FastQCFastQC Report
Wed 25 May 2016
SRR1294536_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294536_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426102
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC73130.5127964198914243No Hit
ACCATGTACTCTGCGTTGATACCAC61600.4319466630016647No Hit
GTACATGGTAAGCAGTGGTATCAAC59410.4165901176774172No Hit
ATTCCATTCCATTCCATTCCATTCC48550.34043848195991594No Hit
GTGGTAAGCAGTGGTATCAACGCAG41520.2911432702569662No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39830.2792927855090309No Hit
GAATGGAATGGAATGGAATGGAATG39260.275295876452035No Hit
CATGTACTCTGCGTTGATACCACTG38860.27249102799098524No Hit
GGTAAGCAGTGGTATCAACGCAGAG32960.2311195131905011No Hit
GTGGTATCAACGCAGAGTACATGGG31760.2227049678073518No Hit
CCATGTACTCTGCGTTGATACCACT28970.20314114979152967No Hit
ACTCTGCGTTGATACCACTGCTTAC24840.17418108943119076No Hit
GTATCAACGCAGAGTACATGGGAAG23430.1642939986059903No Hit
GCTTACCATGTACTCTGCGTTGATA21720.15230327143500255No Hit
GAGTACATGGTAAGCAGTGGTATCA21710.1522331502234763No Hit
GGTATCAACGCAGAGTACATGGGAA21560.15118133205058265No Hit
GCAGTGGTATCAACGCAGAGTACAT19280.13519369582259894No Hit
GTACTCTGCGTTGATACCACTGCTT18500.12972424132355187No Hit
GCGTTGATACCACTGCTTACCATGT17870.12530660499739849No Hit
GTACATGGGAAGCAGTGGTAAGCAG17070.11969690807529895No Hit
GCTTACCACTGCTTCCCATGTACTC16870.11829448384477408No Hit
CTGCTTACCATGTACTCTGCGTTGA15620.10952933240399355No Hit
GTACATAAGCAGTGGTATCAACGCA15050.10553242334699763No Hit
GCGTTGATACCACTGCTTACCACTG14910.1045507263856302No Hit
GTATCAACGCAGAGTACATGGTAAG14510.10174587792458044No Hit
TATCAACGCAGAGTACATGGGAAGC14380.10083430217473927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGT400.005289820514.2455296
GTCTTAG907.3405117E-611.6278561
GTCCAGG1354.618414E-911.27549651
ACTACAC1502.1391315E-910.7640443
GTGTAGG1605.293259E-1010.7029131
TAACCCT856.604888E-410.0567254
CTAAGGC856.607018E-410.0563733
ACAATAC856.607018E-410.0563733
TAGACTG1901.0004442E-109.9968625
AATCCGA951.6494031E-49.99651211
GTCTATC1054.039085E-59.9667331
GACTTTG1252.5787667E-69.87697
CTACACT1554.0723535E-89.8044064
CAAGACT2054.1836756E-119.7296774
GTTGTAG1304.1588173E-69.51370051
TCGAGTG900.00110625049.50668719
TCTAGGC1304.24901E-69.4976853
CTTGGAC800.0045241469.4976853
GTTGTAC900.00111587839.4976853
CCAAGAC2201.6370905E-119.4976853
Produced by FastQC (version 0.11.5)