FastQCFastQC Report
Wed 25 May 2016
SRR1294540_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294540_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025089
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC42230.20853404467655495No Hit
GTACATGGTAAGCAGTGGTATCAAC33110.16349898695810408No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA32200.15900535729540777No Hit
ACCATGTACTCTGCGTTGATACCAC31090.15352411671783314No Hit
GTGGTATCAACGCAGAGTACATGGG25910.12794499402248494No Hit
GTGGTAAGCAGTGGTATCAACGCAG22700.11209383883868808No Hit
CATGTACTCTGCGTTGATACCACTG21590.10661259826111347No Hit
ATTCCATTCCATTCCATTCCATTCC20780.10261277405585632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTA400.005275780314.2516492
TCGTGGG400.00528616714.24742512
AACACCG550.00307485912.0878295
GTCTCGT550.003075427812.087539
CTAGGAC2050.011.5835583
CACCGCT752.0797987E-411.3968137
ACCACCG855.3383075E-511.1736245
GTTCTAA2500.010.6557121
TAGGACA2250.010.5536474
TATTCCG750.002653767410.1307525
CCTACAC2107.2759576E-129.9508293
GTATTAT4550.09.8276781
GTGTTAA3150.09.3630111
GTATTAC3150.09.3630111
GTCTAGT1356.7207347E-69.1616571
GGACAGC1356.8677364E-69.1460436
GTATAGG2201.6370905E-109.0815731
CTAGCAC2002.6630005E-99.0235913
TATACTG3900.09.0105245
CTAGCCT2151.0440999E-98.8356124
Produced by FastQC (version 0.11.5)