FastQCFastQC Report
Wed 25 May 2016
SRR1294546_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294546_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523155
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG31840.20903978912192128No Hit
GTATCAACGCAGAGTACTTTTTTTT23510.15435067343770004No Hit
CCCATGTACTCTGCGTTGATACCAC21980.14430573382223083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA20030.13150335980251518No Hit
GTACATGGTAAGCAGTGGTATCAAC18920.12421585459129242No Hit
ACCATGTACTCTGCGTTGATACCAC18170.11929186458370948No Hit
GGTATCAACGCAGAGTACTTTTTTT16940.11121652097127344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC350.002173683416.2817758
GTCCTGG2700.011.2731911
TGGCGCG600.00588040511.08210714
ACTCGAT600.00588471911.0810167
GTACTAG1002.3657702E-510.4629321
GTCTTAG856.5182394E-410.0712711
GTATAGG1803.8562575E-1010.0401871
TAGACTG2107.2759576E-129.9496465
GCGCGAT800.0045170339.49957416
GGTGTCC900.00111595299.4977028
ACCGTGT800.0045243819.4977028
CTAGACT1701.6705599E-89.497394
ACTAGAC900.00111628819.497393
GGACCAT800.00452560659.497396
GTTTAGA1752.6137059E-89.2399921
ATAGGAC1356.867529E-69.1456353
GTGTTAA2401.0913936E-119.1154331
ATTACCC1054.503675E-49.0451333
CCGGGTT950.00181491259.00462317
CTTGCTC3000.08.864233
Produced by FastQC (version 0.11.5)