FastQCFastQC Report
Wed 25 May 2016
SRR1294538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294538_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134594
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC302261.416006978376216No Hit
ACCATGTACTCTGCGTTGATACCAC227091.063855702770644No Hit
GTACATGGTAAGCAGTGGTATCAAC218701.0245508045089604No Hit
GTGGTAAGCAGTGGTATCAACGCAG164870.7723717015975872No Hit
CATGTACTCTGCGTTGATACCACTG153500.7191063031190006No Hit
GGTAAGCAGTGGTATCAACGCAGAG140550.6584390286864855No Hit
GTATCAACGCAGAGTACTTTTTTTT113130.5299836877645117No Hit
CCATGTACTCTGCGTTGATACCACT103720.48590036325408953No Hit
GTATCAACGCAGAGTACATGGGAAG97600.4572298057616577No Hit
ACTCTGCGTTGATACCACTGCTTAC94800.4441125572357085No Hit
GTACATGGGAAGCAGTGGTAAGCAG85440.4002634693061069No Hit
GTACTCTGCGTTGATACCACTGCTT81690.38269572574456784No Hit
GAGTACATGGTAAGCAGTGGTATCA81320.38096237504649594No Hit
GCAGTGGTATCAACGCAGAGTACAT78760.36896946210848525No Hit
GGTATCAACGCAGAGTACATGGGAA76970.36058379251511063No Hit
GCGTTGATACCACTGCTTACCATGT71190.33350604377225834No Hit
GCTTACCATGTACTCTGCGTTGATA70860.3319600823388429No Hit
TATCAACGCAGAGTACTTTTTTTTT70700.3312105252802172No Hit
GTGGTATCAACGCAGAGTACATGGG69050.32348071811314005No Hit
GCTTACCACTGCTTCCCATGTACTC68720.3219347566797246No Hit
GGTATCAACGCAGAGTACTTTTTTT68090.318983375761386No Hit
TATCAACGCAGAGTACATGGGAAGC64260.30104085367053407No Hit
CTGCTTACCATGTACTCTGCGTTGA60530.2835668047413232No Hit
GCGTTGATACCACTGCTTACCACTG60310.2825361637857129No Hit
AAGCAGTGGTATCAACGCAGAGTAC60160.2818334540432513No Hit
GTACATGGGAAGCAGTGGTATCAAC59700.27967847749970254No Hit
GTACATGATAAGCAGTGGTATCAAC58020.271808128384133No Hit
ATCATGTACTCTGCGTTGATACCAC55960.26215758125432753No Hit
GTACTTTTTTTTTTTTTTTTTTTTT53850.2522727975437015No Hit
GTATCAACGCAGAGTACATGGTAAG53430.2503052102648091No Hit
ATGGTAAGCAGTGGTATCAACGCAG52320.24510515817059356No Hit
CAGTGGTATCAACGCAGAGTACATG49120.2301140169980802No Hit
TATCAACGCAGAGTACATGGTAAGC45980.21540395972255147No Hit
ACGCAGAGTACATGGTAAGCAGTGG44690.20936065593738198No Hit
GTACATAAGCAGTGGTATCAACGCA42880.20088129171167915No Hit
GTGATAAGCAGTGGTATCAACGCAG40940.1917929123758429No Hit
ACGCAGAGTACATGGGAAGCAGTGG39250.18387571594410929No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA36470.17085216205048828No Hit
GAGTACATGGGAAGCAGTGGTAAGC35810.1677602391836574No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35110.1644809270521701No Hit
GGTATCAACGCAGAGTACATGGTAA34610.16213856124396492No Hit
TACCACTGCTTACCATGTACTCTGC34420.1612484622368469No Hit
GAGTACTTTTTTTTTTTTTTTTTTT32280.1512231365777286No Hit
GTGGTATCAACGCAGAGTACATGGT30550.14311855088133854No Hit
GCTTCCCATGTACTCTGCGTTGATA27250.12765893654718413No Hit
AAAAAGTACTCTGCGTTGATACCAC27140.127143616069379No Hit
GAGTAAGCAGTGGTATCAACGCAGA26750.12531657073897895No Hit
GCTTATGTACTCTGCGTTGATACCA26550.12437962441569685No Hit
GATAAGCAGTGGTATCAACGCAGAG25490.11941380890230179No Hit
TGGTAAGCAGTGGTATCAACGCAGA25390.11894533574066075No Hit
GTAAGCAGTGGTATCAACGCAGAGT25220.11814893136587098No Hit
GCAGAGTACATGGTAAGCAGTGGTA24650.11547863434451704No Hit
GAGTACATGGGAAGCAGTGGTATCA24630.11538493971218883No Hit
ACTCTGCGTTGATACCACTGCTTAT23270.10901370471387065No Hit
GTACATGGTAAGCAGTGGTAAGCAG23250.10892001008154244No Hit
CTGCTTACCACTGCTTCCCATGTAC21960.10287670629637298No Hit
GAGTACATGATAAGCAGTGGTATCA21740.1018460653407627No Hit
AAAGTACTCTGCGTTGATACCACTG21420.10034695122351137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAAA456.770626E-414.77580913
TGCGATG951.0417461E-611.99868312
CCTACAC1001.9259805E-611.4022223
GTCTAAC951.3518244E-511.0065281
CTGCGAT1106.055401E-610.36249911
ATAAGAC1201.5204223E-610.2936733
GTCTTAG1701.544322E-910.0647931
GTCTTGG1752.5484042E-99.7772271
GTACATA12950.09.6157431
GTCTAAG1201.6888798E-59.5056381
GCTTTAG1002.7395465E-49.5056381
TTATACT2900.09.5018514
TCTGTCG1002.7583094E-49.499188
CGATGAT1106.847868E-59.49873414
CTGCTAA2357.2759576E-129.30339051
GTATTAT2151.05501385E-109.2845751
CTAATAC2054.2200554E-109.2700993
GCTTATG7200.09.2415921
TAATACT3000.09.18512254
CTAAGCT1356.8314603E-69.1499314
Produced by FastQC (version 0.11.5)