FastQCFastQC Report
Wed 25 May 2016
SRR1294544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509776
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC37170.24619546210828627No Hit
GTGGTATCAACGCAGAGTACATGGG30490.20195048801941481No Hit
GAATGGAATGGAATGGAATGGAATG28500.18876972478036477No Hit
CCCATGTACTCTGCGTTGATACCAC24940.16519006793060692No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA23420.15512234927565413No Hit
GTACATGGTAAGCAGTGGTATCAAC22180.14690921037292948No Hit
ACCATGTACTCTGCGTTGATACCAC21070.13955712635516793No Hit
GTATCAACGCAGAGTACTTTTTTTT20130.13333103718697342No Hit
GTGGTAAGCAGTGGTATCAACGCAG15430.102200591346001No Hit
GGTATCAACGCAGAGTACTTTTTTT15120.10014730662031984No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG500.001502253213.2967779
ACGAAAT604.0785607E-412.67157218
GTCTTGC2050.012.0658441
CTAGTAC951.0410749E-611.9986833
GTCCTAC600.00581395411.0990291
GACTATC600.00588323311.0813817
GTCTAGA1303.1974196E-810.9770621
GTCTTAG1502.3899702E-810.1476841
GGAACGA750.002651365510.13154915
TCTGTCG856.6074147E-410.0563868
GTCATAG1151.0089607E-59.9270821
GGACTGA1151.0282261E-59.9112986
CTTGCTC2800.09.4989573
TCAGTAC900.00111459329.4989573
TAGAACC900.00111493119.4986424
GACTGTT1201.7032808E-59.4983277
TCTGCAC2750.09.3262483
TTAGACA1651.067001E-79.2108044
ACAGGGC1651.0686381E-79.2098888
CTAGGGC1151.1115591E-49.0859583
Produced by FastQC (version 0.11.5)