FastQCFastQC Report
Wed 25 May 2016
SRR1294557_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294557_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025670
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC362561.1982800503690092No Hit
ACCATGTACTCTGCGTTGATACCAC253500.8378309597543685No Hit
GTACATGGTAAGCAGTGGTATCAAC242890.802764346409225No Hit
GTGGTAAGCAGTGGTATCAACGCAG236420.7813806528801885No Hit
CATGTACTCTGCGTTGATACCACTG165130.5457634176893051No Hit
GGTAAGCAGTGGTATCAACGCAGAG161860.5349558940664382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA155140.5127459372634822No Hit
GTACATGGGAAGCAGTGGTAAGCAG133270.44046442606100467No Hit
GTATCAACGCAGAGTACATGGGAAG122330.4043071451942875No Hit
CCATGTACTCTGCGTTGATACCACT119430.3947224912168214No Hit
GCTTACCACTGCTTCCCATGTACTC111400.36818291485852717No Hit
ACTCTGCGTTGATACCACTGCTTAC108780.35952367574785093No Hit
GTACTCTGCGTTGATACCACTGCTT97490.3222096262976465No Hit
GCAGTGGTATCAACGCAGAGTACAT96780.3198630385997151No Hit
GTGGTATCAACGCAGAGTACATGGG96050.31745034983987014No Hit
GGTATCAACGCAGAGTACATGGGAA94890.3136164882488837No Hit
GAGTACATGGTAAGCAGTGGTATCA90330.29854544613259215No Hit
GCGTTGATACCACTGCTTACCACTG87500.28919214587182346No Hit
GCTTACCATGTACTCTGCGTTGATA86480.28582099171423186No Hit
TATCAACGCAGAGTACATGGGAAGC79390.262388165265875No Hit
GTACATGGGAAGCAGTGGTATCAAC75920.2509196310238724No Hit
GCGTTGATACCACTGCTTACCATGT75670.2500933677499529No Hit
CTGCTTACCATGTACTCTGCGTTGA70380.2326096368738164No Hit
GTATCAACGCAGAGTACATGGTAAG67820.22414870094888076No Hit
AAGCAGTGGTATCAACGCAGAGTAC64310.21254796458305103No Hit
GTACATAAGCAGTGGTATCAACGCA59000.19499813264500093No Hit
GTGATAAGCAGTGGTATCAACGCAG58590.19364306087577296No Hit
CAGTGGTATCAACGCAGAGTACATG56490.1867024493748492No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA55050.1819431729170729No Hit
TATCAACGCAGAGTACATGGTAAGC54300.17946438309531443No Hit
GAGTACATGGGAAGCAGTGGTAAGC53500.17682034061877205No Hit
ACGCAGAGTACATGGGAAGCAGTGG53110.17553136991145762No Hit
ACGCAGAGTACATGGTAAGCAGTGG52040.1719949630990822No Hit
ATGGTAAGCAGTGGTATCAACGCAG51690.1708381945155949No Hit
GGTATCAACGCAGAGTACATGGTAA43150.14261304107850492No Hit
GTACATGATAAGCAGTGGTATCAAC42730.14122491877832016No Hit
TACCACTGCTTACCATGTACTCTGC40140.1326648312605142No Hit
ATCATGTACTCTGCGTTGATACCAC38360.12678183675020738No Hit
GTGGTATCAACGCAGAGTACATGGT37230.12304712675209127No Hit
GCTTATGTACTCTGCGTTGATACCA36790.12159290338999297No Hit
GCTTCCCATGTACTCTGCGTTGATA35330.11676752587030309No Hit
GTACATGGTAAGCAGTGGTAAGCAG35260.11653617215360565No Hit
GAGTACATGGGAAGCAGTGGTATCA34630.11445398870332851No Hit
GTATCAACGCAGAGTACTTTTTTTT34220.11309891693410054No Hit
CTGCTTACCACTGCTTCCCATGTAC33920.11210740100539715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAC1251.4169927E-912.1577363
ACTGTCG803.7822823E-410.6846278
GAACAAA7450.010.3424931
TCTAGAC1701.5661499E-910.05695153
TAGACAG1901.0004442E-109.9978085
CCTATAC1356.4649066E-79.8500183
TTATACT2350.09.7004814
GTATTAG2650.09.6920221
GTATTGG3400.09.5125411
GTGTTAA3800.09.512541
GTGTCGA800.00446864039.512541
GGCTAGA900.00110079259.512541
CTAGACA1804.1982275E-99.4983884
GTAGCAC1304.2517368E-69.4982323
CTGTCGC1201.7062828E-59.4977619
TACATAA12750.09.3880392
GTCTTAG1752.6155249E-89.2407531
GTACATA17000.09.2327591
GGTCTAT1651.0451913E-79.2242821
AGGGTCG1554.2774263E-79.1915325
Produced by FastQC (version 0.11.5)