAGCAGTGGTATCAACGCA44400.1498818161625259No Hit
TGATACCACTGCTTACCATGTACTC43850.1480251720433955No Hit
TACCACTGCTTACCACTGCTTCCCA42940.14495326995537977No Hit
GCAGTGGTAAGCAGTGGTATCAACG41720.14083489572749056No Hit
GTAGTAAGCAGTGGTATCAACGCAG41310.13945085192959336No Hit
GGTATCAACGCAGAGTACTTTTTTT40880.1379992938000914No Hit
TACCACTGCTTCCCATGTACTCTGC40470.1366152500021942No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT40140.13550126353071598No Hit
GCGTTGATACCACTGCTTATCATGT39720.13408346256701642No Hit
ACATGGTAAGCAGTGGTATCAACGC39490.1333070477535619No Hit
AAGCAGTGGTAAGCAGTGGTATCAA38730.1307415031525817No Hit
GTATCAACGCAGAGTACATGGGAAA38480.12989757400752244No Hit
TTCCCATGTACTCTGCGTTGATACC38320.1293574593546845No Hit
ACTCTGCGTTGATACCACTGCTTCC38150.12878358753604421No Hit
GTACATGGGAAGCAGTGATAAGCAG37400.1262518001008664No Hit
CTTATGTACTCTGCGTTGATACCAC36920.12463145614235263No Hit
GCCATGTACTCTGCGTTGATACCAC36160.12206591154137245No Hit
GCAGAGTACATGGGAAGCAGTGGTA35980.12145828255692977No Hit
GTATCAACGCAGAGTAAGCAGTGGT35400.11950036694039227No Hit
GCTTATCACTGCTTCCCATGTACTC35010.11818383747409981No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33600.11342407709596554No Hit
GCTTACTTCCCATGTACTCTGCGTT33330.11251263361930154No Hit
GTACATGGCAAGCAGTGGTATCAAC33230.11217506196127783No Hit
GCGTTGATACCACTGCTTATCACTG33220.11214130479547546No Hit
GTGCTAAGCAGTGGTATCAACGCAG31450.10616628644845585No Hit
TGCTTACTCTGCGTTGATACCACTG31140.10511981430858235No Hit
TCCATGTACTCTGCGTTGATACCAC30400.10262178403920691No Hit
CTCTGCGTTGATACCACTGCTTACC30280.10221669804957847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA3400.016.229091
GAACAAA14450.014.9452491
ACGGTTC400.005290257714.2460758
GCAAAAA14200.013.5334391
CTGTCGC500.001503223713.2963379
CTACAAA3100.012.88941
TCTGTCA905.428592E-712.6631788
CGCAAAA1551.8189894E-1212.2598632
GCTAAAC550.003036847812.1082261
TGCCATA550.003073968712.08859914
CTGCTAA4800.011.8920071
TTAGTAC1201.0055373E-811.8727313
AACGATG1950.011.68948212
TATGGTA2150.011.4861774
TACAAAA7400.011.4273512
ATAAAGG752.0787105E-411.3978213
TACAGGG2450.011.2465482
GCTTATA4100.011.1378791
GTACTGT600.005815505511.0992061
GTTCAGG600.005815505511.0992061
Produced by FastQC (version 0.11.5)