FastQCFastQC Report
Wed 25 May 2016
SRR1294557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294557_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025670
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC385581.2743623726315163No Hit
ACCATGTACTCTGCGTTGATACCAC271740.8981151282195349No Hit
GTGGTAAGCAGTGGTATCAACGCAG255980.8460274914316498No Hit
GTACATGGTAAGCAGTGGTATCAAC242240.8006160618970344No Hit
CATGTACTCTGCGTTGATACCACTG178990.591571453595402No Hit
GGTAAGCAGTGGTATCAACGCAGAG178140.5887621584640758No Hit
GTACATGGGAAGCAGTGGTAAGCAG133680.44181949783023267No Hit
CCATGTACTCTGCGTTGATACCACT129050.4265171019972436No Hit
GTATCAACGCAGAGTACATGGGAAG125950.4162714374006418No Hit
GTATCAACGCAGAGTACTTTTTTTT122430.40463765050385536No Hit
ACTCTGCGTTGATACCACTGCTTAC118620.39204539820932227No Hit
GCTTACCACTGCTTCCCATGTACTC114670.37899043848139424No Hit
GCAGTGGTATCAACGCAGAGTACAT102950.3402552162000483No Hit
GTACTCTGCGTTGATACCACTGCTT97520.3223087778905168No Hit
GTGGTATCAACGCAGAGTACATGGG96570.31916897744962275No Hit
GGTATCAACGCAGAGTACATGGGAA95720.3163596823182964No Hit
GAGTACATGGTAAGCAGTGGTATCA92390.30535385550968874No Hit
GCGTTGATACCACTGCTTACCACTG90390.2987437493183328No Hit
GCTTACCATGTACTCTGCGTTGATA89650.2962980100275311No Hit
TATCAACGCAGAGTACATGGGAAGC84280.27854987490374034No Hit
GCGTTGATACCACTGCTTACCATGT79940.2642059444684979No Hit
TATCAACGCAGAGTACTTTTTTTTT79720.26347883278744877No Hit
GTACATGGGAAGCAGTGGTATCAAC79000.26109919455856057No Hit
GGTATCAACGCAGAGTACTTTTTTT78960.2609669924347335No Hit
AAGCAGTGGTATCAACGCAGAGTAC75960.2510518331476995No Hit
CTGCTTACCATGTACTCTGCGTTGA74250.24540019235409016No Hit
GTATCAACGCAGAGTACATGGTAAG67190.22206651749860362No Hit
GTGATAAGCAGTGGTATCAACGCAG62160.20544210042734334No Hit
ACGCAGAGTACATGGGAAGCAGTGG59840.19777437724537045No Hit
CAGTGGTATCAACGCAGAGTACATG59740.19744387193580265No Hit
GTACTTTTTTTTTTTTTTTTTTTTT59290.19595659804274757No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA58920.1947337283973467No Hit
GTACATAAGCAGTGGTATCAACGCA56780.18766091477259583No Hit
GAGTACATGGGAAGCAGTGGTAAGC55780.18435586167691784No Hit
ACGCAGAGTACATGGTAAGCAGTGG55690.18405840689830683No Hit
TATCAACGCAGAGTACATGGTAAGC55100.18210842557185683No Hit
ATGGTAAGCAGTGGTATCAACGCAG54680.18072030327167207No Hit
GGTATCAACGCAGAGTACATGGTAA44810.14809942921733038No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42030.13891138161134559No Hit
GTACATGATAAGCAGTGGTATCAAC41160.13603598541810574No Hit
TACCACTGCTTACCATGTACTCTGC40350.13335889241060656No Hit
ATCATGTACTCTGCGTTGATACCAC40080.13246652807477352No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39100.1292275760410091No Hit
GCTTATGTACTCTGCGTTGATACCA39070.12912842444813877No Hit
GTGGTATCAACGCAGAGTACATGGT37280.12321237940687516No Hit
GCTTCCCATGTACTCTGCGTTGATA36520.1207005390541599No Hit
GTACATGGTAAGCAGTGGTAAGCAG36270.11987427578024042No Hit
CTGCTTACCACTGCTTCCCATGTAC35330.11676752587030309No Hit
GAGTACATGGGAAGCAGTGGTATCA35230.11643702056073531No Hit
AAAAAGTACTCTGCGTTGATACCAC31290.10341511136376406No Hit
GCTTACCACTGCTTACCATGTACTC30860.10199393853262252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC555.875045E-717.283691
TAGCGCT400.00527289614.2531494
TCCGTAC550.003071740712.0897848
CCGCCTA658.033548E-411.6912189
TAGGACC1451.2150849E-911.1403924
ATTCGTG600.005883359411.08193610
GACCGTC1053.4682107E-610.85738857
GTCTTAG1605.3842086E-1010.6942831
ACCGTCA1002.4028992E-510.4490288
ACGGTCC1106.0568964E-610.3626728
CTAGGAC1303.8334474E-710.2330293
ATAGGAC1409.667383E-810.1808213
ACGCACG856.604444E-410.05755411
ATACTTA1252.5647478E-69.8821846
GTATTAA3750.09.6327751
GTACCGT900.00111190579.5020996
TGTACCG900.00111190579.5020995
ATACCGT900.00111190579.5020996
GTCCTAC1856.6320354E-99.2491091
GTATTGG2900.09.1782341
Produced by FastQC (version 0.11.5)