FastQCFastQC Report
Wed 25 May 2016
SRR1294549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737805
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC50040.28794945347723133No Hit
ACCATGTACTCTGCGTTGATACCAC42270.24323787766751737No Hit
GTACATGGTAAGCAGTGGTATCAAC41520.23892208849669552No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA35540.20451086284134298No Hit
GTGGTAAGCAGTGGTATCAACGCAG30430.1751059526241437No Hit
GTGGTATCAACGCAGAGTACATGGG26780.15410244532614417No Hit
ATTCCATTCCATTCCATTCCATTCC26360.15168560339048398No Hit
CATGTACTCTGCGTTGATACCACTG26050.1499017438665443No Hit
GGTAAGCAGTGGTATCAACGCAGAG23360.13442244670719672No Hit
GAATGGAATGGAATGGAATGGAATG20910.12032420208251213No Hit
CCATGTACTCTGCGTTGATACCACT18510.10651367673588233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCG755.8356818E-815.2023262
TTAGACA1603.6379788E-1211.8740834
GGCTTAC700.001477886310.869131
ATTAGAC1606.180926E-910.0926793
GTTCTAT1356.3451625E-79.862731
GTCCTAG2054.0017767E-119.7424541
TTTATAC3050.09.6547143
TAATACT3450.09.6369364
CCCCTAG1304.178688E-69.5104891
AGCCTCG1201.697125E-59.5014542
CTAGACT1356.852384E-69.1474414
GCCGCGG1054.4968908E-49.046663
ACGTACT850.00744021268.9404864
ATTATAC3000.08.86572553
TGCACTG3600.08.7069085
CTGTCGC1201.7688374E-48.7059079
ACTGGTC1201.7688374E-48.7059078
GTGTAGA2303.947207E-108.6834911
TCTAGAC1651.0510776E-68.63544753
TCAGAAC2201.6061676E-98.63544753
Produced by FastQC (version 0.11.5)