FastQCFastQC Report
Wed 25 May 2016
SRR1294542_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294542_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808428
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC262990.9364313416615986No Hit
ACCATGTACTCTGCGTTGATACCAC154790.5511624296581575No Hit
GTACATGGTAAGCAGTGGTATCAAC153610.5469607908766043No Hit
GTGGTAAGCAGTGGTATCAACGCAG142420.507116436668485No Hit
CATGTACTCTGCGTTGATACCACTG120270.42824669174356617No Hit
GGTAAGCAGTGGTATCAACGCAGAG109100.38847355175208337No Hit
GTATCAACGCAGAGTACTTTTTTTT91650.326339147736741No Hit
GTATCAACGCAGAGTACATGGGAAG77720.2767384458494218No Hit
GTGGTATCAACGCAGAGTACATGGG74490.26523734986262776No Hit
CCATGTACTCTGCGTTGATACCACT73490.26167663903080296No Hit
ACTCTGCGTTGATACCACTGCTTAC70960.2526680406262863No Hit
GCAGTGGTATCAACGCAGAGTACAT64970.2313393827436559No Hit
GGTATCAACGCAGAGTACATGGGAA64790.2306984547939274No Hit
GTACATGGGAAGCAGTGGTAAGCAG64270.22884688516137852No Hit
GGTATCAACGCAGAGTACTTTTTTT61120.21763064604113047No Hit
TATCAACGCAGAGTACTTTTTTTTT59160.2106516528107539No Hit
GAGTACATGGTAAGCAGTGGTATCA58890.20969026088616122No Hit
GTACTCTGCGTTGATACCACTGCTT57710.205488622104608No Hit
GCTTACCATGTACTCTGCGTTGATA55370.197156558758138No Hit
GCTTACCACTGCTTCCCATGTACTC54900.19548302466718034No Hit
GTATATGGTAAGCAGTGGTATCAAC53140.18921617360316875No Hit
TATCAACGCAGAGTACATGGGAAGC48820.17383390280968572No Hit
ATGGTAAGCAGTGGTATCAACGCAG46940.16713976644585513No Hit
AAGCAGTGGTATCAACGCAGAGTAC46700.16628519584621718No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA45890.16340102007243912No Hit
GCGTTGATACCACTGCTTACCATGT44660.1590213457492946No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43230.1539295292597852No Hit
GCGTTGATACCACTGCTTACCACTG42850.15257645914369178No Hit
GTACATGGGAAGCAGTGGTATCAAC41640.1482679990371838No Hit
CAGTGGTATCAACGCAGAGTACATG40150.1429625398977649No Hit
CTGCTTACCATGTACTCTGCGTTGA38960.13872529400789338No Hit
GTGATAAGCAGTGGTATCAACGCAG38860.1383692229247109No Hit
GTATCAACGCAGAGTACATGGTAAG38110.13569868980084232No Hit
GTACATGATAAGCAGTGGTATCAAC36860.13124780126106134No Hit
ATCATGTACTCTGCGTTGATACCAC36260.12911137476196646No Hit
ATTCCATTCCATTCCATTCCATTCC34060.12127781093195196No Hit
ATGCTAAGCAGTGGTATCAACGCAG32610.11611478022580603No Hit
GTACATAAGCAGTGGTATCAACGCA32330.11511778119289509No Hit
ACCATATACTCTGCGTTGATACCAC30670.10920700121206596No Hit
TATCAACGCAGAGTACATGGTAAGC30570.10885093012888349No Hit
ACGCAGAGTACATGGTAAGCAGTGG30500.10860168037065575No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30350.10806757374588205No Hit
ACGCAGAGTACATGGGAAGCAGTGG29720.10582432592183243No Hit
GAGTACATGGGAAGCAGTGGTAAGC28960.10311818568964559No Hit
GCTTCCCATGTACTCTGCGTTGATA28310.10080372364895948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCT500.0015021313.29758316
TGGACCG1459.640644E-1111.7951595
AGTTCGC752.0763969E-411.39914510
GTTCTAG1803.6379788E-1211.089611
AAAGCGT600.005870038611.0852656
CTGTCCG600.005880162611.08279
TACGACG700.001494316410.85516918
GTCTTAG2150.010.6106581
ACTGATC2053.6379788E-1210.1945398
TGTTAGG1502.440538E-810.1350992
TCGCCTA750.002650748710.13239313
GACCGCG1054.10839E-59.9521667
CTAAGCT1451.6143713E-79.82934
TTAGGGT1951.6370905E-109.7452894
GTCCTAT900.00110834819.5053811
GCACCGT1304.231866E-69.5016576
CGAAGCA800.00451087359.5016574
ACCGCGG1401.0601325E-69.4994568
GTTCGCC900.00111486749.49928811
GTATTAT3050.09.3495551
Produced by FastQC (version 0.11.5)