FastQCFastQC Report
Wed 25 May 2016
SRR1294544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509776
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78620.5207395004292028No Hit
GGTATCAACGCAGAGTACTTTTTTT60410.4001255815432223No Hit
TATCAACGCAGAGTACTTTTTTTTT45640.30229649961318766No Hit
ATTCCATTCCATTCCATTCCATTCC36220.23990313794894075No Hit
GTGGTATCAACGCAGAGTACATGGG31300.20731552230264622No Hit
GAATGGAATGGAATGGAATGGAATG28090.18605409014317356No Hit
CCCATGTACTCTGCGTTGATACCAC27860.18453068534670045No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23880.15816915886860036No Hit
GTACATGGTAAGCAGTGGTATCAAC23560.15604963915176823No Hit
ACCATGTACTCTGCGTTGATACCAC22470.14883002511630866No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19830.13134398745244327No Hit
GTGGTAAGCAGTGGTATCAACGCAG17650.11690475938152414No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15710.10405517109822915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCAA500.001500912413.29835315
GTCCTAC1053.445828E-610.8622751
TATTCCG1002.3927632E-510.4521685
CTAGTCA856.5801473E-410.0609114
TAGACAG2054.1836756E-119.73372655
GCCCTAG800.0044977549.5044911
GTCTAGA1401.0511303E-69.504491
ACTAGAC1002.7489857E-49.5019713
CTTAGAC1106.818831E-59.5019713
CTGGGCG800.00451879.499149
AATCCGA900.00111473549.49882411
CGAGTAG900.00111473549.49882413
AATACTG2650.09.3226885
GTCTTGC2550.09.3181281
GTGCTAG1252.7375296E-59.12431051
AAGGGTT1151.1077235E-49.0888425
CTAATAC2304.1836756E-119.0888423
GGACTAT1054.481959E-49.0494976
ATACTGC2002.6411726E-99.0268736
CTACACT1901.0526492E-89.0018674
Produced by FastQC (version 0.11.5)