FastQCFastQC Report
Wed 25 May 2016
SRR1294535_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294535_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609891
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG15950.2615221408415602No Hit
CCCATGTACTCTGCGTTGATACCAC13990.22938525080711142No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA13650.22381048416848254No Hit
GTACATGGTAAGCAGTGGTATCAAC11890.1949528686273449No Hit
GTATCAACGCAGAGTACTTTTTTTT10970.1798682059581138No Hit
ACCATGTACTCTGCGTTGATACCAC10950.1795402785087827No Hit
GTGGTAAGCAGTGGTATCAACGCAG8890.14576375122767837No Hit
ATTCCATTCCATTCCATTCCATTCC8380.13740160126973508No Hit
GGTATCAACGCAGAGTACTTTTTTT7760.12723585034047066No Hit
CATGTACTCTGCGTTGATACCACTG7600.12461243074582179No Hit
GAATGGAATGGAATGGAATGGAATG6550.10739623965593853No Hit
TATCAACGCAGAGTACTTTTTTTTT6290.10313318281463409No Hit
GGTAAGCAGTGGTATCAACGCAGAG6130.10050976321998521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGGG400.005281490314.24704712
CGCCTCC500.001500065713.29724411
ACTGTCC500.001500993313.2961548
GCTACAC701.0925848E-412.2097523
GTATTAG657.909833E-411.7100551
CTAGACA658.0361444E-411.6879684
GACAGCA658.0361444E-411.6879687
TAGACAG855.3304582E-511.1723215
TATACCC600.005884961711.079225
AATACCT600.005884961711.079225
ACATAAG1252.2410495E-710.636053
CCATGCA909.520289E-510.5525039
TAGGACA909.528211E-510.5516374
CCTCTCT1451.4009856E-810.498671
TGTTAGC1054.091345E-59.9527672
GGTGTGC1054.1061274E-59.9495038
GAAGCAG3100.09.8036169
GTCATAG900.00109615229.514421
CTATAAG900.00109615229.514421
TCCAATA800.0045073599.5003692
Produced by FastQC (version 0.11.5)