FastQCFastQC Report
Wed 25 May 2016
SRR1294546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294546_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523155
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87260.5728898240822503No Hit
GGTATCAACGCAGAGTACTTTTTTT67210.4412551578795329No Hit
TATCAACGCAGAGTACTTTTTTTTT51110.3355535057167524No Hit
GTGGTATCAACGCAGAGTACATGGG32600.2140294323296053No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27830.18271285588137778No Hit
CCCATGTACTCTGCGTTGATACCAC21510.14122003341747885No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20870.13701822861100807No Hit
GTACATGGTAAGCAGTGGTATCAAC20200.13261946420423398No Hit
ACCATGTACTCTGCGTTGATACCAC19450.12769547419665103No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16820.11042868257006018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC658.0014096E-411.6961093
GTCTTGC3850.010.8653131
GCTAGAC803.759271E-410.6909743
GTCTAGG1303.8002145E-710.2384671
CAGGACT1803.9108272E-1010.0310384
GTCCTAC951.6313423E-410.00752451
CTTGCTC3800.010.0032513
CCTAGAC1054.0894287E-59.9556163
GTCTATA1554.0134182E-89.813831
TAGACTG2350.09.7052815
GTCCTAA1606.553273E-89.5071491
GCCCTAT900.00110585089.5071491
CTAATAC2303.6379788E-129.5030883
TCAGTCC900.00111385529.4996568
TCTGTCG1651.06529114E-79.2117878
CTGTCGC1554.259382E-79.1932149
TTACACC1356.815473E-69.1511224
CCCGGGT1356.875358E-69.14481316
CTAGACC1151.1063235E-49.08991054
GTATTAG2201.6552804E-109.0750061
Produced by FastQC (version 0.11.5)