FastQCFastQC Report
Wed 25 May 2016
SRR1294549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737805
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55880.3215550651540305No Hit
CCCATGTACTCTGCGTTGATACCAC52580.3025655928024145No Hit
GTACATGGTAAGCAGTGGTATCAAC44670.25704840301414716No Hit
ACCATGTACTCTGCGTTGATACCAC44380.25537963120142937No Hit
GGTATCAACGCAGAGTACTTTTTTT41590.23932489548597222No Hit
GTGGTAAGCAGTGGTATCAACGCAG33320.1917361268957104No Hit
TATCAACGCAGAGTACTTTTTTTTT33220.19116068833960081No Hit
GTGGTATCAACGCAGAGTACATGGG28850.16601402343761237No Hit
ATTCCATTCCATTCCATTCCATTCC27510.1583031467857441No Hit
CATGTACTCTGCGTTGATACCACTG27450.15795788365207833No Hit
GGTAAGCAGTGGTATCAACGCAGAG26610.15312419978075792No Hit
GAATGGAATGGAATGGAATGGAATG21890.12596349993238598No Hit
CCATGTACTCTGCGTTGATACCACT20320.11692911460146563No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17940.10323367696605774No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17800.10242806298750434No Hit
GAGTACATGGTAAGCAGTGGTATCA17540.10093192274161947No Hit
GTATCAACGCAGAGTACATGGGAAG17490.10064420346356467No Hit
GCTTACCATGTACTCTGCGTTGATA17400.10012630876306607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGGA500.001501350113.29802711
CTAGGAC1403.6379788E-1212.8953963
TGCACCG550.003064173512.0932915
CTCGGAA658.034258E-411.69057212
TTATACT2000.011.40224554
GGAACGA1002.4027528E-510.44844915
GACTTCC1106.039483E-610.3644857
TTAGAAC1201.5197693E-610.2936943
ATAGAAC1201.5197693E-610.2936943
GTCTAAT1856.002665E-1110.2761381
TAGGACA2053.6379788E-1210.197134
TCCGTAC1054.0960927E-59.9543413
CTTACAC2400.09.8977823
CCCTTAC1651.0122676E-89.793471
CTCCTAT1951.6189006E-109.7491561
GTATTAG2550.09.6918081
GTCTAGA1304.207117E-69.5054271
GTTACAC1502.639863E-79.5018713
TGGACTG2550.09.3155595
CTAGACA2054.2200554E-109.2701194
Produced by FastQC (version 0.11.5)