FastQCFastQC Report
Wed 25 May 2016
SRR1294558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962334
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC946733.195892158007841No Hit
ACCATGTACTCTGCGTTGATACCAC685632.314492558907942No Hit
GTACATGGTAAGCAGTGGTATCAAC649332.1919540470453365No Hit
GTGGTAAGCAGTGGTATCAACGCAG494521.6693593632588357No Hit
CATGTACTCTGCGTTGATACCACTG487781.646607033508038No Hit
GGTAAGCAGTGGTATCAACGCAGAG430991.4549000889163748No Hit
ACTCTGCGTTGATACCACTGCTTAC317661.0723301288781077No Hit
GTATCAACGCAGAGTACATGGGAAG314161.0605151208472778No Hit
CCATGTACTCTGCGTTGATACCACT313721.0590298055519736No Hit
GTACTCTGCGTTGATACCACTGCTT273110.9219419552285462No Hit
GTACATGGGAAGCAGTGGTAAGCAG253540.8558791817533067No Hit
GCAGTGGTATCAACGCAGAGTACAT246990.8337682381527539No Hit
GGTATCAACGCAGAGTACATGGGAA245000.827050562158082No Hit
GAGTACATGGTAAGCAGTGGTATCA241980.8168558980857662No Hit
GCTTACCATGTACTCTGCGTTGATA222460.7509619104395386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA216900.7321929262534204No Hit
GCGTTGATACCACTGCTTACCATGT213200.7197027749065433No Hit
GCTTACCACTGCTTCCCATGTACTC207520.7005287047307968No Hit
TATCAACGCAGAGTACATGGGAAGC203140.6857430661093584No Hit
AAGCAGTGGTATCAACGCAGAGTAC201150.6790253901146865No Hit
CTGCTTACCATGTACTCTGCGTTGA190000.6413861502450433No Hit
GTGGTATCAACGCAGAGTACATGGG189070.6382467338254227No Hit
GTATCAACGCAGAGTACTTTTTTTT188240.635444889063826No Hit
GTACATGGGAAGCAGTGGTATCAAC186880.6308539145147036No Hit
GCGTTGATACCACTGCTTACCACTG184800.6238324240278105No Hit
GTATCAACGCAGAGTACATGGTAAG168050.5672891713088396No Hit
GTACTTTTTTTTTTTTTTTTTTTTT167680.5660401561741518No Hit
CAGTGGTATCAACGCAGAGTACATG154690.5221895977968724No Hit
ATGGTAAGCAGTGGTATCAACGCAG152850.5159782792892361No Hit
TATCAACGCAGAGTACATGGTAAGC147100.49656790895287295No Hit
ACGCAGAGTACATGGTAAGCAGTGG146920.4959602799684303No Hit
ACGCAGAGTACATGGGAAGCAGTGG138490.46750298919703176No Hit
GAGTAAGCAGTGGTATCAACGCAGA136780.46173051384482644No Hit
TATCAACGCAGAGTACTTTTTTTTT134490.4540001228760835No Hit
GTACATGATAAGCAGTGGTATCAAC133700.45133330677769623No Hit
ATCATGTACTCTGCGTTGATACCAC132820.44836267618708764No Hit
GGTATCAACGCAGAGTACTTTTTTT116240.39239329528675704No Hit
GGTATCAACGCAGAGTACATGGTAA114350.38601319095010894No Hit
GTGATAAGCAGTGGTATCAACGCAG112460.37963308661346085No Hit
GAGTACATGGGAAGCAGTGGTAAGC111020.37477205473791947No Hit
TACCACTGCTTACCATGTACTCTGC105100.35478781258291603No Hit
GTACATAAGCAGTGGTATCAACGCA101910.3440192766919598No Hit
GTGGTATCAACGCAGAGTACATGGT100910.34064356011172275No Hit
GTAAGCAGTGGTATCAACGCAGAGT93090.3142454564542688No Hit
GAGTACTTTTTTTTTTTTTTTTTTT92090.31086973987403177No Hit
AAAAAGTACTCTGCGTTGATACCAC89910.30351067772911494No Hit
GCTTCCCATGTACTCTGCGTTGATA83740.28268250642905224No Hit
GAGTACATGGGAAGCAGTGGTATCA81790.27609985909758994No Hit
GCAGAGTACATGGTAAGCAGTGGTA80100.2703948980769893No Hit
ACGCAGAGTACTTTTTTTTTTTTTT77900.26296832160046774No Hit
TGGTAAGCAGTGGTATCAACGCAGA77110.2603015055020805No Hit
GCTTACTCTGCGTTGATACCACTGC77080.26020023400467335No Hit
GATAAGCAGTGGTATCAACGCAGAG73720.2488578262950768No Hit
AAAGTACTCTGCGTTGATACCACTG73400.2477775969894009No Hit
CTTCCCATGTACTCTGCGTTGATAC71010.23970963436263432No Hit
ACTCTGCGTTGATACCACTGCTTAT70460.23785299024350393No Hit
CTGCTTACCACTGCTTCCCATGTAC69670.23518617414511667No Hit
GTGGTATCAACGCAGAGTACTTTTT68560.2314391287410535No Hit
GCTTATGTACTCTGCGTTGATACCA63210.2133790450367852No Hit
GTACATGGTAAGCAGTGGTAAGCAG62390.2106109574409908No Hit
GATACCACTGCTTACCATGTACTCT61070.20615501155507787No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT60980.2058511970628565No Hit
GCGTTGATACCACTGCTTCCCATGT60870.20547986823903044No Hit
GTACATGGGAAGTAAGCAGTGGTAT60720.20497351075199488No Hit
GTTGATACCACTGCTTACCATGTAC59450.2006863506950938No Hit
CTGCTTCCCATGTACTCTGCGTTGA56770.19163943026005847No Hit
GCGTTGATACCACTGCTTACTCTGC55120.18606949790266727No Hit
ATACCACTGCTTACCATGTACTCTG54280.18323389597526815No Hit
CCACTGCTTACCATGTACTCTGCGT51240.17297171757134747No Hit
GAGTACATGATAAGCAGTGGTATCA51150.17266790307912613No Hit
GCAGTGGTATCAACGCAGAGTACTT50850.171655188105055No Hit
GCTTATCATGTACTCTGCGTTGATA50770.17138513077863604No Hit
CATGGTAAGCAGTGGTATCAACGCA50480.17040617297036728No Hit
ATCAACGCAGAGTACATGGGAAGCA49050.16557889826062827No Hit
CTGCTTACTCTGCGTTGATACCACT48110.16240572467520542No Hit
GCTTACCACTGCTTACCATGTACTC48080.16230445317779832No Hit
GTAGTAAGCAGTGGTATCAACGCAG46720.1577134786286759No Hit
GCAGTGGTAAGCAGTGGTATCAACG46610.1573421498048498No Hit
CCACTGCTTCCCATGTACTCTGCGT46450.15680203515201188No Hit
GTTGATACCACTGCTTACCACTGCT46030.15538423418831232No Hit
TGATACCACTGCTTACCATGTACTC45680.15420273338522936No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT45630.1540339475562175No Hit
TACCACTGCTTACCACTGCTTCCCA45460.1534600757375772No Hit
ACATGGTAAGCAGTGGTATCAACGC45130.15234608926609897No Hit
AAGCAGTGGTAAGCAGTGGTATCAA44780.151164588463016No Hit
TACCACTGCTTCCCATGTACTCTGC43260.14603349926105563No Hit
GTATCAACGCAGAGTACATGGGAAA42330.14289408284143518No Hit
GCGTTGATACCACTGCTTATCATGT40700.13739166481564874No Hit
ACTCTGCGTTGATACCACTGCTTCC40480.1366490071679966No Hit
CTTATGTACTCTGCGTTGATACCAC39660.1338809195722022No Hit
GCTAAGCAGTGGTATCAACGCAGAG39630.1337796480747951No Hit
GCAGAGTACATGGGAAGCAGTGGTA39160.13219306128208366No Hit
GCCATGTACTCTGCGTTGATACCAC38750.13080901748418644No Hit
TTCCCATGTACTCTGCGTTGATACC37490.12655561459308776No Hit
GTATCAACGCAGAGTAAGCAGTGGT37180.12550914245321426No Hit
GCTTATCACTGCTTCCCATGTACTC37120.12530659945840003No Hit
GTACATGGGAAGCAGTGATAAGCAG36980.12483399913716683No Hit
GTGCTAAGCAGTGGTATCAACGCAG36580.12348371250507201No Hit
GCGTTGATACCACTGCTTATCACTG35350.11933158111138041No Hit
GTACATGGCAAGCAGTGGTATCAAC35140.11862268062953064No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA34540.1165972506813884No Hit
GCTTACTTCCCATGTACTCTGCGTT33380.11268141944831339No Hit
GCAGAGTACTTTTTTTTTTTTTTTT32560.10991333185251899No Hit
CTCTGCGTTGATACCACTGCTTACC32160.10856304522042418No Hit
TGCTTACTCTGCGTTGATACCACTG31740.1071452442567246No Hit
TCCATGTACTCTGCGTTGATACCAC31530.1064363437748748No Hit
ATCAACGCAGAGTACATGGTAAGCA31340.10579495762462977No Hit
GCTTACCACTGCTTACCACTGCTTC30620.10336444168685908No Hit
GAAGTAAGCAGTGGTATCAACGCAG30480.1028918413656259No Hit
GTATCAACGCAGAGTACATGATAAG30140.10174409772834528No Hit
ATGATAAGCAGTGGTATCAACGCAG30110.10164282623093816No Hit
CAGTGGTATCAACGCAGAGTACTTT30050.10144028323612396No Hit
GTACATGGGTAAGCAGTGGTATCAA29970.10117022590970498No Hit
CTGCTAAGCAGTGGTATCAACGCAG29890.10090016858328602No Hit
TATCAACGCAGAGTAAGCAGTGGTA29870.10083265425168127No Hit
TATGTACTCTGCGTTGATACCACTG29660.1001237537698315No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG250.00600404319.016021
GTAAGAC456.748545E-414.7824933
CCCATAA400.00525115414.2620141
GGGTGTC400.005279476814.2504567
AACGTTG801.292974E-714.247087512
TAGAAGT759.646446E-713.9377794
ACGATGA1101.8735591E-1013.81512413
CAAGAAC1450.013.7630123
GAACAAA4450.013.6744411
CAACGTT852.7085298E-713.40902411
TTTACAC751.4748079E-512.6707093
CTGCTAA5700.012.3437331
AACGATG853.955749E-612.29160612
ACACTAC701.09311164E-412.2124088
ACAACGT701.0936285E-412.21178910
CAATAGA550.003050260212.1011031
GTACTGT550.003062248212.0947676
GGGTGTA550.003068839412.0912957
AAACTGT550.003074276712.08843710
TGTCCCG550.003074276712.08843711
Produced by FastQC (version 0.11.5)