FastQCFastQC Report
Wed 25 May 2016
SRR1294540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294540_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025089
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58060.2867034485891731No Hit
CCCATGTACTCTGCGTTGATACCAC44630.2203853756550947No Hit
GGTATCAACGCAGAGTACTTTTTTT42760.2111512136009825No Hit
GTACATGGTAAGCAGTGGTATCAAC34960.1726343879207284No Hit
TATCAACGCAGAGTACTTTTTTTTT33310.16448659787298237No Hit
ACCATGTACTCTGCGTTGATACCAC32960.16275827877194532No Hit
GTGGTATCAACGCAGAGTACATGGG27390.13525331479258443No Hit
GTGGTAAGCAGTGGTATCAACGCAG26970.13317933187133998No Hit
CATGTACTCTGCGTTGATACCACTG23190.11451348558013993No Hit
ATTCCATTCCATTCCATTCCATTCC21610.1067113593526013No Hit
GGTAAGCAGTGGTATCAACGCAGAG21290.10513118188879599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGCC550.003072315412.08916611
CGCCACC1252.2468157E-710.6379414
GTCCTAG2350.010.5179531
CTGTCGC1106.052045E-610.3629089
TATTCCG750.002643198710.1356265
CCTAGAC1752.562956E-99.7736393
CTAGGAC2054.1836756E-119.7339093
GTATTAG3250.09.6528661
GTCTAGT1701.6460035E-89.5066111
TAGACTG3000.09.5021495
TGGGCGA800.00452090479.49886310
ACTGACC1951.6843842E-99.2557618
GTACTAA1856.6174835E-99.2496751
GCTACAC1356.8281115E-69.1502173
CGCGATC950.00182717138.99825817
GTCTCGC850.00739407358.9473981
TAAGGGT1701.6790182E-78.9431984
GGAACGA850.0074482068.93944515
GACCTGT1401.08202275E-58.8234246
TAGGGTG2054.123649E-98.80686955
Produced by FastQC (version 0.11.5)