FastQCFastQC Report
Wed 25 May 2016
SRR1294554_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294554_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2148338
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC651803.033973238847891No Hit
GTACATGGTAAGCAGTGGTATCAAC446872.0800730611291147No Hit
ACCATGTACTCTGCGTTGATACCAC439162.0441848535938014No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA356191.6579793309991258No Hit
GTGGTAAGCAGTGGTATCAACGCAG328731.530159593136648No Hit
CATGTACTCTGCGTTGATACCACTG300311.3978712846861154No Hit
GGTAAGCAGTGGTATCAACGCAGAG279791.3023555883664488No Hit
ACTCTGCGTTGATACCACTGCTTAC206030.959020414850922No Hit
GTATCAACGCAGAGTACATGGGAAG204360.9512469639321187No Hit
CCATGTACTCTGCGTTGATACCACT203430.9469180361749409No Hit
GTACTCTGCGTTGATACCACTGCTT179830.8370656758852657No Hit
GTACATGGGAAGCAGTGGTAAGCAG173750.8087647288275867No Hit
GAGTACATGGTAAGCAGTGGTATCA163330.7602621189030777No Hit
GCAGTGGTATCAACGCAGAGTACAT162290.7554211674326852No Hit
GGTATCAACGCAGAGTACATGGGAA151200.7037998676185964No Hit
GCTTACCATGTACTCTGCGTTGATA147980.6888115371044966No Hit
GCTTACCACTGCTTCCCATGTACTC145650.6779659439064059No Hit
GCGTTGATACCACTGCTTACCATGT140440.6537146389441513No Hit
TATCAACGCAGAGTACATGGGAAGC136370.6347697615552116No Hit
GTACATGGGAAGCAGTGGTATCAAC131620.6126596466663998No Hit
GCGTTGATACCACTGCTTACCACTG127730.5945526262627202No Hit
CTGCTTACCATGTACTCTGCGTTGA123200.5734665588003378No Hit
GTGGTATCAACGCAGAGTACATGGG116820.5437691834338917No Hit
AAGCAGTGGTATCAACGCAGAGTAC116420.5419072790222023No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA109480.5096032374793911No Hit
GTATCAACGCAGAGTACATGGTAAG108350.5043433575163685No Hit
ATGGTAAGCAGTGGTATCAACGCAG102230.47585622001752054No Hit
TATCAACGCAGAGTACATGGTAAGC99700.464079674613585No Hit
GAGTAAGCAGTGGTATCAACGCAGA97620.4543977716728001No Hit
CAGTGGTATCAACGCAGAGTACATG91560.42618991983570553No Hit
ACGCAGAGTACATGGTAAGCAGTGG89800.4179975404242722No Hit
GTACATGATAAGCAGTGGTATCAAC81840.3809456426316529No Hit
GTACATAAGCAGTGGTATCAACGCA76770.35734600421348967No Hit
GTGATAAGCAGTGGTATCAACGCAG76430.3557633854635537No Hit
ACGCAGAGTACATGGGAAGCAGTGG76050.35399457627244874No Hit
GGTATCAACGCAGAGTACATGGTAA71290.3318379137733448No Hit
GAGTACATGGGAAGCAGTGGTAAGC71090.3309069615675001No Hit
TACCACTGCTTACCATGTACTCTGC69980.325740176825062No Hit
ATCATGTACTCTGCGTTGATACCAC67650.31489458362697115No Hit
GTGGTATCAACGCAGAGTACATGGT62810.29236554024552935No Hit
GTAAGCAGTGGTATCAACGCAGAGT59660.27770304300347526No Hit
GCTTACTCTGCGTTGATACCACTGC56120.26122518896002395No Hit
AAAAAGTACTCTGCGTTGATACCAC55980.2605735224159327No Hit
GTACATGGTAAGCAGTGGTAAGCAG55560.2586185227836588No Hit
GAGTACATGGGAAGCAGTGGTATCA54410.25326554760005177No Hit
GCTTCCCATGTACTCTGCGTTGATA54370.25307935715888286No Hit
GCAGAGTACATGGTAAGCAGTGGTA50530.23520507480666453No Hit
TGGTAAGCAGTGGTATCAACGCAGA48470.22561626708646407No Hit
AAAGTACTCTGCGTTGATACCACTG47130.2193788873073045No Hit
GCTTATGTACTCTGCGTTGATACCA45610.21230365054288478No Hit
GCTTACCACTGCTTACCATGTACTC45570.21211746010171584No Hit
CTGCTTACCACTGCTTCCCATGTAC45490.21174507921937796No Hit
CTTCCCATGTACTCTGCGTTGATAC41860.19484829668329656No Hit
GATAAGCAGTGGTATCAACGCAGAG40960.1906590117569954No Hit
GTTGATACCACTGCTTACCATGTAC40610.18902984539676718No Hit
GCGTTGATACCACTGCTTCCCATGT39990.18614389355864858No Hit
GTATCAACGCAGAGTACTTTTTTTT39760.18507329852192717No Hit
GCGTTGATACCACTGCTTACTCTGC39080.1819080610220552No Hit
ACTCTGCGTTGATACCACTGCTTAT38640.17985996616919686No Hit
CTGCTTCCCATGTACTCTGCGTTGA36760.17110901543425663No Hit
GATACCACTGCTTACCATGTACTCT36590.17031770605928861No Hit
ATACCACTGCTTACCATGTACTCTG34590.16100818400084158No Hit
CTGCTTACTCTGCGTTGATACCACT33570.1562603277510336No Hit
CCACTGCTTACCATGTACTCTGCGT33060.1538863996261296No Hit
GTAGTAAGCAGTGGTATCAACGCAG32340.15053497168508867No Hit
GTTGATACCACTGCTTACCACTGCT31540.14681116286170984No Hit
GAGTACATGATAAGCAGTGGTATCA31020.14439068712651362No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT30800.14336663970008445No Hit
TACCACTGCTTACCACTGCTTCCCA30620.1425287827148242No Hit
CATGGTAAGCAGTGGTATCAACGCA30310.1410858067957649No Hit
GCAGTGGTAAGCAGTGGTATCAACG29940.1393635452149522No Hit
TGATACCACTGCTTACCATGTACTC29540.1375016408032628No Hit
AAACAAAAAAAAAAAAAAAAAAAAA29160.13573283161215788No Hit
GTACATGGGAAGCAGTAAGCAGTGG29040.13517426028865104No Hit
GCAGTAAGCAGTGGTATCAACGCAG29030.1351277126783588No Hit
ATCAACGCAGAGTACATGGGAAGCA28940.13470878418572868No Hit
GTACATAGTAAGCAGTGGTATCAAC28130.13093842775205763No Hit
CCACTGCTTCCCATGTACTCTGCGT27990.13028676120796634No Hit
TATCAACGCAGAGTACTTTTTTTTT27760.12921616617124493No Hit
ACTATGTACTCTGCGTTGATACCAC27660.12875069006832257No Hit
GAACAAAAAAAAAAAAAAAAAAAAA27390.12749390459043222No Hit
GCTTATCATGTACTCTGCGTTGATA27120.12623711911254187No Hit
TACCACTGCTTCCCATGTACTCTGC27110.12619057150224966No Hit
CTTATGTACTCTGCGTTGATACCAC27040.125864738230204No Hit
AAGCAGTGGTAAGCAGTGGTATCAA26460.12316497683325436No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT26440.12307188161266988No Hit
ACATGGTAAGCAGTGGTATCAACGC26010.12107033437010377No Hit
GTACATGGGAAGCAGTGATAAGCAG26000.12102378675981154No Hit
GTACATGGGAAGTAAGCAGTGGTAT25810.12013938216425905No Hit
GTATCAACGCAGAGTAAGCAGTGGT25490.11864985863490754No Hit
GGTATCAACGCAGAGTACTTTTTTT24930.11604319245854239No Hit
GCTTATCACTGCTTCCCATGTACTC24920.11599664484825012No Hit
ACTCTGCGTTGATACCACTGCTTCC24450.11380890716451508No Hit
GCCATGTACTCTGCGTTGATACCAC24390.11352962150276166No Hit
GCGTTGATACCACTGCTTATCACTG24180.11255212168662473No Hit
GCGTTGATACCACTGCTTATCATGT24080.11208664558370238No Hit
TTCCCATGTACTCTGCGTTGATACC23890.1112022409881499No Hit
GCAGAGTACATGGGAAGCAGTGGTA23870.11110914576756543No Hit
TCCATGTACTCTGCGTTGATACCAC23300.10845593198090804No Hit
GTACATGGCAAGCAGTGGTATCAAC22950.1068267656206798No Hit
GTATCAACGCAGAGTACATGGGAAA22910.10664057517951087No Hit
TGCTTACTCTGCGTTGATACCACTG22900.10659402756921864No Hit
GTACATGGGTAAGCAGTGGTATCAA22110.10291676635613203No Hit
GTGCATGGTAAGCAGTGGTATCAAC21970.10226509981204074No Hit
ACCATGCACTCTGCGTTGATACCAC21500.10007736212830569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA7050.014.3069011
CTGCAAA1205.0931703E-1113.48020651
GTACAGG1850.013.3730511
GTACTGC500.001502589813.2968056
GTCTCAC550.003031998912.1105591
TAGCGAA1103.7847713E-812.09814518
CGCTAAG550.003066060612.092512
TAGCACG550.003073019412.0888494
AGCCTCG550.00307677212.0868810
GCTTATA1750.011.9622671
AAAACGC1209.93532E-911.88182917
ACTTAGC802.873241E-511.87270215
AACGTTG1603.6379788E-1211.8713212
GCACTGT1052.7300302E-711.7590816
GTACAAA1950.011.7113111
TTTACCC658.035516E-411.6905883
TGGAGTC658.041796E-411.68955
TAAGGTG658.041796E-411.68955
TCTGTTG658.0465083E-411.68868358
AACGATA658.0465083E-411.688683512
Produced by FastQC (version 0.11.5)