FastQCFastQC Report
Wed 25 May 2016
SRR1294547_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294547_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571676
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGTACTCTGCGTTGATACCAC90080.3502774066406499No Hit
GTACATGGTAAGCAGTGGTATCAAC86160.33503442890939605No Hit
CCCATGTACTCTGCGTTGATACCAC81970.3187415522017548No Hit
GTGGTAAGCAGTGGTATCAACGCAG51880.201736144055472No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA48200.1874264098587847No Hit
CATGTACTCTGCGTTGATACCACTG47660.18532661190600994No Hit
GGTAAGCAGTGGTATCAACGCAGAG43600.1695392421129256No Hit
GTGGTATCAACGCAGAGTACATGGG39580.15390741290893564No Hit
CCATGTACTCTGCGTTGATACCACT37930.14749136360879053No Hit
GAGTACATGGTAAGCAGTGGTATCA31900.12404361980280564No Hit
ACTCTGCGTTGATACCACTGCTTAC30880.12007733478089774No Hit
GCTTACCATGTACTCTGCGTTGATA30870.12003844963362414No Hit
GTATCAACGCAGAGTACATGGGAAG27670.10759520250606996No Hit
ATTCCATTCCATTCCATTCCATTCC27030.10510655308055915No Hit
GCGTTGATACCACTGCTTACCATGT26290.10222905218231224No Hit
GCAGTGGTATCAACGCAGAGTACAT26110.10152911953138731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA1701.364242E-1010.6312631
TATACTG4600.010.3234445
TTAGACT2054.1836756E-119.7294064
TAGACTG2850.09.6641935
GTCTAGC900.00110105229.5121831
TCGTGGG1502.658944E-79.49775412
GTATAAT3700.09.2550961
GATTAGG1054.436789E-49.0592211
TCTAGAC2106.712071E-109.045483
GCGTGCA850.00745433948.9387149
TAATACT4150.08.9255994
GGAACGA1304.3784486E-58.76783915
GCCTTAT1856.4426786E-88.7409251
GAGTGTC2502.5465852E-118.7375947
CTTAGAC1201.7688987E-48.7062753
CTAGACT1752.635934E-78.68366054
TTGGACT2304.0563464E-108.6718634
TAAGACT2304.0563464E-108.6718634
GTCCTAC2201.5679689E-98.6474391
GTATTAT5650.08.5862181
Produced by FastQC (version 0.11.5)