FastQCFastQC Report
Wed 25 May 2016
SRR1294543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294543_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928380
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53040.2750495234341779No Hit
CCCATGTACTCTGCGTTGATACCAC44580.23117850216243688No Hit
GTACATGGTAAGCAGTGGTATCAAC40730.21121355749385493No Hit
ACCATGTACTCTGCGTTGATACCAC40180.20836142254120038No Hit
GGTATCAACGCAGAGTACTTTTTTT35580.18450720293718043No Hit
ATTCCATTCCATTCCATTCCATTCC31670.16423111627376347No Hit
TATCAACGCAGAGTACTTTTTTTTT30530.15831941837189764No Hit
GTGGTAAGCAGTGGTATCAACGCAG29380.15235586347089267No Hit
GAATGGAATGGAATGGAATGGAATG26650.1381989027058982No Hit
GTGGTATCAACGCAGAGTACATGGG24580.12746450388408923No Hit
GGTAAGCAGTGGTATCAACGCAGAG22730.11787095904334206No Hit
CATGTACTCTGCGTTGATACCACTG22710.11776724504506374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCG250.006034780818.9988087
GTCTCGC1001.4306897E-712.3581981
CAATCCG658.0344314E-411.69066310
CCCATAC1605.4205884E-1010.6898783
TCTCGCT1252.2321365E-710.642922
ATTAGAC1659.258656E-1010.3659433
GTCCTAG1752.3283064E-1010.3211321
GTAGCAC1409.658288E-810.1808373
GCTACAC1502.4365363E-810.1355883
GTAAGCC750.002643182210.1355883
TATTCCG1151.0238389E-59.9152495
GTGTTAG2500.09.8865591
TCGCTCT1356.42367E-79.8540454
GTCTAAT2002.6193447E-109.5063071
GTTACAC1502.6396083E-79.5021143
GTTAGCC1606.6120265E-89.5021133
CTACACT2950.09.3410624
TTAGACT2252.7284841E-119.29095654
ATAGAAC2252.7284841E-119.29095653
CTCGCTC1651.06187144E-79.2141713
Produced by FastQC (version 0.11.5)