FastQCFastQC Report
Wed 25 May 2016
SRR1294550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105469
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT136590.648739069537476No Hit
GGTATCAACGCAGAGTACTTTTTTT94370.4482136759078381No Hit
CCCATGTACTCTGCGTTGATACCAC90270.4287405798898013No Hit
ACCATGTACTCTGCGTTGATACCAC86000.4084600628173581No Hit
GTACATGGTAAGCAGTGGTATCAAC85220.4047554250383169No Hit
TATCAACGCAGAGTACTTTTTTTTT81410.3866596943483851No Hit
GTGGTAAGCAGTGGTATCAACGCAG65230.3098122081113519No Hit
CATGTACTCTGCGTTGATACCACTG51460.2444111027044331No Hit
GTGGTATCAACGCAGAGTACATGGG48870.23210980546377077No Hit
GGTAAGCAGTGGTATCAACGCAGAG47670.2264103627267844No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43530.20674728528418135No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39130.1858493285818979No Hit
CCATGTACTCTGCGTTGATACCACT36340.17259812421840456No Hit
GCTTACCATGTACTCTGCGTTGATA32180.1528400560635184No Hit
GTATCAACGCAGAGTACATGGGAAG32070.1523176071459613No Hit
GAGTACATGGTAAGCAGTGGTATCA31520.14970536255817588No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30800.14628569691598406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA30550.14509831301244522No Hit
GGTATCAACGCAGAGTACATGGGAA30140.14315100341064152No Hit
ACTCTGCGTTGATACCACTGCTTAC30040.142676049849226No Hit
GCAGTGGTATCAACGCAGAGTACAT28390.13483931608586971No Hit
GTACATGGGAAGCAGTGGTAAGCAG27470.13046974332084682No Hit
GCGTTGATACCACTGCTTACCATGT23360.1109491519466684No Hit
GTACTCTGCGTTGATACCACTGCTT23180.11009423553612047No Hit
GCTTACCACTGCTTCCCATGTACTC23020.10933430983785561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG604.069331E-412.6754141
GTGCGCC550.003071769612.08948811
GTGCTAG2400.011.4870941
CTTAGAC1701.382432E-1010.61967853
GTCTTAA2100.010.4119471
TAGTACT2250.010.1352724
TCTATAC2350.010.1083163
GTCTAAG1554.02124E-89.8132241
GTCAAAG1401.0488548E-69.5065591
TACCCTG2106.730261E-119.5018185
TTAGTAC900.00111191179.5018173
TGCTAGG2357.2759576E-129.2996512
GTTCTAA2252.7284841E-119.2953031
TTAGGGT1651.0626354E-79.2138834
GTAGTAC1451.702072E-69.1741693
CTGTCGC2600.09.1341939
CTGATCA2304.1836756E-119.0865369
CTAGACC1054.48455E-49.049354
GGACTTA1054.48455E-49.049356
GTCCTAC950.00181259539.0062151
Produced by FastQC (version 0.11.5)