FastQCFastQC Report
Wed 25 May 2016
SRR1294545_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294545_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940632
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC59120.3046430235098669No Hit
ACCATGTACTCTGCGTTGATACCAC53280.2745497343133577No Hit
GTACATGGTAAGCAGTGGTATCAAC52060.26826312252915546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA44730.23049192221915332No Hit
GTGGTAAGCAGTGGTATCAACGCAG35510.1829816266041166No Hit
GTGGTATCAACGCAGAGTACATGGG32420.16705897872445677No Hit
CATGTACTCTGCGTTGATACCACTG31590.16278202152700771No Hit
GGTAAGCAGTGGTATCAACGCAGAG27560.1420155907972248No Hit
CCATGTACTCTGCGTTGATACCACT24240.12490776200742851No Hit
ATTCCATTCCATTCCATTCCATTCC22590.11640537721731889No Hit
ACTCTGCGTTGATACCACTGCTTAC20150.10383215364891438No Hit
GAGTACATGGTAAGCAGTGGTATCA19690.10146179182864139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG1406.675691E-1011.5490321
GTCTTAA1701.364242E-1010.6299051
GGCTAGA1355.5930286E-810.5677421
CTAGTAC909.537519E-510.5533083
CGTACTC951.6469807E-49.9983865
ACCGCTT951.6478375E-49.997878
GTCCTAC1252.532599E-69.8914061
ACGTACT1106.849119E-59.49846654
CTTGTAC1606.6567736E-89.4979763
ATCCGAA800.0045242459.49797612
TAGGACA2054.2382453E-109.2667964
GTCCTAG1856.5647328E-99.2539141
TCTAGAC1651.0690201E-79.2101593
TAGCACC1451.7099501E-69.1709334
CGCTTCC1252.7610182E-59.11805810
GTCTAAC1151.0967926E-49.0974471
GTACTAG1151.0967926E-49.0974471
GTGTAAG3400.08.9514991
ACCGCGC850.00740673958.945497518
TATTCCG850.00744600048.93973355
Produced by FastQC (version 0.11.5)