FastQCFastQC Report
Wed 25 May 2016
SRR1294539_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294539_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284580
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC513582.2480280839366538No Hit
ACCATGTACTCTGCGTTGATACCAC358021.5671151809085258No Hit
GTACATGGTAAGCAGTGGTATCAAC334351.4635075156046187No Hit
GTGGTAAGCAGTGGTATCAACGCAG265781.1633648197918216No Hit
CATGTACTCTGCGTTGATACCACTG256601.1231823792557056No Hit
GGTAAGCAGTGGTATCAACGCAGAG215700.9441560374335763No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA191660.838928818426144No Hit
GTATCAACGCAGAGTACATGGGAAG165250.7233277013718058No Hit
CCATGTACTCTGCGTTGATACCACT164320.7192569312521339No Hit
ACTCTGCGTTGATACCACTGCTTAC154690.6771047632387572No Hit
GTACTCTGCGTTGATACCACTGCTT146530.6413870383177652No Hit
GGTATCAACGCAGAGTACATGGGAA134580.5890798308660673No Hit
GCAGTGGTATCAACGCAGAGTACAT130710.5721401745616262No Hit
GTACATGGGAAGCAGTGGTAAGCAG128250.5613723310192683No Hit
GAGTACATGGTAAGCAGTGGTATCA120260.5263987253674637No Hit
GCTTACCATGTACTCTGCGTTGATA119200.521758922865472No Hit
GTACATGATAAGCAGTGGTATCAAC115880.5072267112554606No Hit
GTGGTATCAACGCAGAGTACATGGG112340.4917315217676772No Hit
GCGTTGATACCACTGCTTACCATGT110490.4836337532500503No Hit
TATCAACGCAGAGTACATGGGAAGC109910.48109499339047No Hit
GTACATGGGAAGCAGTGGTATCAAC109310.47846869008745585No Hit
ATCATGTACTCTGCGTTGATACCAC108140.4733473986465783No Hit
GCTTACCACTGCTTCCCATGTACTC107450.47032714984811214No Hit
GCGTTGATACCACTGCTTACCACTG105010.459646849749188No Hit
CTGCTTACCATGTACTCTGCGTTGA97300.4258988523054566No Hit
AAGCAGTGGTATCAACGCAGAGTAC94530.4137740853898747No Hit
GTACATAAGCAGTGGTATCAACGCA85820.37564891577445303No Hit
GTATCAACGCAGAGTACATGGTAAG84860.37144683048963045No Hit
ATGGTAAGCAGTGGTATCAACGCAG83670.3662379956053191No Hit
CAGTGGTATCAACGCAGAGTACATG80640.3529751639250978No Hit
TATCAACGCAGAGTACATGGTAAGC72650.31800155827329313No Hit
ACGCAGAGTACATGGTAAGCAGTGG70660.3092909856516296No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA68220.29861068555270553No Hit
ACGCAGAGTACATGGGAAGCAGTGG64810.28368452844724196No Hit
GGTATCAACGCAGAGTACATGGTAA60280.26385593850948535No Hit
GTGATAAGCAGTGGTATCAACGCAG57760.25282546463682604No Hit
TACCACTGCTTACCATGTACTCTGC54940.24048183911265963No Hit
GAGTACATGGGAAGCAGTGGTAAGC51110.2237172696950862No Hit
GTGGTATCAACGCAGAGTACATGGT50140.2194714126885467No Hit
GCTTATGTACTCTGCGTTGATACCA49910.21846466308905796No Hit
GCTTCCCATGTACTCTGCGTTGATA48850.21382486058706632No Hit
GAGTACATGGGAAGCAGTGGTATCA46190.2021815826103704No Hit
AAAAAGTACTCTGCGTTGATACCAC45680.1999492248028084No Hit
ACTCTGCGTTGATACCACTGCTTAT44340.19408381409274353No Hit
GATAAGCAGTGGTATCAACGCAGAG44280.19382118376244212No Hit
GAGTACATGATAAGCAGTGGTATCA42490.18598604557511664No Hit
GCAGAGTACATGGTAAGCAGTGGTA39250.1718040077388404No Hit
GCTTATCATGTACTCTGCGTTGATA38890.17022822575703192No Hit
AAAGTACTCTGCGTTGATACCACTG38460.16834604172320514No Hit
TGGTAAGCAGTGGTATCAACGCAGA37420.16379378266464734No Hit
GTAAGCAGTGGTATCAACGCAGAGT35560.15565224242530357No Hit
CTTCCCATGTACTCTGCGTTGATAC35030.15333234117430775No Hit
GTACATGGTAAGCAGTGGTAAGCAG34640.15162524402734856No Hit
CTGCTTACCACTGCTTCCCATGTAC34280.1500494620455401No Hit
GCGTTGATACCACTGCTTCCCATGT33490.14659149602990484No Hit
GCGTTGATACCACTGCTTATCATGT32810.1436150189531555No Hit
GTATCAACGCAGAGTACATGGGAAA31280.1369179455304695No Hit
CTTATGTACTCTGCGTTGATACCAC30740.1345542725577568No Hit
GTATCAACGCAGAGTACTTTTTTTT30520.13359129467998493No Hit
GATACCACTGCTTACCATGTACTCT29990.13127139342898914No Hit
GTTGATACCACTGCTTACCATGTAC29960.13114007826383844No Hit
CTGCTTCCCATGTACTCTGCGTTGA29950.13109630654212154No Hit
GAGTAAGCAGTGGTATCAACGCAGA29930.13100876309868772No Hit
GCTTACCACTGCTTACCATGTACTC28810.1261063302663947No Hit
ATGATAAGCAGTGGTATCAACGCAG27350.11971565889572701No Hit
ATACCACTGCTTACCATGTACTCTG26730.11700181214927907No Hit
GTACATGGGAAGCAGTGATAAGCAG26340.1152947150023199No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT25720.11258086825587196No Hit
CCACTGCTTACCATGTACTCTGCGT25330.11087377110891279No Hit
GTTGATACCACTGCTTACCACTGCT25250.1105235973351776No Hit
TTCCCATGTACTCTGCGTTGATACC25100.10986702150942405No Hit
CATGGTAAGCAGTGGTATCAACGCA23980.10496458867713103No Hit
ATCAACGCAGAGTACATGGGAAGCA23610.10334503497360566No Hit
TCCATGTACTCTGCGTTGATACCAC23410.10246960053926762No Hit
GTATCAACGCAGAGTACATGATAAG23370.1022945136524No Hit
GTACATGGGAAGCAATGGTAAGCAG22980.10058741650544083No Hit
GCAGTGGTAAGCAGTGGTATCAACG22880.1001496992882718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACT851.024091E-915.6433094
CTATACT755.8667865E-815.1963574
GAACAAA8350.013.2144021
GTATTAG1051.960143E-812.6827881
ACTATAC1001.4480793E-712.3470393
TTAGAAC1001.4480793E-712.3470393
CTAGACA550.00307471912.0880114
TAAGACT658.0421066E-411.6895074
TATTAGA907.45555E-611.6123962
ATCTAAG752.0724979E-411.401262
AACGTTG1751.8189894E-1211.39751712
TAGGACC752.0792522E-411.3972674
AAAAGTA9450.011.3610462
CTCGATA600.005886625511.0809212
GTCTTAC700.001476119510.8709611
GACTAGA700.001476119510.8709611
GCAAAAA7250.010.7585031
GTACAGG2500.010.6535421
TAACCTG1252.2487802E-710.637455
TTAAATC909.541799E-510.5530263
Produced by FastQC (version 0.11.5)