FastQCFastQC Report
Wed 25 May 2016
SRR1294542_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294542_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808428
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC248610.8852283198999583No Hit
ACCATGTACTCTGCGTTGATACCAC149390.5319345911663037No Hit
GTACATGGTAAGCAGTGGTATCAAC147960.5268427746767943No Hit
GTGGTAAGCAGTGGTATCAACGCAG129360.46061355320485337No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA117610.41877520093091225No Hit
CATGTACTCTGCGTTGATACCACTG114020.40599224904466125No Hit
GGTAAGCAGTGGTATCAACGCAGAG100030.35617790450743264No Hit
GTATCAACGCAGAGTACATGGGAAG73410.261391782164257No Hit
GTGGTATCAACGCAGAGTACATGGG71370.25412793206733447No Hit
CCATGTACTCTGCGTTGATACCACT70430.25078086388541915No Hit
ACTCTGCGTTGATACCACTGCTTAC67600.24070405223135505No Hit
GCAGTGGTATCAACGCAGAGTACAT62140.22126257108959174No Hit
GTACATGGGAAGCAGTGGTAAGCAG61210.2179511100159947No Hit
GGTATCAACGCAGAGTACATGGGAA60570.21567225508362683No Hit
GAGTACATGGTAAGCAGTGGTATCA55570.19786870092450295No Hit
GTACTCTGCGTTGATACCACTGCTT54650.19459284695922416No Hit
GCTTACCACTGCTTCCCATGTACTC54450.1938807047928592No Hit
GCTTACCATGTACTCTGCGTTGATA53130.1891805664948505No Hit
GTATATGGTAAGCAGTGGTATCAAC51040.1817386808563367No Hit
TATCAACGCAGAGTACATGGGAAGC46240.16464726886357778No Hit
GCGTTGATACCACTGCTTACCATGT44530.1585584533411574No Hit
ATGGTAAGCAGTGGTATCAACGCAG43390.15449924299287715No Hit
GCGTTGATACCACTGCTTACCACTG41210.14673689337949913No Hit
AAGCAGTGGTATCAACGCAGAGTAC40670.14481410953031376No Hit
GTACATGGGAAGCAGTGGTATCAAC40070.1426776830312189No Hit
CTGCTTACCATGTACTCTGCGTTGA36930.1314970510192891No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA36550.13014398090319565No Hit
GTATCAACGCAGAGTACATGGTAAG36210.12893333922037525No Hit
CAGTGGTATCAACGCAGAGTACATG35890.1277939117541913No Hit
GTACATGATAAGCAGTGGTATCAAC35520.12647644874641614No Hit
GTGATAAGCAGTGGTATCAACGCAG35380.1259779492299607No Hit
GTACATAAGCAGTGGTATCAACGCA33400.11892774178294761No Hit
ATTCCATTCCATTCCATTCCATTCC33300.11857167069976512No Hit
ATCATGTACTCTGCGTTGATACCAC32880.11707617215039871No Hit
ATGCTAAGCAGTGGTATCAACGCAG30840.10981232205347619No Hit
ACCATATACTCTGCGTTGATACCAC29320.10440004158910252No Hit
ACGCAGAGTACATGGTAAGCAGTGG28850.10272650749814487No Hit
TATCAACGCAGAGTACATGGTAAGC28110.10009158148259455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCGC551.9644095E-413.81538410
TCGCCTA500.00150236413.29730713
TAGACGG655.465919E-513.1511825
GTTCGCC550.00307416412.08846111
TACACCG1052.7298483E-711.7595235
AGGACCG658.0406066E-411.68993955
TAAGAGT1657.2759576E-1211.513234
ACGACGG752.067879E-411.40419119
TATGGTA11950.011.2868194
CCGAATT855.332334E-511.17520214
GTACTAA1553.0559022E-1011.0471411
TACGACG803.758686E-410.6918118
TAGACCG803.779782E-410.6853365
GACCGCG1252.2478889E-710.6380357
TATATGG13250.010.6112512
GTATATG17100.010.5697961
CGCTCCG1002.4056348E-510.4478845
CGCTTAA750.002619250410.1470041
TAGGACT1502.4536348E-810.1316424
TACATCG750.00265316110.1312825
Produced by FastQC (version 0.11.5)