FastQCFastQC Report
Wed 25 May 2016
SRR1294555_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294555_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199449
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC282200.8820268740023673No Hit
GTACATGGTAAGCAGTGGTATCAAC202410.632640182731464No Hit
ACCATGTACTCTGCGTTGATACCAC200300.6260452971746072No Hit
GTGGTAAGCAGTGGTATCAACGCAG148630.4645487394860802No Hit
CATGTACTCTGCGTTGATACCACTG138280.43219941933751715No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA124720.38981712163563165No Hit
GGTAAGCAGTGGTATCAACGCAGAG123580.3862540081120218No Hit
CCATGTACTCTGCGTTGATACCACT93810.2932067365349471No Hit
ACTCTGCGTTGATACCACTGCTTAC88340.27611004269797707No Hit
GTATCAACGCAGAGTACATGGGAAG87750.27426597517260004No Hit
GTGGTATCAACGCAGAGTACATGGG79530.2485740513444659No Hit
GCAGTGGTATCAACGCAGAGTACAT79450.24832400829017748No Hit
GTACTCTGCGTTGATACCACTGCTT75320.23541553561253828No Hit
GAGTACATGGTAAGCAGTGGTATCA73490.22969580074569088No Hit
GGTATCAACGCAGAGTACATGGGAA69520.21728741417662853No Hit
GCTTACCATGTACTCTGCGTTGATA68310.21350551298051634No Hit
GTACATGGGAAGCAGTGGTAAGCAG64450.20144093561110052No Hit
GCGTTGATACCACTGCTTACCATGT62740.19609626532568575No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA61420.19197055492992698No Hit
AAGCAGTGGTATCAACGCAGAGTAC55560.17365490120330093No Hit
GCTTACCACTGCTTCCCATGTACTC54450.17018555382504924No Hit
GTACATGATAAGCAGTGGTATCAAC54160.16927914775325378No Hit
CTGCTTACCATGTACTCTGCGTTGA53080.16590356652036023No Hit
GCGTTGATACCACTGCTTACCACTG52650.16455958510356003No Hit
GTACATAAGCAGTGGTATCAACGCA52220.16321560368675983No Hit
ATCATGTACTCTGCGTTGATACCAC50560.1580272103102753No Hit
GTATCAACGCAGAGTACATGGTAAG50360.15740210267455426No Hit
TATCAACGCAGAGTACATGGGAAGC49500.15471413984095386No Hit
GTACATGGGAAGCAGTGGTATCAAC47360.1480254881387389No Hit
CAGTGGTATCAACGCAGAGTACATG44730.1398053227290074No Hit
ATGGTAAGCAGTGGTATCAACGCAG44020.13758619062219776No Hit
ACGCAGAGTACATGGTAAGCAGTGG41300.12908472677639182No Hit
TATCAACGCAGAGTACATGGTAAGC41150.12861589604960103No Hit
GTGATAAGCAGTGGTATCAACGCAG37570.11742646937019467No Hit
GTATCAACGCAGAGTACTTTTTTTT35500.11095660534048207No Hit
GCTTATGTACTCTGCGTTGATACCA32310.1009861385507317No Hit
GGTATCAACGCAGAGTACATGGTAA32240.10076735087822934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC604.0767988E-412.673038519
TCGCTAA550.00304061412.1062711
TAGGCCC1406.8394E-1011.5335794
GCGATAA600.005822673411.0974151
GTCTAGT700.001476427810.8709361
GTCTTAG1408.198185E-910.8709361
CGCTCGA700.001494650510.85496314
CGACGTA700.001494845410.85479459
ACCGACC803.780578E-410.6851878
CCCATAC1803.6379788E-1110.5539323
TAGGACC1409.7259544E-810.1766874
TCTATAC2650.010.0361923
GCTAAAC1054.0509898E-59.9650251
GGCCTAA1252.5307709E-69.8925521
TACACCG1356.469454E-79.84956555
AGCACCG1554.0827217E-89.8041765
CTATACC1951.6370905E-109.7417864
TAGACAG2750.09.6704835
GTCTTAT2106.548362E-119.512071
CGTAATG800.00451057649.5018062
Produced by FastQC (version 0.11.5)