FastQCFastQC Report
Wed 25 May 2016
SRR1294541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294541_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431704
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC106370.43742988455831794No Hit
GTACATGGTAAGCAGTGGTATCAAC81960.3370476011883025No Hit
ACCATGTACTCTGCGTTGATACCAC76350.3139773590864678No Hit
GTATCAACGCAGAGTACTTTTTTTT69010.2837927642509121No Hit
GTGGTAAGCAGTGGTATCAACGCAG66560.27371752483032474No Hit
CATGTACTCTGCGTTGATACCACTG54130.22260110605567124No Hit
GGTAAGCAGTGGTATCAACGCAGAG49100.20191602267381228No Hit
GGTATCAACGCAGAGTACTTTTTTT47740.19632323670973115No Hit
GTGGTATCAACGCAGAGTACATGGG43130.17736533722854428No Hit
TATCAACGCAGAGTACTTTTTTTTT39760.16350674259696082No Hit
CCATGTACTCTGCGTTGATACCACT35030.14405536200129623No Hit
ACTCTGCGTTGATACCACTGCTTAC34740.1428627826413083No Hit
GTATCAACGCAGAGTACATGGGAAG33420.13743449038205308No Hit
GGTATCAACGCAGAGTACATGGGAA29840.12271230380013357No Hit
GAGTACATGGTAAGCAGTGGTATCA29840.12271230380013357No Hit
GCAGTGGTATCAACGCAGAGTACAT29310.12053276221119019No Hit
GCTTACCATGTACTCTGCGTTGATA28360.11662603672157466No Hit
GTACATGGGAAGCAGTGGTAAGCAG27530.11321279234643691No Hit
GTACTCTGCGTTGATACCACTGCTT27180.11177347242921014No Hit
GCTTACCACTGCTTCCCATGTACTC25210.10367215746653377No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25080.10313755292584953No Hit
GTACATGATAAGCAGTGGTATCAAC24410.10038228337001544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAC802.001918E-613.0618078
GGCGCGA550.003074780312.08803115
TTCCGTA1053.467021E-610.8574597
GTCTAGA1855.456968E-1210.7912021
ACCTGCG803.771307E-410.6878137
TTAGACA2250.010.5591224
GTACTAA1554.022877E-89.8131981
GTATTAG3300.09.5065351
GTTCCGG900.00110700499.5065351
TCTAGAC1901.0459189E-99.5030153
ACTAGAC2002.6375346E-109.5030153
CTGGTCG1201.70216E-59.49949559
TAGCACT2850.09.0030414
GTCTTAG1701.6679223E-78.9473281
TCGAGTG850.0074555898.93849719
GTTCTAG2451.6370905E-118.9245011
GTCTTAA2151.027729E-98.8432881
GTCCTAG2054.090907E-98.8109351
GACCACG1304.3684988E-58.7694877
ATACTGT5650.08.7461376
Produced by FastQC (version 0.11.5)