FastQCFastQC Report
Wed 25 May 2016
SRR1294552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3077946
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC130140.4228144353409709No Hit
ACCATGTACTCTGCGTTGATACCAC97400.3164447979269292No Hit
GTATCAACGCAGAGTACTTTTTTTT95760.31111656929653736No Hit
GTACATGGTAAGCAGTGGTATCAAC93730.3045212619064792No Hit
GTGGTAAGCAGTGGTATCAACGCAG83670.27183712774688057No Hit
CATGTACTCTGCGTTGATACCACTG69490.22576744361337073No Hit
GGTATCAACGCAGAGTACTTTTTTT65600.21312914521567305No Hit
TATCAACGCAGAGTACTTTTTTTTT60380.19616978335552346No Hit
GGTAAGCAGTGGTATCAACGCAGAG59930.19470776940206228No Hit
GTGGTATCAACGCAGAGTACATGGG53720.1745319768442981No Hit
CCATGTACTCTGCGTTGATACCACT42740.13885883637984553No Hit
ACTCTGCGTTGATACCACTGCTTAC40680.13216606139288994No Hit
GTATCAACGCAGAGTACATGGGAAG40590.1318736586021977No Hit
GCAGTGGTATCAACGCAGAGTACAT37510.12186698532073012No Hit
GAGTACATGGTAAGCAGTGGTATCA35310.1147193615482533No Hit
GCTTACCATGTACTCTGCGTTGATA34350.11160039844753612No Hit
GGTATCAACGCAGAGTACATGGGAA33160.10773418377060545No Hit
GTACATGGGAAGCAGTGGTAAGCAG33160.10773418377060545No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33080.10747427017887903No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA31530.10243844433917944No Hit
GTACTCTGCGTTGATACCACTGCTT31500.10234097674228203No Hit
GTACATGATAAGCAGTGGTATCAAC30800.10006673281467576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCG855.3156677E-511.1787175
ACGGCGT600.0058818811.0823198
TAGACAG3200.010.6896485
CGGACTG1201.5211244E-610.29373555
TAATACT3500.09.7733934
TCTAGAC2002.6375346E-109.5019093
ATAATAC4050.09.3846033
TATACTG5500.09.3291475
GAACCGT1451.7029288E-69.1742576
ACACCGT1252.749228E-59.1218336
TAGGACC2106.657501E-109.04943754
ACACCGC950.00182118219.0018096
ACGGGTC950.00182118219.0018095
CTTCGCG950.00182711518.99859113
TAGAAGT3400.08.9429734
AACACCG850.00742596348.9429735
TTCGCGG850.0074477688.93977714
CTTAGGC1401.0830341E-58.8232013
GTTCTAA1951.6283593E-88.7761281
GTACTGT4450.08.7545696
Produced by FastQC (version 0.11.5)