FastQCFastQC Report
Wed 25 May 2016
SRR1294553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129260
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC87770.4122089364380114No Hit
GTACATGGTAAGCAGTGGTATCAAC61070.28681325906653016No Hit
ACCATGTACTCTGCGTTGATACCAC60740.2852634248518265No Hit
GTGGTAAGCAGTGGTATCAACGCAG49820.23397800174708586No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA46740.2195128824098513No Hit
CATGTACTCTGCGTTGATACCACTG44820.21049566516066612No Hit
GGTAAGCAGTGGTATCAACGCAGAG36670.17221945652480206No Hit
GTGGTATCAACGCAGAGTACATGGG34850.16367188600734528No Hit
CCATGTACTCTGCGTTGATACCACT28260.13272216638644413No Hit
GTATCAACGCAGAGTACATGGGAAG28020.131595014230296No Hit
ACTCTGCGTTGATACCACTGCTTAC27720.1301860740351108No Hit
GCAGTGGTATCAACGCAGAGTACAT23160.10877018306829603No Hit
GGTATCAACGCAGAGTACATGGGAA23100.10848839502925899No Hit
GAGTACATGGTAAGCAGTGGTATCA22960.10783088960483925No Hit
GTACTCTGCGTTGATACCACTGCTT22890.10750213689262936No Hit
GCTTACCATGTACTCTGCGTTGATA22370.10505997388764172No Hit
GTACATGGGAAGCAGTGGTAAGCAG22360.10501300921446888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT350.002173172516.28277612
TACGGTC508.72978E-515.1972585
TAGTACG1207.385097E-1012.6691422
AGTACGG1352.7284841E-1112.6646783
ATACCGT604.0993668E-412.6643816
CGGGACT550.003072156612.0892944
CTACGAC550.00307269712.0890113
TACGTTG658.0377236E-411.6901975
TAGGACG752.070741E-411.4022272
GTGCTAG1850.011.3085391
GTACGGG855.332019E-511.1749784
ACGTTAC600.00588168211.08211416
CTGACCG803.777384E-410.68582259
GTCCTAT1201.5048936E-610.3019081
GGACCGA1054.1142986E-59.9505846
TGCGTGA1054.1164225E-59.95011710
GTATTGG2500.09.5094531
TGGACTG3000.09.4982855
AGGACCG1201.704412E-59.4982855
GTTCTAT2451.8189894E-129.3153831
Produced by FastQC (version 0.11.5)