FastQCFastQC Report
Wed 25 May 2016
SRR1294537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993863
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA473302.3737839560691985No Hit
GCTTACTCTGCGTTGATACCACTGC253401.2708997559009823No Hit
GCGTTGATACCACTGCTTACTCTGC175110.8782448944586463No Hit
CTGCTTACTCTGCGTTGATACCACT159220.7985503517543582No Hit
GTATCAACGCAGAGTAAGCAGTGGT118360.5936215276576174No Hit
CCCATGTACTCTGCGTTGATACCAC104890.5260642280838753No Hit
ACTCTGCGTTGATACCACTGCTTAC103290.5180396045264896No Hit
TGCTTACTCTGCGTTGATACCACTG101770.510416212146973No Hit
TATCAACGCAGAGTAAGCAGTGGTA94670.47480694511107335No Hit
ACGCAGAGTAAGCAGTGGTATCAAC87700.43984967873921127No Hit
GTGGTATCAACGCAGAGTAAGCAGT78500.3937080932842427No Hit
GGTATCAACGCAGAGTAAGCAGTGG77850.3904480899640547No Hit
GTAAGCAGTGGTATCAACGCAGAGT75780.38006623323668676No Hit
ACCATGTACTCTGCGTTGATACCAC73560.3689320680508139No Hit
GTATCAACGCAGAGTACTTTTTTTT73180.36702621995593476No Hit
GTACATGGTAAGCAGTGGTATCAAC70370.3529329748332759No Hit
CATGTACTCTGCGTTGATACCACTG57790.2898393721133298No Hit
CCACTGCTTACTCTGCGTTGATACC53560.26862427358349095No Hit
AAGCAGTGGTATCAACGCAGAGTAA52610.2638596533462931No Hit
GTGGTAAGCAGTGGTATCAACGCAG50460.2530765654410559No Hit
GGTATCAACGCAGAGTACTTTTTTT49220.24685748218408182No Hit
TATCAACGCAGAGTACTTTTTTTTT44220.22178053356725114No Hit
GATACCACTGCTTACTCTGCGTTGA43850.21992483936960563No Hit
GGTAAGCAGTGGTATCAACGCAGAG41300.20713559557502195No Hit
GTTGATACCACTGCTTACTCTGCGT40700.20412636174100227No Hit
TACCACTGCTTACTCTGCGTTGATA39490.19805774017572922No Hit
GTGGTATCAACGCAGAGTACATGGG38210.19163804132982057No Hit
CAGTGGTATCAACGCAGAGTAAGCA37450.18782634514006227No Hit
GCAGTGGTATCAACGCAGAGTAAGC37390.18752542175666032No Hit
TGATACCACTGCTTACTCTGCGTTG36990.18551926586731385No Hit
ATACCACTGCTTACTCTGCGTTGAT35310.1770934111320587No Hit
CCATGTACTCTGCGTTGATACCACT34790.17448540847590832No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29380.14735215007249744No Hit
GTACTCTGCGTTGATACCACTGCTT28760.14424260844401046No Hit
GTATCAACGCAGAGTACATGGGAAG28630.14359060777997285No Hit
GAGTACATGGTAAGCAGTGGTATCA26560.1332087510526049No Hit
GCTTACCATGTACTCTGCGTTGATA26210.13145336464942678No Hit
GCAGTGGTATCAACGCAGAGTACAT25910.1299487477324169No Hit
ACTGCTTACTCTGCGTTGATACCAC25560.12819336132923875No Hit
GTACATGGGAAGCAGTGGTATCAAC23900.11986781438845097No Hit
GGTATCAACGCAGAGTACATGGGAA22960.11515334804848677No Hit
GCGTTGATACCACTGCTTACCATGT22800.1143508856927482No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22680.11374903892594425No Hit
GTACATAAGCAGTGGTATCAACGCA22570.11319734605637398No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22150.11109088237256019No Hit
AAGCAGTGGTATCAACGCAGAGTAC21440.10752995566897024No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21250.10657703162153068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGC951.0386448E-612.0015363
CGGGACT600.00587104711.0847534
GTGTAGA2050.010.6649851
GTGTTAC1002.3833474E-510.4563221
ACTGACC1659.313226E-1010.3628848
GCTTATG4550.010.23695851
CACGGCT750.002650831610.1320915
CTTCTAT1803.8926373E-1010.0338441
CTGTGCG1054.1090683E-59.9516589
ACATAAG5650.09.7534613
GTATAAT2054.0017767E-119.7375951
GCCTAAA1304.2064657E-69.5057481
GTTTAGA1502.62522E-79.5057471
TCCTAAT800.00449391979.5057471
GGCTTAC900.001107669.5057471
GCTCTAG1804.138201E-99.5057471
GCGTAGA800.00449391979.5057471
GTGTAAT1901.0404619E-99.5057461
TTAACCT1201.6980272E-59.5012174
TAGGACC900.00111250029.5012174
Produced by FastQC (version 0.11.5)