FastQCFastQC Report
Wed 25 May 2016
SRR1294538_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294538_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134594
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC285841.3390836852347565No Hit
ACCATGTACTCTGCGTTGATACCAC215931.0115740979315038No Hit
GTACATGGTAAGCAGTGGTATCAAC214001.0025325659118316No Hit
GTGGTAAGCAGTGGTATCAACGCAG147290.6900141197810918No Hit
CATGTACTCTGCGTTGATACCACTG145320.6807851984967633No Hit
GGTAAGCAGTGGTATCAACGCAGAG128900.603861905355304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA111970.5245493990894755No Hit
CCATGTACTCTGCGTTGATACCACT98900.46331995686299127No Hit
GTATCAACGCAGAGTACATGGGAAG92800.4347430940028877No Hit
ACTCTGCGTTGATACCACTGCTTAC91080.4266853556226617No Hit
GTACATGGGAAGCAGTGGTAAGCAG81080.3798380394585575No Hit
GTACTCTGCGTTGATACCACTGCTT80800.37852631460596253No Hit
GAGTACATGGTAAGCAGTGGTATCA74970.35121432928228974No Hit
GCAGTGGTATCAACGCAGAGTACAT74170.3474665439891614No Hit
GGTATCAACGCAGAGTACATGGGAA71970.3371601344330585No Hit
GCTTACCATGTACTCTGCGTTGATA71690.3358484095804636No Hit
GCTTACCACTGCTTCCCATGTACTC70390.32975825847913No Hit
GCGTTGATACCACTGCTTACCATGT69570.32591677855367346No Hit
GTGGTATCAACGCAGAGTACATGGG66910.3134553924540217No Hit
TATCAACGCAGAGTACATGGGAAGC60630.2840352779029642No Hit
GTACATGATAAGCAGTGGTATCAAC58850.2756964556257537No Hit
GCGTTGATACCACTGCTTACCACTG58720.2750874405156203No Hit
CTGCTTACCATGTACTCTGCGTTGA58490.2740099522438459No Hit
GTACATGGGAAGCAGTGGTATCAAC58210.27269822739125094No Hit
GTATCAACGCAGAGTACATGGTAAG53400.2501646683163168No Hit
ATCATGTACTCTGCGTTGATACCAC52390.2454330893837423No Hit
AAGCAGTGGTATCAACGCAGAGTAC52060.24388712795032685No Hit
ATGGTAAGCAGTGGTATCAACGCAG47490.22247790446333118No Hit
CAGTGGTATCAACGCAGAGTACATG44670.2092669613050538No Hit
ACGCAGAGTACATGGTAAGCAGTGG43360.20312996288755614No Hit
TATCAACGCAGAGTACATGGTAAGC43320.20294257362289972No Hit
GTACATAAGCAGTGGTATCAACGCA42790.2004596658662022No Hit
GTGATAAGCAGTGGTATCAACGCAG36900.17286659664554477No Hit
ACGCAGAGTACATGGGAAGCAGTGG35950.16841610160995488No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA34310.16073314175904177No Hit
GAGTACATGGGAAGCAGTGGTAAGC33520.15703220378207752No Hit
GGTATCAACGCAGAGTACATGGTAA33330.15614210477495954No Hit
TACCACTGCTTACCATGTACTCTGC32290.1512699838938927No Hit
GTATCAACGCAGAGTACTTTTTTTT30520.14297800893284623No Hit
GTGGTATCAACGCAGAGTACATGGT28580.13388962959701003No Hit
GAGTAAGCAGTGGTATCAACGCAGA27260.12770578386334827No Hit
AAAAAGTACTCTGCGTTGATACCAC26760.12536341805514303No Hit
GCTTCCCATGTACTCTGCGTTGATA25970.1216624800781788No Hit
GCTTATGTACTCTGCGTTGATACCA25440.11917957232148127No Hit
GCAGAGTACATGGTAAGCAGTGGTA23560.11037227688262968No Hit
GAGTACATGGGAAGCAGTGGTATCA23150.10845153691990139No Hit
GATAAGCAGTGGTATCAACGCAGAG22910.1073272013319629No Hit
TGGTAAGCAGTGGTATCAACGCAGA22810.10685872817032184No Hit
GTAAGCAGTGGTATCAACGCAGAGT22680.10624971306018849No Hit
ACTCTGCGTTGATACCACTGCTTAT21990.1030172482448653No Hit
GTACATGGTAAGCAGTGGTAAGCAG21940.10278301166404477No Hit
CTGCTTACCACTGCTTCCCATGTAC21810.10217399655391142No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG456.772652E-414.7752275
GTCATAG751.4640707E-512.6793341
ACTATCC604.0991037E-412.664483
GTAAGAC1051.999797E-812.664483
GCGAAAA1353.6561687E-1011.9749281
CTAGATT1106.0582515E-610.3620894
TGGACAG750.0026520610.1315835
TCTGTCG1151.0293967E-59.9108678
CTAACCT1252.5755835E-69.8785264
GTCTTAG1554.0021405E-89.8162581
AGTACTG1651.0251824E-89.7861895
GAACAAA7000.09.7812011
CATAAGC9300.09.7028534
GTATTAT3350.09.6514331
GTTCTAA1606.535083E-89.5095011
GCGTTAA800.0044795229.5095011
TATACTC4650.09.3962275
TAATACT3250.09.352454
GTACATA13450.09.3327451
CTAGGAC1451.7105504E-69.170833
Produced by FastQC (version 0.11.5)