FastQCFastQC Report
Wed 25 May 2016
SRR1294543_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294543_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928380
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC42280.21925139236042687No Hit
GTACATGGTAAGCAGTGGTATCAAC39300.20379800661695308No Hit
ACCATGTACTCTGCGTTGATACCAC38530.19980501768323672No Hit
ATTCCATTCCATTCCATTCCATTCC31960.16573496924879952No Hit
GTGGTAAGCAGTGGTATCAACGCAG25810.13384291477820762No Hit
GAATGGAATGGAATGGAATGGAATG25810.13384291477820762No Hit
GTGGTATCAACGCAGAGTACATGGG25010.12969435484707373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA24540.12725707588753254No Hit
CATGTACTCTGCGTTGATACCACTG22640.11740424605108951No Hit
GGTAAGCAGTGGTATCAACGCAGAG21930.11372239911220816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGAT701.09242945E-412.21276814
AAGCGGT600.005891056311.07965810
CTCCCGC600.005892193411.0793711
GTCTTAC2054.0017767E-119.7418711
GTACAAG3200.09.5099221
GTCATAG1304.182746E-69.5099211
GTATTGG2201.6370905E-119.5099211
GTTAGCC2002.6557245E-109.498083
TATTCCG800.0045267189.4973425
CTTAGAC1856.719347E-99.2413753
GTATAAA4650.09.203151
GGAACGA1356.8578975E-69.14701215
CTAGTAC1356.8646714E-69.1462993
TAATACT3850.09.12778954
CTGTCGC1252.7640153E-59.117219
CGCCTCC1252.7657059E-59.11673811
GTATTAG2304.1836756E-119.0964471
TCGCTCT1151.1134164E-49.0848864
TAGGACT2304.1836756E-119.0848864
ACTGGCC1606.7695146E-78.9035278
Produced by FastQC (version 0.11.5)