FastQCFastQC Report
Wed 25 May 2016
SRR1294551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772657
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC57140.3223409830553796No Hit
GTATCAACGCAGAGTACTTTTTTTT53790.3034427980145059No Hit
GTACATGGTAAGCAGTGGTATCAAC44600.2515997172605868No Hit
ACCATGTACTCTGCGTTGATACCAC39940.22531149568134162No Hit
GGTATCAACGCAGAGTACTTTTTTT39240.2213626211951889No Hit
GTGGTAAGCAGTGGTATCAACGCAG38660.21809069662094813No Hit
TATCAACGCAGAGTACTTTTTTTTT31490.1776429393842125No Hit
ATTCCATTCCATTCCATTCCATTCC30720.17329917744944454No Hit
GTGGTATCAACGCAGAGTACATGGG29410.16590914091107303No Hit
CATGTACTCTGCGTTGATACCACTG29010.16365264120470005No Hit
GGTAAGCAGTGGTATCAACGCAGAG26490.14943669305455032No Hit
GAATGGAATGGAATGGAATGGAATG24920.14057993170703637No Hit
GTATCAACGCAGAGTACATGGGAAG19720.11124543552418771No Hit
CCATGTACTCTGCGTTGATACCACT19060.10752221100867229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGT550.003071640612.08938310
GCACCGT600.005866929411.0856886
GTATTAG2300.010.7459591
TCTGTCG909.528068E-510.5542248
GTCTTGG2800.010.5245461
CTAATAC2350.010.5128713
GGACAGT2100.010.4069716
GTTCTAG1502.4203473E-810.1397771
GTATTGG2250.010.1397771
GTCCAAC750.002633957410.1397771
TCCGTAC951.6462141E-49.9987378
TGGACAG2300.09.9151495
GTATTAC2002.6193447E-109.5060421
CCTAGAC1606.611481E-89.5020173
TCTACAC1804.1654857E-99.5020173
TCGCCCA1002.7588222E-49.49880112
TATACTG3950.09.3817395
GTCTTAC1554.2200372E-79.1993941
GTGTAGA2501.8189894E-129.1257991
GGCTTAC1151.1028584E-49.0927341
Produced by FastQC (version 0.11.5)