FastQCFastQC Report
Wed 25 May 2016
SRR1294554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294554_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2148338
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC684273.1851133294667786No Hit
ACCATGTACTCTGCGTTGATACCAC464822.163626021603677No Hit
GTACATGGTAAGCAGTGGTATCAAC454912.1174973398040717No Hit
GTGGTAAGCAGTGGTATCAACGCAG367221.7093213451514613No Hit
CATGTACTCTGCGTTGATACCACTG321301.4955747186895172No Hit
GGTAAGCAGTGGTATCAACGCAGAG314411.4635034151981672No Hit
CCATGTACTCTGCGTTGATACCACT218781.0183686179735219No Hit
ACTCTGCGTTGATACCACTGCTTAC217881.0141793330472206No Hit
GTATCAACGCAGAGTACATGGGAAG215911.0050094538196503No Hit
GTACATGGGAAGCAGTGGTAAGCAG184770.8600601953696299No Hit
GTACTCTGCGTTGATACCACTGCTT180520.8402774609954299No Hit
GCAGTGGTATCAACGCAGAGTACAT174520.8123488948200888No Hit
GAGTACATGGTAAGCAGTGGTATCA173670.8083923479452488No Hit
GGTATCAACGCAGAGTACATGGGAA163090.759144976256064No Hit
GCTTACCATGTACTCTGCGTTGATA153070.7125042707432443No Hit
GCGTTGATACCACTGCTTACCATGT150600.7010070110010622No Hit
GCTTACCACTGCTTCCCATGTACTC147900.6884391562221587No Hit
TATCAACGCAGAGTACATGGGAAGC144670.6734042780977667No Hit
AAGCAGTGGTATCAACGCAGAGTAC140270.6529233295691833No Hit
GCGTTGATACCACTGCTTACCACTG135780.6320234525479697No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA135220.6294167863716045No Hit
GTACATGGGAAGCAGTGGTATCAAC134180.624575834901212No Hit
GTATCAACGCAGAGTACTTTTTTTT130710.6084238141298064No Hit
CTGCTTACCATGTACTCTGCGTTGA128950.6002314347183729No Hit
GTACTTTTTTTTTTTTTTTTTTTTT126410.5884083417041452No Hit
GTGGTATCAACGCAGAGTACATGGG125590.584591437660182No Hit
GTATCAACGCAGAGTACATGGTAAG114410.532551209353463No Hit
ATGGTAAGCAGTGGTATCAACGCAG112600.5241260918905685No Hit
TATCAACGCAGAGTACATGGTAAGC103840.4833503852745704No Hit
GAGTAAGCAGTGGTATCAACGCAGA101130.47073598288537466No Hit
CAGTGGTATCAACGCAGAGTACATG100750.4689671736942697No Hit
ACGCAGAGTACATGGTAAGCAGTGG100370.4671983645031648No Hit
TATCAACGCAGAGTACTTTTTTTTT96530.44932408215094644No Hit
ACGCAGAGTACATGGGAAGCAGTGG88510.41199289869657385No Hit
GTGATAAGCAGTGGTATCAACGCAG84700.39425825917523216No Hit
GTACATGATAAGCAGTGGTATCAAC84160.3917446882194515No Hit
GGTATCAACGCAGAGTACTTTTTTT80970.37689600053622846No Hit
GAGTACATGGGAAGCAGTGGTAAGC77630.36134909869862186No Hit
GGTATCAACGCAGAGTACATGGTAA77610.36125600347803744No Hit
GTACATAAGCAGTGGTATCAACGCA76200.35469279042683227No Hit
ATCATGTACTCTGCGTTGATACCAC71970.3350031512732168No Hit
TACCACTGCTTACCATGTACTCTGC71360.3321637470453904No Hit
GAGTACTTTTTTTTTTTTTTTTTTT69480.3234127963104502No Hit
GTAAGCAGTGGTATCAACGCAGAGT66790.31089148914183895No Hit
GTGGTATCAACGCAGAGTACATGGT65960.30702803748758345No Hit
AAAAAGTACTCTGCGTTGATACCAC59770.27821506671668983No Hit
GTACATGGTAAGCAGTGGTAAGCAG58070.2703019729670098No Hit
GCTTCCCATGTACTCTGCGTTGATA57100.26578685476866304No Hit
GAGTACATGGGAAGCAGTGGTATCA56580.2633663790334668No Hit
GCAGAGTACATGGTAAGCAGTGGTA56480.26290090293054447No Hit
GCTTACTCTGCGTTGATACCACTGC56000.26066661763651716No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55660.2590839988865811No Hit
TGGTAAGCAGTGGTATCAACGCAGA53860.2507054290339788No Hit
AAAGTACTCTGCGTTGATACCACTG48820.22724543344669226No Hit
GCTTACCACTGCTTACCATGTACTC48600.22622138602026312No Hit
CTGCTTACCACTGCTTCCCATGTAC48000.22342852940272898No Hit
GATAAGCAGTGGTATCAACGCAGAG47070.21909960164555112No Hit
GCTTATGTACTCTGCGTTGATACCA46670.2172376972338617No Hit
CTTCCCATGTACTCTGCGTTGATAC45510.21183817443996245No Hit
GTGGTATCAACGCAGAGTACTTTTT44330.20634555642547867No Hit
ACTCTGCGTTGATACCACTGCTTAT42040.19568615366855682No Hit
GCGTTGATACCACTGCTTCCCATGT41760.19438282058037423No Hit
GCGTTGATACCACTGCTTACTCTGC39850.1854922270145573No Hit
GTTGATACCACTGCTTACCATGTAC39350.18316484649994552No Hit
CTGCTTCCCATGTACTCTGCGTTGA38530.17934794245598226No Hit
GATACCACTGCTTACCATGTACTCT37160.17297091984594604No Hit
ATACCACTGCTTACCATGTACTCTG37090.17264508657390037No Hit
GTAGTAAGCAGTGGTATCAACGCAG36780.1712021106548411No Hit
GCAGTGGTATCAACGCAGAGTACTT36560.17017806322841192No Hit
CCACTGCTTACCATGTACTCTGCGT35870.16696627811824769No Hit
GCAGTGGTAAGCAGTGGTATCAACG35440.16496473087568156No Hit
GCAGTAAGCAGTGGTATCAACGCAG35280.1642199691110058No Hit
CTGCTTACTCTGCGTTGATACCACT35240.16403377866983687No Hit
CATGGTAAGCAGTGGTATCAACGCA34920.16254425514048534No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT33430.1556086612069423No Hit
TACCACTGCTTACCACTGCTTCCCA32740.15239687609677807No Hit
GTTGATACCACTGCTTACCACTGCT32670.15207104282473244No Hit
GAGTACATGATAAGCAGTGGTATCA31870.1483472340013536No Hit
AAGCAGTGGTAAGCAGTGGTATCAA31650.14732318657492444No Hit
ATCAACGCAGAGTACATGGGAAGCA31560.14690425808229432No Hit
CCACTGCTTCCCATGTACTCTGCGT30400.14150473528839502No Hit
GTACATGGGAAGCAGTAAGCAGTGG29890.13913080716349105No Hit
CTTATGTACTCTGCGTTGATACCAC29610.13782747407530843No Hit
TGATACCACTGCTTACCATGTACTC29500.13731545036209386No Hit
ACATGGTAAGCAGTGGTATCAACGC29460.13712925992092492No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT29150.13568628400186564No Hit
ACTATGTACTCTGCGTTGATACCAC29060.13526735550923552No Hit
TACCACTGCTTCCCATGTACTCTGC28350.1319624751784868No Hit
GTACATAGTAAGCAGTGGTATCAAC28140.13098497536234988No Hit
GCTTATCATGTACTCTGCGTTGATA27690.1288903328991993No Hit
GCAGAGTACATGGGAAGCAGTGGTA27060.12595783345078845No Hit
GTACATGGCAAGCAGTGGTATCAAC26410.12293223878179317No Hit
GTACATGGGAAGCAGTGATAAGCAG26340.12260640550974754No Hit
ACTCTGCGTTGATACCACTGCTTCC25880.12046521543630472No Hit
TTCCCATGTACTCTGCGTTGATACC25500.11869640624519978No Hit
GTATCAACGCAGAGTAAGCAGTGGT25370.11809128731140071No Hit
GTACATGGGTAAGCAGTGGTATCAA25290.11771890642906283No Hit
GTACATGGGAAGTAAGCAGTGGTAT25200.11729997793643272No Hit
GCCATGTACTCTGCGTTGATACCAC25140.1170206922746793No Hit
GCTTATCACTGCTTCCCATGTACTC25060.11664831139234144No Hit
GTGCATGGTAAGCAGTGGTATCAAC24830.11557771635562003No Hit
GCGTTGATACCACTGCTTATCATGT24730.11511224025269767No Hit
TGCTTACTCTGCGTTGATACCACTG24300.11311069301013156No Hit
GCGTTGATACCACTGCTTATCACTG24270.11297105017925484No Hit
GTATCAACGCAGAGTACATGGGAAA24060.11199355036311792No Hit
ACCATGCACTCTGCGTTGATACCAC23560.10966616984850615No Hit
TATCAACGCAGAGTAAGCAGTGGTA23060.10733878933389439No Hit
TCCATGTACTCTGCGTTGATACCAC22920.10668712278980311No Hit
GCAGAGTACTTTTTTTTTTTTTTTT22910.10664057517951087No Hit
GAGTACATGGTAAGCAGTGGTAAGC22310.10384771856197675No Hit
GCTAAGCAGTGGTATCAACGCAGAG21960.10221855220174851No Hit
GTGCTAAGCAGTGGTATCAACGCAG21880.10184617131941062No Hit
CTCTGCGTTGATACCACTGCTTACC21540.10026355256947464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGA250.00603806518.99713710
GTGCGTT456.7715504E-414.77555111
GAACAAA3300.014.695341
GCATAAC400.00524782514.2631241
GTCATGG400.00524782514.2631241
CCCGTAC400.005286886414.247189516
TCGCTAA759.577725E-713.9461651
TGTACTG551.9577888E-413.8206035
GTACTGG1051.3533281E-913.5839281
CAAAAAC3600.013.4606913
TAGACAG500.001497893713.302335
CCTCTGT500.001500787113.2989248
AAACTCT1650.013.2447456
CCAAAAA3500.013.0314622
CACAACG1102.752131E-912.9534999
ACTGTAC604.0959503E-412.6656438
CTGCTAA4150.012.6019561
GTTCTAT1155.340553E-912.38943811
AGTTCTA1251.4151738E-912.15816810
CTAGACA550.003065077112.0930284
Produced by FastQC (version 0.11.5)