FastQCFastQC Report
Wed 25 May 2016
SRR1294551_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294551_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772657
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC53440.3014683607714296No Hit
GTACATGGTAAGCAGTGGTATCAAC39800.2245217207841111No Hit
ACCATGTACTCTGCGTTGATACCAC38710.21837275908424472No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA33470.18881261293075874No Hit
GTGGTAAGCAGTGGTATCAACGCAG31210.17606338958975143No Hit
ATTCCATTCCATTCCATTCCATTCC30760.17352482742008182No Hit
GTGGTATCAACGCAGAGTACATGGG28020.15806780443142696No Hit
CATGTACTCTGCGTTGATACCACTG27490.15507794232048275No Hit
GAATGGAATGGAATGGAATGGAATG24800.1399029817951245No Hit
GGTAAGCAGTGGTATCAACGCAGAG23080.1302000330577207No Hit
CCATGTACTCTGCGTTGATACCACT18540.10458876139038742No Hit
GTATCAACGCAGAGTACATGGGAAG18090.10205019922071783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAA601.470391E-615.82992512
GAACGAT400.005283079614.2485416
TCGGAAC400.005286047214.24733413
CGGGTTA551.9649207E-413.8144279
TCTAGAC1104.9847586E-711.2245393
TTAGACC951.3636332E-510.9976314
GTCCTAT1303.209243E-810.9746611
TACATCG700.001494262610.8548065
CTGACCG803.7807188E-410.6845969
GACAGTT1106.0631974E-610.3611137
CTAGCAC1659.331416E-1010.3611133
TAGACTG2300.010.3238635
GTTAGAC856.608698E-410.0563743
CACGTAC856.608698E-410.0563743
CTTACAC2500.09.8775943
CTAAGGC1356.463488E-79.8494533
CTAGACT1752.581146E-99.7693244
CTATACT1752.581146E-99.7693244
TATACTG4500.09.709025
GCCTTAA1606.5118E-89.5113731
Produced by FastQC (version 0.11.5)