FastQCFastQC Report
Wed 25 May 2016
SRR1294545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940632
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66760.3440116415683138No Hit
CCCATGTACTCTGCGTTGATACCAC60790.3132484675095536No Hit
GTACATGGTAAGCAGTGGTATCAAC56020.28866884602541854No Hit
ACCATGTACTCTGCGTTGATACCAC55480.2858862473668372No Hit
GGTATCAACGCAGAGTACTTTTTTT48690.25089764571541645No Hit
GTGGTAAGCAGTGGTATCAACGCAG41200.21230197172879764No Hit
TATCAACGCAGAGTACTTTTTTTTT39570.2039026461482651No Hit
CATGTACTCTGCGTTGATACCACTG32580.16788345240107347No Hit
GTGGTATCAACGCAGAGTACATGGG31990.16484320571854943No Hit
GGTAAGCAGTGGTATCAACGCAGAG31400.16180295903602537No Hit
CCATGTACTCTGCGTTGATACCACT24710.12732965343249003No Hit
ATTCCATTCCATTCCATTCCATTCC22210.11444725223535425No Hit
GAGTACATGGTAAGCAGTGGTATCA21830.11248912725338961No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20820.10728463716974676No Hit
ACTCTGCGTTGATACCACTGCTTAC20760.1069754595410155No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20430.10527498258299359No Hit
GCTTACCATGTACTCTGCGTTGATA20080.10347144641539457No Hit
GTATCAACGCAGAGTACATGGGAAG19980.10295615036750914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCC550.00307097712.0898279
GTCCTAA1451.202352E-911.1467011
CGTGTGC700.001492806210.8561718
TTAGACA1855.456968E-1210.7869884
TAACCCG803.761245E-410.6906765
TGCACCG803.761245E-410.6906765
CTATACT2700.010.5586924
TCTATAC1803.45608E-1110.5586923
ATACTGT3750.010.3900216
CTAGAAC2105.456968E-129.95533853
TCAGGAC1951.6189006E-109.7464853
GTCCTAG2054.0017767E-119.7393691
TATACTG4150.09.6173145
CTGATCA3000.09.4991499
CTATTAG1752.6353518E-89.2358371
AATACTG3200.09.205865
GCCTTAT1356.779159E-69.1553521
GGACAGT2600.09.1375666
CTTAGAC1252.7444692E-59.1227093
GTTTAGG1054.4575223E-49.0547431
Produced by FastQC (version 0.11.5)