FastQCFastQC Report
Wed 25 May 2016
SRR1294556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294556_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3415777
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC158860.46507719912628953No Hit
ACCATGTACTCTGCGTTGATACCAC144370.4226563970657335No Hit
GTACATGGTAAGCAGTGGTATCAAC143400.41981663322869145No Hit
GTATCAACGCAGAGTACTTTTTTTT110890.3246406308140139No Hit
GTGGTAAGCAGTGGTATCAACGCAG92680.27132918805882233No Hit
CATGTACTCTGCGTTGATACCACTG82670.24202399629718213No Hit
GGTAAGCAGTGGTATCAACGCAGAG79430.23253859956314477No Hit
GGTATCAACGCAGAGTACTTTTTTT71750.21005469619357467No Hit
TATCAACGCAGAGTACTTTTTTTTT67420.19737822463234572No Hit
CCATGTACTCTGCGTTGATACCACT63510.18593134153664012No Hit
GTGGTATCAACGCAGAGTACATGGG61220.17922715680795323No Hit
ACTCTGCGTTGATACCACTGCTTAC55080.16125174447863547No Hit
GAGTACATGGTAAGCAGTGGTATCA52730.1543719042548738No Hit
GTATCAACGCAGAGTACATGGGAAG51440.1505953111107663No Hit
GCTTACCATGTACTCTGCGTTGATA49790.14576478499621023No Hit
GCAGTGGTATCAACGCAGAGTACAT45490.13317614118251864No Hit
GCGTTGATACCACTGCTTACCATGT44810.13118537890500462No Hit
GTACTCTGCGTTGATACCACTGCTT42840.12541802348338313No Hit
GGTATCAACGCAGAGTACATGGGAA42180.12348581303756072No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38720.11335634615491584No Hit
GTACATGGGAAGCAGTGGTAAGCAG37550.1099310640009579No Hit
CTGCTTACCATGTACTCTGCGTTGA37080.10855509595620556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA35620.10428081224271958No Hit
AAGCAGTGGTATCAACGCAGAGTAC35080.10269991278704671No Hit
GTACATGGGAAGCAGTGGTATCAAC34820.10193873897505605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG751.4763918E-512.6695175
ACGGGTC701.0893481E-412.2170345
GCGACGT701.09262866E-412.21309958
CGCACGA701.09337554E-412.21220612
CGACGTA658.034284E-411.6911729
GTATTAG3250.09.9452131
TAGGACT2500.09.8825134
ATTAACG900.00111164399.5024173
CGTAGCA800.00452413889.49838312
CTAGACT2950.09.3413594
CTAGGAC2950.09.3413593
CCTATAC2850.09.3357083
TAGACTG4000.09.26458555
TATTCCG1151.10860405E-49.0890025
TAGGACA3250.09.0638444
TAAGACC3150.09.0499214
ATAGGAC2106.657501E-109.049923
TTTCCCG950.00182745218.99846719
GTAGGCC1804.2060492E-88.9745043
CTACGCT850.00742306338.9434514
Produced by FastQC (version 0.11.5)