FastQCFastQC Report
Wed 25 May 2016
SRR1294536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426102
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC78100.5476466620199677No Hit
ACCATGTACTCTGCGTTGATACCAC62430.43776672355834295No Hit
GTACATGGTAAGCAGTGGTATCAAC60530.4244436933683565No Hit
GTATCAACGCAGAGTACTTTTTTTT54590.3827916937217674No Hit
ATTCCATTCCATTCCATTCCATTCC50630.35502369395737476No Hit
GTGGTAAGCAGTGGTATCAACGCAG46320.32480145178956343No Hit
GAATGGAATGGAATGGAATGGAATG39700.27838120975918973No Hit
CATGTACTCTGCGTTGATACCACTG39260.275295876452035No Hit
GGTATCAACGCAGAGTACTTTTTTT37760.26477769472309837No Hit
GGTAAGCAGTGGTATCAACGCAGAG36670.2571344826667377No Hit
TATCAACGCAGAGTACTTTTTTTTT32690.22922624047929252No Hit
GTGGTATCAACGCAGAGTACATGGG32030.2245982405185604No Hit
CCATGTACTCTGCGTTGATACCACT29810.20903133155973416No Hit
ACTCTGCGTTGATACCACTGCTTAC25990.18224502875670884No Hit
GTATCAACGCAGAGTACATGGGAAG24440.17137624097014098No Hit
GAGTACATGGTAAGCAGTGGTATCA24400.17109575612403602No Hit
GGTATCAACGCAGAGTACATGGGAA23930.16780005918230254No Hit
GCTTACCATGTACTCTGCGTTGATA22930.1607879380296781No Hit
GCAGTGGTATCAACGCAGAGTACAT21250.14900757449326907No Hit
GCGTTGATACCACTGCTTACCATGT18680.1309864231310243No Hit
GTACTCTGCGTTGATACCACTGCTT18620.13056569586186684No Hit
GTACATGGGAAGCAGTGGTAAGCAG18300.128321817093027No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18270.12811145345844827No Hit
GCTTACCACTGCTTCCCATGTACTC18000.12621818074723967No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17240.12088896867124511No Hit
GTACATAAGCAGTGGTATCAACGCA15690.11002018088467726No Hit
CTGCTTACCATGTACTCTGCGTTGA15490.10861775665415238No Hit
AAGCAGTGGTATCAACGCAGAGTAC15330.10749581726973245No Hit
TATCAACGCAGAGTACATGGGAAGC15110.1059531506161551No Hit
GTATCAACGCAGAGTACATGGTAAG15030.10539218092394513No Hit
ATGGTAAGCAGTGGTATCAACGCAG14880.10434036275105148No Hit
GTACATGATAAGCAGTGGTATCAAC14630.10258733246289535No Hit
GCGTTGATACCACTGCTTACCACTG14440.10125502944389672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGAC550.003064417712.0929173
GAACGAA951.0414406E-611.99820616
GCTTTAG657.992695E-411.6975331
GTAAGCC658.011535E-411.694253
CTACCCT752.0703892E-411.4018954
TCTACAC1803.6379788E-1211.0851753
TACACCA1900.011.0018275
CGAAATG909.530709E-510.55360819
GGAACGA1451.4439138E-810.48192715
GTCTAAA1002.3861965E-510.454671
CTTACAC2350.010.1080623
GTATAAA2650.010.0422211
GTATAGA1803.9108272E-1010.0322581
ACGAAAT951.6466703E-49.99815518
GTATTGG1451.6052581E-79.8319781
CTACACT1651.0180884E-89.7895054
TAGTACA1856.4574124E-109.7583774
TAGACTG1304.2268985E-69.5015785
GAACTGT1304.2268985E-69.5015786
CTAAGAC1106.821207E-59.5015783
Produced by FastQC (version 0.11.5)