FastQCFastQC Report
Wed 25 May 2016
SRR1294547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571676
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGTACTCTGCGTTGATACCAC95120.36987552086654774No Hit
GTACATGGTAAGCAGTGGTATCAAC88570.3444057494023353No Hit
CCCATGTACTCTGCGTTGATACCAC84740.3295127379965439No Hit
GTATCAACGCAGAGTACTTTTTTTT78620.3057150278650965No Hit
GTGGTAAGCAGTGGTATCAACGCAG56700.22047878504135046No Hit
GGTATCAACGCAGAGTACTTTTTTT51930.20193056979184004No Hit
CATGTACTCTGCGTTGATACCACTG50370.19586448681715735No Hit
GGTAAGCAGTGGTATCAACGCAGAG47640.1852488416114627No Hit
TATCAACGCAGAGTACTTTTTTTTT46440.18058262393862992No Hit
CCATGTACTCTGCGTTGATACCACT40470.15736819101628666No Hit
GTGGTATCAACGCAGAGTACATGGG40000.15554058909442714No Hit
ACTCTGCGTTGATACCACTGCTTAC32910.12797101967743993No Hit
GCTTACCATGTACTCTGCGTTGATA32350.12579345143011794No Hit
GAGTACATGGTAAGCAGTGGTATCA32080.12474355245373056No Hit
ATTCCATTCCATTCCATTCCATTCC28590.11117263605524179No Hit
GTATCAACGCAGAGTACATGGGAAG28140.1094228044279295No Hit
GCAGTGGTATCAACGCAGAGTACAT27990.1088395272188254No Hit
GCGTTGATACCACTGCTTACCATGT27140.10553428970056881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC400.00528034814.257
AAGACCG604.091631E-412.6674065
AAGCCGA658.0213184E-411.6932176
CCCGTAA600.005850262511.0902341
ACAGTCG600.00587743111.0833348
TCAGGAC2550.010.8045523
GTCCTAG1909.094947E-1210.5065381
CTAGGAC2053.6379788E-1210.1957163
GTTCTAG2400.09.9019951
GTCTAGG1554.0274244E-89.8125581
GTATTAG3000.09.5059161
CTTGGAC2401.8189894E-129.5005543
GCTAGAC1106.835002E-59.5005543
ACAGCGC1002.7564118E-49.5000018
TATTCCG1252.7533011E-59.1205325
CTGTCCG1252.755195E-59.129
TTTATAC4200.09.0481473
TCTACAC3150.09.0481463
GTCTTAA2850.09.0056041
TCTAGGA2850.09.0001752
Produced by FastQC (version 0.11.5)