FastQCFastQC Report
Wed 25 May 2016
SRR1294555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199449
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC300120.9380365181629711No Hit
ACCATGTACTCTGCGTTGATACCAC214130.6692714901847162No Hit
GTACATGGTAAGCAGTGGTATCAAC203710.6367033823636508No Hit
GTGGTAAGCAGTGGTATCAACGCAG162690.5084938062772684No Hit
CATGTACTCTGCGTTGATACCACTG146630.45829766312887No Hit
GGTAAGCAGTGGTATCAACGCAGAG136880.42782366588747No Hit
GTATCAACGCAGAGTACTTTTTTTT131910.41228974113980255No Hit
CCATGTACTCTGCGTTGATACCACT100830.31514801454875513No Hit
GTATCAACGCAGAGTACATGGGAAG94600.2956759116960451No Hit
ACTCTGCGTTGATACCACTGCTTAC93850.29333175806209133No Hit
GTGGTATCAACGCAGAGTACATGGG85110.2660145543810825No Hit
GGTATCAACGCAGAGTACTTTTTTT84330.2635766346017705No Hit
GCAGTGGTATCAACGCAGAGTACAT82890.2590758596245791No Hit
TATCAACGCAGAGTACTTTTTTTTT81610.25507517075596453No Hit
GAGTACATGGTAAGCAGTGGTATCA76630.23950999062651102No Hit
GTACTCTGCGTTGATACCACTGCTT76220.23822851997328287No Hit
GGTATCAACGCAGAGTACATGGGAA73030.22825805318353257No Hit
GCTTACCATGTACTCTGCGTTGATA69120.2160371989051865No Hit
GTACATGGGAAGCAGTGGTAAGCAG67070.20962984563904596No Hit
AAGCAGTGGTATCAACGCAGAGTAC66510.20787954425902708No Hit
GCGTTGATACCACTGCTTACCATGT65410.20444145226256147No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA64440.2014096802293145No Hit
GTACTTTTTTTTTTTTTTTTTTTTT56020.1750926487654593No Hit
GCGTTGATACCACTGCTTACCACTG55470.1733736027672265No Hit
GCTTACCACTGCTTCCCATGTACTC55110.17224840902292862No Hit
CTGCTTACCATGTACTCTGCGTTGA54790.17124823680577497No Hit
GTACATGATAAGCAGTGGTATCAAC54580.17059187378826793No Hit
GTATCAACGCAGAGTACATGGTAAG53900.1684665078268164No Hit
GTACATAAGCAGTGGTATCAACGCA53220.16634114186536494No Hit
ATCATGTACTCTGCGTTGATACCAC52880.1652784588846392No Hit
TATCAACGCAGAGTACATGGGAAGC52810.16505967121213685No Hit
GTACATGGGAAGCAGTGGTATCAAC50090.1565582073663309No Hit
CAGTGGTATCAACGCAGAGTACATG48310.15099474940841376No Hit
ATGGTAAGCAGTGGTATCAACGCAG48140.15046340791805088No Hit
ACGCAGAGTACATGGTAAGCAGTGG45290.14155562410902628No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43780.13683606145933253No Hit
TATCAACGCAGAGTACATGGTAAGC42840.1338980555714437No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40590.12686559466958217No Hit
GTGATAAGCAGTGGTATCAACGCAG39520.1235212688184747No Hit
ACGCAGAGTACATGGGAAGCAGTGG33630.10511184894649048No Hit
GCTTATGTACTCTGCGTTGATACCA33390.10436171978362525No Hit
GGTATCAACGCAGAGTACATGGTAA33050.1032990368028995No Hit
TACCACTGCTTACCATGTACTCTGC32100.10032977553322461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG1751.8189894E-1211.4055181
GTTATAC1252.2368295E-710.6419873
TACGGGT909.503373E-510.5575274
TCTATCC1002.3956096E-510.4519513
CTTAGAC1106.037053E-610.3655723
CCCTATA1106.038179E-610.365412
GTCTAGA1951.4551915E-1110.2357211
ACCGTGC1303.848745E-710.2301228
ACGTTAC750.00265199310.131916
TAGGATA1451.6144259E-79.8294224
TCTATAC2550.09.6880843
ACGGGTG900.00111229059.5017745
TAGGACT1804.1745807E-99.5017744
CTAGGCC2002.6375346E-109.5017743
CGCCTAA1002.760373E-49.49880414
GGACTGT3450.09.3640676
TAAGGCT2451.8189894E-129.3078614
TCTAAAC1856.684786E-99.244973
CTGGTCG1752.6753696E-89.2279869
TTAGACA1651.06365405E-79.2138414
Produced by FastQC (version 0.11.5)