FastQCFastQC Report
Wed 25 May 2016
SRR1294548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073783
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC462242.2289699549084934No Hit
ACCATGTACTCTGCGTTGATACCAC335051.6156463815162916No Hit
GTACATGGTAAGCAGTGGTATCAAC312951.5090778543367362No Hit
GTGGTAAGCAGTGGTATCAACGCAG278041.3407381582354567No Hit
CATGTACTCTGCGTTGATACCACTG235941.1377275250110546No Hit
GGTAAGCAGTGGTATCAACGCAGAG217081.0467826190107645No Hit
ACTCTGCGTTGATACCACTGCTTAC160360.7732728062675795No Hit
GTATCAACGCAGAGTACATGGGAAG159410.7686918062304494No Hit
CCATGTACTCTGCGTTGATACCACT150050.7235569006014613No Hit
GTATCAACGCAGAGTACTTTTTTTT140750.6787113212906075No Hit
GTACTCTGCGTTGATACCACTGCTT131780.6354570367294938No Hit
GGTATCAACGCAGAGTACATGGGAA131390.6335764156616194No Hit
GCAGTGGTATCAACGCAGAGTACAT125100.6032453733105151No Hit
GTACATGGGAAGCAGTGGTAAGCAG117330.5657776151120922No Hit
GAGTACATGGTAAGCAGTGGTATCA113490.5472607307514817No Hit
GTGGTATCAACGCAGAGTACATGGG110310.531926435890351No Hit
GCTTACCATGTACTCTGCGTTGATA107970.5206427094831041No Hit
TATCAACGCAGAGTACATGGGAAGC104470.5037653409252559No Hit
AAGCAGTGGTATCAACGCAGAGTAC103060.49696617244909425No Hit
GTACTTTTTTTTTTTTTTTTTTTTT102630.49489266716913No Hit
GCGTTGATACCACTGCTTACCATGT100840.4862610986781163No Hit
GTACATGATAAGCAGTGGTATCAAC99960.48201764601214303No Hit
GCGTTGATACCACTGCTTACCACTG97330.4693355090672457No Hit
ATCATGTACTCTGCGTTGATACCAC96040.4631149932273531No Hit
TATCAACGCAGAGTACTTTTTTTTT95280.4594501931976489No Hit
GCTTACCACTGCTTCCCATGTACTC91930.4432961404351371No Hit
GTACATGGGAAGCAGTGGTATCAAC90750.43760605617849124No Hit
CTGCTTACCATGTACTCTGCGTTGA90620.4369791824891997No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA87470.4217895507871363No Hit
GGTATCAACGCAGAGTACTTTTTTT86770.4184140770755667No Hit
GTATCAACGCAGAGTACATGGTAAG82030.39555729794293804No Hit
CAGTGGTATCAACGCAGAGTACATG77240.37245941354519735No Hit
ACGCAGAGTACATGGTAAGCAGTGG74830.3608381397667933No Hit
ATGGTAAGCAGTGGTATCAACGCAG74460.3590539608049637No Hit
TATCAACGCAGAGTACATGGTAAGC72390.34907220282932205No Hit
GAGTAAGCAGTGGTATCAACGCAGA72380.3490239817762996No Hit
ACGCAGAGTACATGGGAAGCAGTGG60380.29115871814939176No Hit
GAGTACTTTTTTTTTTTTTTTTTTT59530.28705992864248575No Hit
GGTATCAACGCAGAGTACATGGTAA57910.27924811805285316No Hit
GTGATAAGCAGTGGTATCAACGCAG56670.2732687074780727No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52900.25508937048861907No Hit
TACCACTGCTTACCATGTACTCTGC50830.2451076125129775No Hit
GTGGTATCAACGCAGAGTACATGGT49060.2365724861280086No Hit
GAGTACATGGGAAGCAGTGGTAAGC47670.22986975975789176No Hit
GTAAGCAGTGGTATCAACGCAGAGT45780.22075598073665373No Hit
GTACATAAGCAGTGGTATCAACGCA45630.22003266494131737No Hit
GTACATGGTAAGCAGTGGTAAGCAG44620.21516233858605263No Hit
AAAAAGTACTCTGCGTTGATACCAC44440.214294359631649No Hit
GCTTACTCTGCGTTGATACCACTGC42410.2045054858680971No Hit
GTGGTATCAACGCAGAGTACTTTTT41360.19944227530074266No Hit
GATAAGCAGTGGTATCAACGCAGAG40680.1961632436952179No Hit
GAGTACATGGGAAGCAGTGGTATCA39180.18893008574185438No Hit
GCAGAGTACATGGTAAGCAGTGGTA38750.1868565804618902No Hit
AAAGTACTCTGCGTTGATACCACTG37580.18121471725826666No Hit
GAGTACATGATAAGCAGTGGTATCA37170.1792376540843473No Hit
GCTTCCCATGTACTCTGCGTTGATA37150.17914121197830243No Hit
CTTCCCATGTACTCTGCGTTGATAC36940.17812856986483158No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT36700.17697126459229343No Hit
ACTCTGCGTTGATACCACTGCTTAT36520.1761032856378898No Hit
GCTTACCACTGCTTACCATGTACTC34950.16853258031336935No Hit
TGGTAAGCAGTGGTATCAACGCAGA33910.16351759079903733No Hit
GCTTATCATGTACTCTGCGTTGATA32120.15488602230802354No Hit
CTGCTTACCACTGCTTCCCATGTAC31430.15155876964947634No Hit
GCGTTGATACCACTGCTTATCATGT31090.14991925384671395No Hit
GTTGATACCACTGCTTACCATGTAC29210.1408536958784984No Hit
GATACCACTGCTTACCATGTACTCT29140.14051614850734143No Hit
GCGTTGATACCACTGCTTACTCTGC29090.1402750432422293No Hit
GCGTTGATACCACTGCTTCCCATGT29010.1398892748180499No Hit
GCAGTGGTATCAACGCAGAGTACTT28890.13931062218178084No Hit
GCTTATGTACTCTGCGTTGATACCA27770.13390986424326942No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA26970.13005218000147556No Hit
ATACCACTGCTTACCATGTACTCTG26430.1274482431382647No Hit
GTATCAACGCAGAGTACATGGGAAA26210.12638737997177138No Hit
GCAGTAAGCAGTGGTATCAACGCAG25710.12397632732065024No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT25450.12272257994206723No Hit
GCAGTGGTAAGCAGTGGTATCAACG25100.12103484308628241No Hit
CCACTGCTTACCATGTACTCTGCGT24990.12050441150303576No Hit
CTGCTTCCCATGTACTCTGCGTTGA24460.11794869569284733No Hit
CTGCTTACTCTGCGTTGATACCACT23890.11520009567056919No Hit
GTTGATACCACTGCTTACCACTGCT23430.11298192723153773No Hit
GCAGAGTACTTTTTTTTTTTTTTTT23050.11114952721668564No Hit
CATGGTAAGCAGTGGTATCAACGCA22950.1106673166864614No Hit
ATCAACGCAGAGTACATGGGAAGCA22310.10758116929302632No Hit
GTACATGGGAAGTAAGCAGTGGTAT22200.10705073770977966No Hit
AAGCAGTGGTAAGCAGTGGTATCAA21530.10381992715727731No Hit
TGATACCACTGCTTACCATGTACTC21500.10367526399821002No Hit
ATGATAAGCAGTGGTATCAACGCAG21470.10353060083914277No Hit
GCTAAGCAGTGGTATCAACGCAGAG20800.1002997902866404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAA504.5417673E-617.1086851
TAGACAG702.4989276E-816.2884855
CGCTAAG601.4647903E-615.8360272
CTTAAGG551.1268536E-515.5480993
ATTAGAC400.005273937714.2524233
CCTAGTA400.005273937714.2524232
AGCGGCC400.005281537314.24933058
CGCCCTA655.4627806E-513.15164316
GAACAAA4000.012.8315141
CCCCGTA905.4157135E-712.66515615
CCGTACA905.4188786E-712.66454517
GTTTAGG701.08607914E-412.22048951
GTCATAT550.003057390212.0970511
TAATACT1103.8071448E-812.0929664
CCTACAC550.003065133512.0929663
GCCCCGT951.0415151E-611.998857514
CCCTGAC752.0711777E-411.4019392
CTAGGGT855.3154006E-511.1783724
TTTTTAC4000.011.168171
TATCGCG600.00587778111.0830797
Produced by FastQC (version 0.11.5)