FastQCFastQC Report
Wed 25 May 2016
SRR1294539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294539_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284580
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC539112.3597772894799043No Hit
ACCATGTACTCTGCGTTGATACCAC367861.6101865550779575No Hit
GTACATGGTAAGCAGTGGTATCAAC352591.543347136016248No Hit
GTGGTAAGCAGTGGTATCAACGCAG303311.327640091395355No Hit
CATGTACTCTGCGTTGATACCACTG271421.1880520708401545No Hit
GGTAAGCAGTGGTATCAACGCAGAG240511.0527536790132102No Hit
CCATGTACTCTGCGTTGATACCACT174520.7639040874033739No Hit
GTATCAACGCAGAGTACATGGGAAG173680.7602272627791541No Hit
ACTCTGCGTTGATACCACTGCTTAC160160.7010478950179027No Hit
GTACTCTGCGTTGATACCACTGCTT145360.6362657468768876No Hit
GCAGTGGTATCAACGCAGAGTACAT142220.6225214262577804No Hit
GGTATCAACGCAGAGTACATGGGAA141980.6214709049365749No Hit
GTACATGGGAAGCAGTGGTAAGCAG134730.5897364066918208No Hit
GAGTACATGGTAAGCAGTGGTATCA127880.5597527773157429No Hit
GCTTACCATGTACTCTGCGTTGATA122690.5370352537446708No Hit
GTATCAACGCAGAGTACTTTTTTTT119600.523509791734148No Hit
TATCAACGCAGAGTACATGGGAAGC119060.5211461187614354No Hit
GTACATGATAAGCAGTGGTATCAAC117910.5161123707639916No Hit
GTGGTATCAACGCAGAGTACATGGG117340.5136173826261282No Hit
ATCATGTACTCTGCGTTGATACCAC113470.49667772632168716No Hit
AAGCAGTGGTATCAACGCAGAGTAC112960.4944453685141252No Hit
GCGTTGATACCACTGCTTACCATGT110970.48573479589246166No Hit
GTACATGGGAAGCAGTGGTATCAAC110480.48358998152833343No Hit
GCTTACCACTGCTTCCCATGTACTC109170.4778558859834193No Hit
GCGTTGATACCACTGCTTACCACTG106070.46428665225117965No Hit
CTGCTTACCATGTACTCTGCGTTGA99430.43522222903115676No Hit
ATGGTAAGCAGTGGTATCAACGCAG94150.4121107599646324No Hit
GTATCAACGCAGAGTACATGGTAAG89270.3907501597667843No Hit
GTACATAAGCAGTGGTATCAACGCA87420.3826523912491574No Hit
CAGTGGTATCAACGCAGAGTACATG85710.37516742683556714No Hit
GTACTTTTTTTTTTTTTTTTTTTTT84780.37109665671589526No Hit
TATCAACGCAGAGTACTTTTTTTTT80080.35052394750895133No Hit
ACGCAGAGTACATGGTAAGCAGTGG76740.33590419245550607No Hit
TATCAACGCAGAGTACATGGTAAGC76180.3334529760393595No Hit
GGTATCAACGCAGAGTACTTTTTTT75770.33165833544896656No Hit
ACGCAGAGTACATGGGAAGCAGTGG70510.3086344098258761No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA65100.2849539083770321No Hit
GTGATAAGCAGTGGTATCAACGCAG63040.27593693370335026No Hit
GGTATCAACGCAGAGTACATGGTAA62560.273835891060939No Hit
TACCACTGCTTACCATGTACTCTGC55530.24306437069395687No Hit
GAGTACATGGGAAGCAGTGGTAAGC54150.2370238730970244No Hit
GTGGTATCAACGCAGAGTACATGGT53390.23369722224653985No Hit
GCTTATGTACTCTGCGTTGATACCA52010.22765672464960737No Hit
GCTTCCCATGTACTCTGCGTTGATA50480.22095965122692135No Hit
GATAAGCAGTGGTATCAACGCAGAG50230.21986535818399883No Hit
AAAAAGTACTCTGCGTTGATACCAC48360.21168004622293812No Hit
GAGTACATGGGAAGCAGTGGTATCA47600.2083533953724536No Hit
GAGTACTTTTTTTTTTTTTTTTTTT47280.20695270027751272No Hit
ACTCTGCGTTGATACCACTGCTTAT47060.20598972239974084No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44570.19509056369223227No Hit
GAGTACATGATAAGCAGTGGTATCA43850.19193899972861533No Hit
GCAGAGTACATGGTAAGCAGTGGTA42090.18423517670644057No Hit
GTAAGCAGTGGTATCAACGCAGAGT41710.1825718512811983No Hit
GCTTATCATGTACTCTGCGTTGATA40700.17815090738779119No Hit
TGGTAAGCAGTGGTATCAACGCAGA39260.17184777946055732No Hit
AAAGTACTCTGCGTTGATACCACTG38250.1674268355671502No Hit
CTTCCCATGTACTCTGCGTTGATAC38020.16642008596766147No Hit
GTACATGGTAAGCAGTGGTAAGCAG36450.15954792565810783No Hit
CTGCTTACCACTGCTTCCCATGTAC35610.15587110103388807No Hit
GCGTTGATACCACTGCTTCCCATGT35040.15337611289602465No Hit
GTATCAACGCAGAGTACATGGGAAA34470.15088112475816123No Hit
GTGGTATCAACGCAGAGTACTTTTT34310.1501807772106908No Hit
GCGTTGATACCACTGCTTATCATGT33650.14729184357737526No Hit
CTTATGTACTCTGCGTTGATACCAC33090.14484062716122875No Hit
GAGTAAGCAGTGGTATCAACGCAGA32750.1433523886228541No Hit
CTGCTTCCCATGTACTCTGCGTTGA31150.13634891314814976No Hit
GTTGATACCACTGCTTACCATGTAC30890.1352108483835103No Hit
GATACCACTGCTTACCATGTACTCT30760.1346418160011906No Hit
ATGATAAGCAGTGGTATCAACGCAG30610.13398524017543706No Hit
GCTTACCACTGCTTACCATGTACTC29400.1286888618476919No Hit
ATACCACTGCTTACCATGTACTCTG28460.12457432000630314No Hit
CATGGTAAGCAGTGGTATCAACGCA27370.11980320233916081No Hit
ATCAACGCAGAGTACATGGGAAGCA27310.11954057200885938No Hit
CCACTGCTTACCATGTACTCTGCGT27010.11822742035735234No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT27000.11818364863563544No Hit
AAGCAGTGGTAAGCAGTGGTATCAA26630.11656409493211006No Hit
GCAGTGGTAAGCAGTGGTATCAACG26570.11630146460180864No Hit
GTTGATACCACTGCTTACCACTGCT26080.11415665023768046No Hit
GCTTATCACTGCTTCCCATGTACTC25540.11179297726496774No Hit
GTACATGGGAAGCAGTGATAAGCAG25410.11122394488264802No Hit
TTCCCATGTACTCTGCGTTGATACC24700.10811615264074796No Hit
GCAGTGGTATCAACGCAGAGTACTT24440.10697808787610852No Hit
CCACTGCTTCCCATGTACTCTGCGT24250.10614642516348738No Hit
ACTCTGCGTTGATACCACTGCTTCC24110.10553362105945076No Hit
GTACATGGGAAGCAATGGTAAGCAG24100.10548984933773384No Hit
ACATGGTAAGCAGTGGTATCAACGC24080.10540230589430005No Hit
TGATACCACTGCTTACCATGTACTC23480.10277600259128593No Hit
TCCATGTACTCTGCGTTGATACCAC23420.10251337226098452No Hit
GTATCAACGCAGAGTACATGATAAG23400.1024258288175507No Hit
TACCACTGCTTCCCATGTACTCTGC23060.10093759027917605No Hit
TATGTACTCTGCGTTGATACCACTG23010.10071873167059153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAC400.005270750714.2538113
GCCGAAT400.005284548314.24819512
TGTACTG903.628884E-813.7258915
CGCCCTA500.00150234813.29715116
TAGGATA604.0841138E-412.67005354
GAACAAA4000.012.5961061
TAGAACT550.003063065212.0941424
CTAGTGT550.003063065212.0941424
GCGGTAG550.00307312612.08884815
ATTGGAC658.0079725E-411.6954353
GTACTAA2100.011.3172561
GTATTAG1104.9277696E-711.2349481
GTAAGAC855.310685E-511.1794593
AAAAAGT9050.011.1346791
AAAAGTA9250.011.0951012
CCTTAAG1201.264143E-711.0909111
TCCTTAC600.005847268711.0909111
AGCCGAA600.005882637611.08192911
CGGTAGG600.005886472311.08095916
ATAGGAC700.001488544210.8600453
Produced by FastQC (version 0.11.5)