FastQCFastQC Report
Wed 25 May 2016
SRR1294537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993863
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA453832.2761343181552594No Hit
GCTTACTCTGCGTTGATACCACTGC250841.258060358209165No Hit
GCGTTGATACCACTGCTTACTCTGC172530.8653051889723616No Hit
CTGCTTACTCTGCGTTGATACCACT156340.7841060293510638No Hit
GTATCAACGCAGAGTAAGCAGTGGT113630.5698987342660955No Hit
TGCTTACTCTGCGTTGATACCACTG102670.5149300628980025No Hit
ACTCTGCGTTGATACCACTGCTTAC100870.5059023613959435No Hit
CCCATGTACTCTGCGTTGATACCAC98810.49557065856580923No Hit
TATCAACGCAGAGTAAGCAGTGGTA88560.44416291390130613No Hit
ACGCAGAGTAAGCAGTGGTATCAAC81600.4092558014266777No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA79450.39847271352144054No Hit
GTGGTATCAACGCAGAGTAAGCAGT74810.3752013052050216No Hit
GGTATCAACGCAGAGTAAGCAGTGG72710.3646689867859527No Hit
GTAAGCAGTGGTATCAACGCAGAGT69740.3497732793075552No Hit
ACCATGTACTCTGCGTTGATACCAC68960.3458612753233296No Hit
GTACATGGTAAGCAGTGGTATCAAC68650.3443065045090861No Hit
CATGTACTCTGCGTTGATACCACTG55030.27599689647683917No Hit
CCACTGCTTACTCTGCGTTGATACC52670.26416057672969506No Hit
AAGCAGTGGTATCAACGCAGAGTAA50890.2552331830221033No Hit
GTGGTAAGCAGTGGTATCAACGCAG44660.22398730504553221No Hit
GATACCACTGCTTACTCTGCGTTGA42360.21245190868179006No Hit
GTTGATACCACTGCTTACTCTGCGT39600.19860943304529952No Hit
TACCACTGCTTACTCTGCGTTGATA38080.19098604066578295No Hit
GGTAAGCAGTGGTATCAACGCAGAG37670.18892973087920284No Hit
GTGGTATCAACGCAGAGTACATGGG37280.18697372888709002No Hit
TGATACCACTGCTTACTCTGCGTTG36180.18145680019138727No Hit
GCAGTGGTATCAACGCAGAGTAAGC35470.1778958734877973No Hit
CAGTGGTATCAACGCAGAGTAAGCA35160.1763411026735538No Hit
ATACCACTGCTTACTCTGCGTTGAT33380.16741370896596205No Hit
CCATGTACTCTGCGTTGATACCACT31610.158536469155604No Hit
GTACTCTGCGTTGATACCACTGCTT26620.1335096744360069No Hit
GTATCAACGCAGAGTACATGGGAAG26570.13325890494983858No Hit
GAGTACATGGTAAGCAGTGGTATCA25700.12889551589051002No Hit
GCTTACCATGTACTCTGCGTTGATA25090.12583612815925668No Hit
ACTGCTTACTCTGCGTTGATACCAC24800.12438166513948049No Hit
GCAGTGGTATCAACGCAGAGTACAT24340.12207458586673207No Hit
GTACATAAGCAGTGGTATCAACGCA23150.11610627209592635No Hit
GCGTTGATACCACTGCTTACCATGT21420.1074296478745029No Hit
GTACATGGGAAGCAGTGGTATCAAC20950.10507241470452083No Hit
GGTATCAACGCAGAGTACATGGGAA20670.10366810558197831No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGC456.777815E-414.7736628
ACGAAAC400.005289562514.2460313
ACTAGAC701.0940402E-412.2108843
AGTTATA658.027031E-411.6919832
CTCGAAT658.042248E-411.68934512
AGCCTCG1803.6379788E-1211.0830262
CCTCGGT1303.2807293E-810.9584874
GACGTAG700.001495506110.85384710
GTCTAGA1502.0881998E-910.7804321
GCGAAAA909.394757E-510.5690511
CTAGACA1355.7123543E-810.5526164
TAGACAG1106.066959E-610.360755
TAGGGTG1201.5294936E-610.2887995
CTAGACT750.002654258410.1305114
CGGTCTC750.002654258410.1305117
TCTACAC2107.2759576E-129.949613
CCTATAC1252.5795289E-69.8772483
ACATAAG5600.09.8365453
CTAGAAC1451.6239028E-79.8248493
CTAGGAC1554.0814484E-89.803723
Produced by FastQC (version 0.11.5)