FastQCFastQC Report
Wed 25 May 2016
SRR1294541_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294541_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431704
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC100290.41242684142477865No Hit
GTACATGGTAAGCAGTGGTATCAAC76410.3142240996437066No Hit
ACCATGTACTCTGCGTTGATACCAC75250.3094537822037551No Hit
GTGGTAAGCAGTGGTATCAACGCAG57710.23732329263759078No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA53220.2188588742708817No Hit
CATGTACTCTGCGTTGATACCACTG51310.21100429986544417No Hit
GGTAAGCAGTGGTATCAACGCAGAG43330.17818780575267384No Hit
GTGGTATCAACGCAGAGTACATGGG42200.17354085859134172No Hit
ACTCTGCGTTGATACCACTGCTTAC34350.14125896901925564No Hit
CCATGTACTCTGCGTTGATACCACT33980.1397374022496159No Hit
GTATCAACGCAGAGTACATGGGAAG32240.13258192608968855No Hit
GCTTACCATGTACTCTGCGTTGATA28690.11798310978638848No Hit
GAGTACATGGTAAGCAGTGGTATCA28000.11514559337814143No Hit
GCAGTGGTATCAACGCAGAGTACAT27770.11419975457539241No Hit
GGTATCAACGCAGAGTACATGGGAA27270.11214358326506846No Hit
GTACTCTGCGTTGATACCACTGCTT26980.11095100390508056No Hit
GTACATGGGAAGCAGTGGTAAGCAG26370.10844247490648534No Hit
GCTTACCACTGCTTCCCATGTACTC24560.10099913476311262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT755.8691512E-815.1959684
TCCGTAC400.005290256314.2459283
TAGTACG1450.013.1040162
TACGGTC658.0427685E-411.6894485
AGTACGG2050.011.1187733
TAGGACG600.00587538111.0838132
TTCCGTA700.001491387510.8576132
ACTGTCG700.001494974710.8544868
TACGGGC803.7808606E-410.6848865
TTAGACA2550.010.0561554
ACACCGA951.6487544E-49.9975536
TCGTGGG1252.5765712E-69.87839512
ATTAGAC1554.083813E-89.8036493
GTGTTAA3300.09.5107811
GACAGTA1804.2000465E-99.4978717
GGCGTGC1201.7060245E-59.4976758
TCTATAC2700.09.4972853
GTCCTAG1701.6565536E-78.9513241
CTAACAC2401.0186341E-108.7058443
CATAAGC6600.08.6340734
Produced by FastQC (version 0.11.5)