FastQCFastQC Report
Wed 25 May 2016
SRR1294552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3077946
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC122510.39802517653006253No Hit
ACCATGTACTCTGCGTTGATACCAC91270.29652891896089145No Hit
GTACATGGTAAGCAGTGGTATCAAC90220.29311755306948206No Hit
GTGGTAAGCAGTGGTATCAACGCAG75690.2459107469721691No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA70490.2290163635099511No Hit
CATGTACTCTGCGTTGATACCACTG65780.21371395079705752No Hit
GGTAAGCAGTGGTATCAACGCAGAG54540.17719609115949403No Hit
GTGGTATCAACGCAGAGTACATGGG53940.1752467392215458No Hit
CCATGTACTCTGCGTTGATACCACT39250.12752010594078No Hit
ACTCTGCGTTGATACCACTGCTTAC38030.12355642366695192No Hit
GTATCAACGCAGAGTACATGGGAAG37680.1224193017031488No Hit
GCAGTGGTATCAACGCAGAGTACAT33260.1080590757602635No Hit
GAGTACATGGTAAGCAGTGGTATCA33240.10799409736233188No Hit
GTACATGATAAGCAGTGGTATCAAC32000.10396543669057223No Hit
GCTTACCATGTACTCTGCGTTGATA31680.1029257823236665No Hit
GTACATGGGAAGCAGTGGTAAGCAG31500.10234097674228203No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG602.572094E-514.24869413
CCGTGCG701.0938304E-412.211589
ATATCGT550.003075091212.0880356
GTATTAG3000.011.72798251
GCGGTAG658.029128E-411.69199815
CGGTAGG803.771031E-410.68808516
CGCTAGA803.7744304E-410.68704214
TGTACCG951.6489631E-49.99762255
GTCCTAA2002.5465852E-119.9846331
GTTCTAG2201.8189894E-129.941411
ACACCGT1252.5798836E-69.8776526
GTATTAA5050.09.6033261
GGCGGTA800.00451916749.49959314
TAGCGCT900.0011160949.49820411
GTCTAAT2151.05501385E-109.2880321
GTCCGGG2054.1654857E-109.2772441
TAATACT4100.09.2659394
CTGAGCG1856.730261E-99.2411969
CAGTTCG1054.504593E-49.0456159
CCCCGGT1054.5060564E-49.0453217
Produced by FastQC (version 0.11.5)