FastQCFastQC Report
Wed 25 May 2016
SRR1294553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129260
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC92780.4357382376976039No Hit
GTATCAACGCAGAGTACTTTTTTTT69380.32584090247315967No Hit
ACCATGTACTCTGCGTTGATACCAC64000.3005739083061721No Hit
GTACATGGTAAGCAGTGGTATCAAC62690.2944215361205301No Hit
GTGGTAAGCAGTGGTATCAACGCAG55210.2592919605872463No Hit
GGTATCAACGCAGAGTACTTTTTTT48960.22993903985422168No Hit
CATGTACTCTGCGTTGATACCACTG46590.2188084123122587No Hit
TATCAACGCAGAGTACTTTTTTTTT42880.2013845185651353No Hit
GGTAAGCAGTGGTATCAACGCAGAG40700.19114621981345634No Hit
GTGGTATCAACGCAGAGTACATGGG36340.17066962231009833No Hit
CCATGTACTCTGCGTTGATACCACT30560.1435240412161972No Hit
GTATCAACGCAGAGTACATGGGAAG30070.14122277223072804No Hit
ACTCTGCGTTGATACCACTGCTTAC28830.13539915275729597No Hit
GAGTACATGGTAAGCAGTGGTATCA25600.12022956332246883No Hit
GTACATGGGAAGCAGTGGTAAGCAG24940.11712989489306143No Hit
GGTATCAACGCAGAGTACATGGGAA24730.1161436367564318No Hit
GCAGTGGTATCAACGCAGAGTACAT24360.11440594384903675No Hit
GCTTACCATGTACTCTGCGTTGATA24070.11304396832702442No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23650.11107145205376517No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA23260.10923982980002442No Hit
GTACTCTGCGTTGATACCACTGCTT22910.10759606623897504No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22680.10651587875599973No Hit
GTACATGGGAAGCAGTGGTATCAAC21460.10078618862891334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAC400.00527302814.2528183
TACCCCG655.4430257E-513.1567575
AGCACCG853.9355E-612.2968395
CCGTACC550.003064004712.0935854
ACTTCGT550.003071020112.0898928
TAGAAGT2350.010.5129644
GCACCGT750.002643402410.1355766
TGGACCG856.5816735E-410.0610495
GGACCGA951.6408027E-410.00221356
GTACTAA1554.031608E-89.8116181
GTTATAG1651.0135409E-89.79303551
GTCTTAG1752.5502231E-99.7765771
TAAGACT2151.0913936E-119.7230834
GTATTAC1701.6509148E-89.5050061
TTAGACA2600.09.5021024
GTATTAT3550.09.1033861
TTAGGCC1054.483257E-49.0496234
GCACCGC1054.483257E-49.0496236
ATAAGAC1901.0542863E-89.00178053
TTAAGAC2651.8189894E-128.9640383
Produced by FastQC (version 0.11.5)