FastQCFastQC Report
Wed 25 May 2016
SRR1294550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105469
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC84830.4029031061487963No Hit
ACCATGTACTCTGCGTTGATACCAC81200.3856622918694125No Hit
GTACATGGTAAGCAGTGGTATCAAC80760.3835724961991841No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA60880.28915172818977625No Hit
GTGGTAAGCAGTGGTATCAACGCAG59300.2816474619194108No Hit
GTGGTATCAACGCAGAGTACATGGG47980.22788271876717256No Hit
CATGTACTCTGCGTTGATACCACTG46250.2196660221546838No Hit
GGTAAGCAGTGGTATCAACGCAGAG42080.1998604586436561No Hit
GTATCAACGCAGAGTACTTTTTTTT37070.17606528521673792No Hit
CCATGTACTCTGCGTTGATACCACT35110.1667561954129935No Hit
GTATCAACGCAGAGTACATGGGAAG31050.14747308081952287No Hit
GCTTACCATGTACTCTGCGTTGATA30580.14524079908086987No Hit
GAGTACATGGTAAGCAGTGGTATCA29000.13773653281050446No Hit
GGTATCAACGCAGAGTACATGGGAA28650.1360741953455501No Hit
ACTCTGCGTTGATACCACTGCTTAC28060.13327196933319846No Hit
GGTATCAACGCAGAGTACTTTTTTT25490.12106566280481926No Hit
GTACATGGGAAGCAGTGGTAAGCAG25250.11992577425742199No Hit
GCAGTGGTATCAACGCAGAGTACAT24660.11712354824507033No Hit
GCGTTGATACCACTGCTTACCATGT23470.1114716008642255No Hit
TATCAACGCAGAGTACTTTTTTTTT23240.11037920767296978No Hit
GCTTACCACTGCTTCCCATGTACTC22880.10866937485187386No Hit
GTACTCTGCGTTGATACCACTGCTT22420.10648458846936242No Hit
CTGCTTACCATGTACTCTGCGTTGA21390.10159256678678241No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA21290.10111761322536689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1052.0028892E-812.6630564
TACACCG550.00307562612.0874635
GTCTAAG1354.651156E-911.271581
ACACCGT600.005887231311.0807016
GTATAGG1900.011.0120361
CGCCTTG803.7726972E-410.68724614
TATACTG3950.010.0983875
GTCTTAG2002.5465852E-119.9859151
GTCTAAC1151.0134345E-59.923891
CGCCTCC1554.0778104E-89.80435511
GTTCTAA2450.09.7044841
GTACGGA800.00447600669.5103951
GACTGTC1106.857351E-59.4975187
ACTGGCC2201.6370905E-119.49729258
CTTGGAC1401.0632339E-69.49706653
CCTGGAC2357.2759576E-129.2950013
TAGACTG2054.2564352E-109.2656515
CTAGACA2600.09.1320114
GTATTAG2950.09.0268151
CCGCCTT850.0074481878.93947813
Produced by FastQC (version 0.11.5)