FastQCFastQC Report
Wed 25 May 2016
SRR1294556_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294556_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3415777
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC148290.4341325560772849No Hit
GTACATGGTAAGCAGTGGTATCAAC141250.4135223113218457No Hit
ACCATGTACTCTGCGTTGATACCAC138380.4051201234741027No Hit
GTGGTAAGCAGTGGTATCAACGCAG85030.24893311243678962No Hit
CATGTACTCTGCGTTGATACCACTG76650.22439989495801393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA76150.2229360991657242No Hit
GGTAAGCAGTGGTATCAACGCAGAG74440.2179299175560934No Hit
CCATGTACTCTGCGTTGATACCACT60540.17723639453043918No Hit
GTGGTATCAACGCAGAGTACATGGG58150.17023945064329435No Hit
ACTCTGCGTTGATACCACTGCTTAC52370.1533179712844252No Hit
GAGTACATGGTAAGCAGTGGTATCA51130.14968775771954668No Hit
GTATCAACGCAGAGTACATGGGAAG47880.1401730850696635No Hit
GCTTACCATGTACTCTGCGTTGATA47040.13771390813861678No Hit
GCGTTGATACCACTGCTTACCATGT43310.12679399152813547No Hit
GCAGTGGTATCAACGCAGAGTACAT42840.12541802348338313No Hit
GTACTCTGCGTTGATACCACTGCTT41150.12047039370544389No Hit
GGTATCAACGCAGAGTACATGGGAA38180.11177544669924297No Hit
GTACATGGGAAGCAGTGGTAAGCAG37730.11045803048618222No Hit
CTGCTTACCATGTACTCTGCGTTGA35510.10395877716841584No Hit
GTACATGGGAAGCAGTGGTATCAAC35080.10269991278704671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGT550.00307553812.0878636
CGTTTGC550.003076549612.0873328
TAGACAG5100.010.6151275
ACCGCGT1002.4085357E-510.4469078
GTTCTAA3200.09.8091991
GTACATA12500.09.7402371
GTCCTAT2350.09.7143331
GTCCTAG2950.09.673171
GTCCTAC2002.582965E-109.51195051
GTCTTAG2651.8189894E-128.9735371
CTAGTAC1804.2417014E-88.969833
AGTCCGA850.00745664078.93853110
GTCTAGC1606.62576E-78.9174541
GTGTAGG3850.08.8942921
GTGTAAG5050.08.8527061
GTATTAG3900.08.7802621
CATAAGC9350.08.7358944
ACATAAG9350.08.7356393
TTAGACA3700.08.7276584
TCAATAC3500.08.6833983
Produced by FastQC (version 0.11.5)