FastQCFastQC Report
Wed 25 May 2016
SRR1294548_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294548_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073783
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC437412.1092370802538163No Hit
ACCATGTACTCTGCGTTGATACCAC321411.5498728651937064No Hit
GTACATGGTAAGCAGTGGTATCAAC305121.471320769820179No Hit
GTGGTAAGCAGTGGTATCAACGCAG238581.1504578830089744No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA229181.1051300931678965No Hit
CATGTACTCTGCGTTGATACCACTG226051.090036903571878No Hit
GGTAAGCAGTGGTATCAACGCAGAG189450.9135478495098088No Hit
ACTCTGCGTTGATACCACTGCTTAC152000.7329600059408337No Hit
GTATCAACGCAGAGTACATGGGAAG150950.7278967953734793No Hit
CCATGTACTCTGCGTTGATACCACT140750.6787113212906075No Hit
GTACTCTGCGTTGATACCACTGCTT132880.6407613525619604No Hit
GGTATCAACGCAGAGTACATGGGAA124800.6017987417198424No Hit
GCAGTGGTATCAACGCAGAGTACAT114110.550250436038872No Hit
GTACATGGGAAGCAGTGGTAAGCAG109190.5265256779518397No Hit
GCTTACCATGTACTCTGCGTTGATA107800.5198229515817229No Hit
GAGTACATGGTAAGCAGTGGTATCA106820.5150972883855254No Hit
GTGGTATCAACGCAGAGTACATGGG102650.49498910927517487No Hit
TATCAACGCAGAGTACATGGGAAGC97770.4714572354002323No Hit
GTACATGATAAGCAGTGGTATCAAC97630.47078214065791846No Hit
GCGTTGATACCACTGCTTACCATGT97260.4689979616960887No Hit
GCGTTGATACCACTGCTTACCACTG93480.45077040365361276No Hit
ATCATGTACTCTGCGTTGATACCAC93060.44874511942667095No Hit
GCTTACCACTGCTTCCCATGTACTC91450.44098152989006084No Hit
CTGCTTACCATGTACTCTGCGTTGA88280.42569545608195264No Hit
GTACATGGGAAGCAGTGGTATCAAC87180.42039114024948604No Hit
AAGCAGTGGTATCAACGCAGAGTAC86660.41788364549232004No Hit
GTATCAACGCAGAGTACATGGTAAG80080.3861541926035656No Hit
ATGGTAAGCAGTGGTATCAACGCAG70220.3386082343234562No Hit
TATCAACGCAGAGTACATGGTAAGC69620.3357149711421108No Hit
CAGTGGTATCAACGCAGAGTACATG68430.32997666583244245No Hit
GAGTAAGCAGTGGTATCAACGCAGA67720.3265529710678504No Hit
ACGCAGAGTACATGGTAAGCAGTGG66380.32009134996284566No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA62320.3005136024357418No Hit
GGTATCAACGCAGAGTACATGGTAA55550.2678679495395613No Hit
ACGCAGAGTACATGGGAAGCAGTGG53770.25928460210156995No Hit
GTGATAAGCAGTGGTATCAACGCAG52390.2526300967844755No Hit
TACCACTGCTTACCATGTACTCTGC50020.2412017072181612No Hit
GTGGTATCAACGCAGAGTACATGGT47900.23097884397740748No Hit
GTACATAAGCAGTGGTATCAACGCA45440.21911646493389136No Hit
GAGTACATGGGAAGCAGTGGTAAGC44170.2129923912000436No Hit
AAAAAGTACTCTGCGTTGATACCAC43990.21212441224563997No Hit
GTAAGCAGTGGTATCAACGCAGAGT40930.19736877002077846No Hit
GTATCAACGCAGAGTACTTTTTTTT40220.1939450752561864No Hit
GTACATGGTAAGCAGTGGTAAGCAG39820.19201623313528948No Hit
GCTTACTCTGCGTTGATACCACTGC39550.19071426470368405No Hit
GAGTACATGATAAGCAGTGGTATCA37000.1784178961829661No Hit
GAGTACATGGGAAGCAGTGGTATCA36880.17783924354669703No Hit
AAAGTACTCTGCGTTGATACCACTG36730.17711592775136067No Hit
GATAAGCAGTGGTATCAACGCAGAG36620.17658549616811403No Hit
GCAGAGTACATGGTAAGCAGTGGTA36440.1757175172137104No Hit
GCTTCCCATGTACTCTGCGTTGATA36000.17359579088072377No Hit
ACTCTGCGTTGATACCACTGCTTAT34420.16597686450318092No Hit
CTTCCCATGTACTCTGCGTTGATAC33730.1626496118446337No Hit
GCTTACCACTGCTTACCATGTACTC33230.16023855919351251No Hit
GCTTATCATGTACTCTGCGTTGATA32880.15855082233772771No Hit
TGGTAAGCAGTGGTATCAACGCAGA31520.15199275912667815No Hit
GCGTTGATACCACTGCTTATCATGT30430.146736664347234No Hit
CTGCTTACCACTGCTTCCCATGTAC29610.1427825379993953No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT29160.14061259061338627No Hit
GTTGATACCACTGCTTACCATGTAC29010.1398892748180499No Hit
GCGTTGATACCACTGCTTACTCTGC28760.13868374849248932No Hit
TATCAACGCAGAGTACTTTTTTTTT28720.13849086428039964No Hit
GCGTTGATACCACTGCTTCCCATGT28520.1375264432199512No Hit
GCTTATGTACTCTGCGTTGATACCA26700.12875021156987013No Hit
GATACCACTGCTTACCATGTACTCT26680.12865376946382528No Hit
GGTATCAACGCAGAGTACTTTTTTT25530.12310834836624662No Hit
ATACCACTGCTTACCATGTACTCTG24660.1189131167532958No Hit
AAACAAAAAAAAAAAAAAAAAAAAA24410.11770759042773521No Hit
CTGCTTCCCATGTACTCTGCGTTGA23550.11356057986780682No Hit
CCACTGCTTACCATGTACTCTGCGT23240.11206572722411168No Hit
CTGCTTACTCTGCGTTGATACCACT23220.11196928511806684No Hit
GTTGATACCACTGCTTACCACTGCT22760.10975111667903537No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT22250.10729184297489178No Hit
GTATCAACGCAGAGTACATGGGAAA22050.10632742191444332No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21730.10478434821772577No Hit
GTACATGGGAAGTAAGCAGTGGTAT21210.10227685346055974No Hit
GCAGTAAGCAGTGGTATCAACGCAG21160.10203574819544765No Hit
TGATACCACTGCTTACCATGTACTC21150.10198752714242522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA8450.014.0662011
TAAAGGT551.9641993E-413.8152354
CGAAACA500.001494083713.30679117
GTGTCGA500.001501435613.29812613
TTAGACT500.001502254413.2971644
CTATTAG655.4072803E-513.1659641
ATAGGAC655.4688353E-513.1500913
TAGGACC604.1004215E-412.6639654
GCGTACA701.0919378E-412.21344952
GACTAAA550.00304794712.1020481
CGCGGTG550.003072281112.08920512
CATGCGG550.003073946312.0883310
GCGAAAA2700.011.9739841
GCGATAA802.8405473E-511.8859411
GTTCATA802.8405473E-511.8859411
CAATTAG657.9636456E-411.7030791
TAGCGAA1302.5956979E-911.69827818
CTTAGTA1302.6193447E-911.6915072
ACCCCTT658.0381474E-411.6900976
TTAGCGA1157.027484E-811.57112217
Produced by FastQC (version 0.11.5)