##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294535_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	609891
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.507716952701383	33.0	31.0	34.0	30.0	34.0
2	31.610543523350895	33.0	31.0	34.0	30.0	34.0
3	31.874633336120716	34.0	31.0	34.0	30.0	34.0
4	35.206889427783	37.0	35.0	37.0	33.0	37.0
5	35.36379779337619	37.0	35.0	37.0	33.0	37.0
6	35.224510609272805	37.0	35.0	37.0	33.0	37.0
7	35.299302662279	37.0	36.0	37.0	33.0	37.0
8	35.36865275926354	37.0	36.0	37.0	33.0	37.0
9	37.07467071984994	39.0	39.0	39.0	35.0	39.0
10	37.1858807557416	39.0	39.0	39.0	35.0	39.0
11	37.145088220682055	39.0	39.0	39.0	35.0	39.0
12	37.14392735751142	39.0	39.0	39.0	35.0	39.0
13	37.04593279782781	39.0	39.0	39.0	34.0	39.0
14	38.51468377136242	41.0	39.0	41.0	34.0	41.0
15	38.545667996412476	41.0	39.0	41.0	35.0	41.0
16	38.54167875899136	41.0	39.0	41.0	34.0	41.0
17	38.468509946859356	41.0	39.0	41.0	34.0	41.0
18	38.45537317323915	41.0	39.0	41.0	34.0	41.0
19	38.530975207045195	41.0	39.0	41.0	34.0	41.0
20	38.564379536671304	41.0	40.0	41.0	34.0	41.0
21	38.55778491566526	41.0	39.0	41.0	34.0	41.0
22	38.50391791320089	41.0	39.0	41.0	34.0	41.0
23	38.4807203254352	41.0	39.0	41.0	34.0	41.0
24	38.58704424233183	41.0	39.0	41.0	34.0	41.0
25	37.84940095853194	41.0	38.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.1724238098267108
1101	2	-0.2073954077839808
1101	3	-0.12432731540593522
1101	4	-0.13088303184386518
1101	5	-0.12955614859475872
1101	6	0.06510351298807393
1101	7	-0.06894179788497468
1101	8	-0.07682193382671443
1101	9	-0.1542738965328283
1101	10	-0.1712527479859247
1101	11	-0.017313921045840175
1101	12	-0.07553734684538682
1101	13	-0.22939708845959217
1101	14	-0.21835752512102147
1101	15	0.024729659983805163
1101	16	-0.04187402097490178
1101	17	-0.21123264171185951
1101	18	-0.10897699659162186
1101	19	-0.16618406134094243
1101	20	-0.19906770692214337
1101	21	-0.22985219344815278
1101	22	-0.10493872575483465
1101	23	-0.04714666016509739
1101	24	-0.17283099545071678
1101	25	-0.24309738638100242
1102	1	-0.3060967879405361
1102	2	-0.23796935747656178
1102	3	-0.3061471116190688
1102	4	-0.14565600127583167
1102	5	-0.37082101627535735
1102	6	-0.2696511456176225
1102	7	-0.28315478752199397
1102	8	-0.2757982639267169
1102	9	-0.2734540924271016
1102	10	-0.15156663658980563
1102	11	-0.22430715708975413
1102	12	-0.3042036557142964
1102	13	-0.29296604213793387
1102	14	-0.354970495815806
1102	15	-0.36277122793341476
1102	16	-0.3443519019703203
1102	17	-0.23434019413843998
1102	18	-0.354107263913896
1102	19	-0.22666700938397355
1102	20	-0.27399805474891537
1102	21	-0.3173889926841511
1102	22	-0.2870623869729769
1102	23	-0.3576375008056587
1102	24	-0.222280092142924
1102	25	-0.2959955481950374
1103	1	-0.13147177593969417
1103	2	-0.28002541315229834
1103	3	-0.2227448372808496
1103	4	-0.18271766415329438
1103	5	0.0479470743549939
1103	6	-0.03398015047719838
1103	7	-0.2149757084103996
1103	8	-0.2233397766251386
1103	9	-0.09713364238022137
1103	10	0.009017901423753472
1103	11	-0.04878029461021072
1103	12	-0.10597120094470114
1103	13	-0.1254016103854454
1103	14	-0.0039402618657788935
1103	15	-0.09769051078355062
1103	16	-0.10334027698487347
1103	17	-0.13350861241730172
1103	18	-0.05501462967299631
1103	19	-0.1616827075061238
1103	20	-0.11823632303258336
1103	21	-0.19050281785793288
1103	22	-0.16802527322134608
1103	23	-0.21477335441785783
1103	24	-0.07208981496944489
1103	25	-0.35954383447485583
1104	1	-0.18022932754718468
1104	2	-0.20621384753904337
1104	3	-0.14423236128286732
1104	4	-0.08373087498417675
1104	5	-0.10327497042991496
1104	6	0.02426406858482011
1104	7	-0.07198985255482171
1104	8	-0.1548634513754692
1104	9	-0.01898378495295816
1104	10	-0.1433899767929674
1104	11	-0.013205291655722817
1104	12	0.012072759738956051
1104	13	0.002105785922310588
1104	14	-0.09142224457119852
1104	15	-0.009500462289629752
1104	16	-0.08621033731692762
1104	17	-0.15203852238891358
1104	18	-0.3125556313285145
1104	19	-0.18642864723871355
1104	20	-0.254599087054757
1104	21	-0.32455653995069156
1104	22	-0.4172755416499925
1104	23	-0.23806376946966168
1104	24	-0.07834422775462713
1104	25	-0.5070770686183721
1105	1	-0.20842593365879836
1105	2	-0.22719736836629068
1105	3	-0.1652209890807228
1105	4	-0.08816270407633908
1105	5	-0.030603800096102418
1105	6	0.0368537725235214
1105	7	0.1263873564653153
1105	8	-0.11131835580362548
1105	9	-0.01135703504947827
1105	10	0.09009152678088839
1105	11	0.04028511847486982
1105	12	0.04968355546625958
1105	13	0.1603994040475598
1105	14	0.11327330848308037
1105	15	-0.013469599844860625
1105	16	0.02424015877419805
1105	17	-0.007180322534765082
1105	18	0.02325730646654023
1105	19	-0.10302977444926853
1105	20	-0.10563692562137561
1105	21	-0.14107125721858438
1105	22	0.0052251580049826885
1105	23	0.00351228251521718
1105	24	-0.03935250717537997
1105	25	-0.11947332051050807
1106	1	-0.36233654993081643
1106	2	-0.4093606472010798
1106	3	-0.5022819450392539
1106	4	-0.4096975159510876
1106	5	-0.4074536271780502
1106	6	-0.5091878737313777
1106	7	-0.5509302561607257
1106	8	-0.5530056774395362
1106	9	-0.42022640739551065
1106	10	-0.47736631727809
1106	11	-0.5205030292903245
1106	12	-0.536635801974839
1106	13	-0.5506825689067654
1106	14	-0.7737681564111156
1106	15	-0.6357682112889336
1106	16	-0.5939382292835091
1106	17	-0.6873496759108875
1106	18	-0.6762290466615184
1106	19	-0.6287381756191479
1106	20	-0.64964399660429
1106	21	-0.5849693033175569
1106	22	-0.6337263269639664
1106	23	-0.5680157543906503
1106	24	-0.7292621812055629
1106	25	-0.5280571018603695
1107	1	0.1296842679314807
1107	2	0.06478279526525199
1107	3	0.20143430572117538
1107	4	0.09695447065921314
1107	5	0.12452042382550843
1107	6	0.34893130945982165
1107	7	0.26153443372480467
1107	8	0.3431229396846902
1107	9	0.2944327512348224
1107	10	0.25874073420986576
1107	11	0.17651320793993364
1107	12	0.18480083295963112
1107	13	0.37100359944360406
1107	14	0.28476787978775064
1107	15	0.2928617201203636
1107	16	0.22759890068972766
1107	17	0.35361263896599127
1107	18	0.38583722635654993
1107	19	0.38507395339871664
1107	20	0.3681177010492078
1107	21	0.39156154184261993
1107	22	0.4200183784554312
1107	23	0.35496455870355703
1107	24	0.21561737450927865
1107	25	0.3730952343652518
1108	1	0.08383475880555835
1108	2	0.07728328336487067
1108	3	-0.07255296464080274
1108	4	0.20817130814361917
1108	5	0.1852821469507404
1108	6	0.15599837537484262
1108	7	0.1885054827382291
1108	8	0.04041280509250811
1108	9	0.17397901383036185
1108	10	0.13897136384809983
1108	11	0.09120161541988381
1108	12	-0.05214943842375419
1108	13	0.07791467899664895
1108	14	0.11429163842197454
1108	15	0.05374017612255244
1108	16	0.22281449685974053
1108	17	0.12622089946115977
1108	18	0.08435710280055275
1108	19	0.044628066691267065
1108	20	0.1112673513643685
1108	21	0.1002729383007619
1108	22	0.10603982195610229
1108	23	0.05646543933802661
1108	24	0.06044382683684546
1108	25	0.18195234140395655
1109	1	0.3308537141089012
1109	2	0.3747687267398092
1109	3	0.40873046911704947
1109	4	0.31546567732836195
1109	5	0.2739022042476478
1109	6	0.34542464575201137
1109	7	0.2956181581994315
1109	8	0.35164570264259964
1109	9	0.35267267821247117
1109	10	0.22259906918313277
1109	11	0.38156720009337874
1109	12	0.46067687082741315
1109	13	0.3249040562519738
1109	14	0.39449505088363424
1109	15	0.3504840555793294
1109	16	0.33745901097691444
1109	17	0.3661685885705239
1109	18	0.34832639233584217
1109	19	0.31562002991307736
1109	20	0.20039868634843572
1109	21	0.3198434650435402
1109	22	0.4262623333859068
1109	23	0.3628707769080606
1109	24	0.4073795410621912
1109	25	0.443523696332079
1110	1	0.24754564664469925
1110	2	0.01468894434391288
1110	3	0.22801381356736172
1110	4	0.11865394125996431
1110	5	0.1235977144305167
1110	6	0.30228497891497597
1110	7	0.2034764210049005
1110	8	0.12048032525714092
1110	9	0.20008992888165977
1110	10	0.043796844631636134
1110	11	0.16066915004097382
1110	12	0.13172608296542165
1110	13	0.04986702370664631
1110	14	0.21570582020768825
1110	15	0.11019264626358449
1110	16	0.18665209130505644
1110	17	0.2933184252808232
1110	18	0.19704519620628247
1110	19	0.2972185364836406
1110	20	0.4069146818092406
1110	21	0.3235380569925397
1110	22	0.38223258607347077
1110	23	0.2546209653192548
1110	24	0.3084550008288218
1110	25	0.3192589331238054
1111	1	0.13379359227611332
1111	2	0.09623072020674783
1111	3	0.12946852862088676
1111	4	0.031228897951649515
1111	5	0.013299220227168007
1111	6	0.27913613158232664
1111	7	-0.05128305129998978
1111	8	0.03055450462017717
1111	9	0.10299491711068498
1111	10	0.02135425590512341
1111	11	0.11403387264569176
1111	12	0.10797536116024276
1111	13	0.10475308953527929
1111	14	0.1539415062557481
1111	15	0.2602748210070658
1111	16	0.25246739801673357
1111	17	0.174507071840587
1111	18	0.2369332608800221
1111	19	0.2108173460603311
1111	20	0.14972772316312444
1111	21	0.23809631222285077
1111	22	0.09677085285266429
1111	23	0.20136728966519257
1111	24	0.23495524513211308
1111	25	0.23907415099792217
1112	1	-0.12320364799807848
1112	2	0.11332321553301838
1112	3	0.15820528638817777
1112	4	0.07237551951312327
1112	5	-0.11799696066841392
1112	6	0.12355881474799446
1112	7	0.16901873807061207
1112	8	-0.06879562446377463
1112	9	0.07837104103127501
1112	10	0.04645885841558339
1112	11	-0.05409574544386686
1112	12	-0.12075249426573009
1112	13	0.009373404678157726
1112	14	0.03427178311856949
1112	15	-0.019040588945749448
1112	16	0.14119258274031665
1112	17	0.04707450707605432
1112	18	-0.06614694921523068
1112	19	0.0971700568232663
1112	20	0.06698491180374333
1112	21	0.008531636713186685
1112	22	0.1791486682181045
1112	23	0.15791496170293584
1112	24	0.12945804936963157
1112	25	0.094785002951987
1113	1	0.27690497326964447
1113	2	0.15894529619292186
1113	3	0.264437115864272
1113	4	0.31602932768750236
1113	5	0.31167019437295096
1113	6	0.2097910116533228
1113	7	0.21541242012491324
1113	8	0.22775421501557958
1113	9	0.08698725660200779
1113	10	0.20274832835298184
1113	11	0.26505099201416726
1113	12	0.1622598743209025
1113	13	0.19011988309858197
1113	14	0.41044009326558495
1113	15	0.2091455966335687
1113	16	0.3932455935932566
1113	17	0.30910781453624026
1113	18	0.41397742558095985
1113	19	0.3040361610170095
1113	20	0.39993091348473087
1113	21	0.2626881799389409
1113	22	0.36852973367103203
1113	23	0.3231203316812099
1113	24	0.27240065233019095
1113	25	0.31648032144423865
1114	1	0.7908546847235449
1114	2	0.7523026375888549
1114	3	0.5877178753920695
1114	4	0.5923711151968902
1114	5	0.5672559204884564
1114	6	0.7252043162283357
1114	7	0.5479330741199391
1114	8	0.5370743503337536
1114	9	0.6197300172724312
1114	10	0.577202209121495
1114	11	0.5188739871162653
1114	12	0.4983573444873528
1114	13	0.48242510645701486
1114	14	0.5534562505633289
1114	15	0.6117492517648131
1114	16	0.7508001092273844
1114	17	0.7166247774521324
1114	18	0.7683877837119297
1114	19	0.8893976620986592
1114	20	0.8705377268958614
1114	21	0.8199987633862165
1114	22	0.7311991221073626
1114	23	0.7947105769614424
1114	24	0.7725065702534266
1114	25	0.8359262102240024
1115	1	0.37555427474248404
1115	2	0.419701859249173
1115	3	0.41558826310512487
1115	4	0.2296564994760928
1115	5	0.29149891230964897
1115	6	0.532916954883099
1115	7	0.5224101921820008
1115	8	0.25749223965967616
1115	9	0.39180135813943195
1115	10	0.1651858098790271
1115	11	0.29871623805797043
1115	12	0.22692490811766675
1115	13	0.18339699049688818
1115	14	0.17071291535698663
1115	15	0.29115238593475823
1115	16	0.3830824758794833
1115	17	0.38403232360101214
1115	18	0.48391087376208475
1115	19	0.5212221818797218
1115	20	0.5772118856022388
1115	21	0.6219924783973525
1115	22	0.5334508962639859
1115	23	0.5999008955812428
1115	24	0.5362575848004738
1115	25	0.6777026560836106
1116	1	0.601900163068418
1116	2	0.5995375807685761
1116	3	0.5075869048383232
1116	4	0.2693738363756282
1116	5	0.5415396752726451
1116	6	0.5429753094954464
1116	7	0.3489020792304487
1116	8	0.41279538895383894
1116	9	0.4264850300671341
1116	10	0.22532651315606955
1116	11	0.47276586266473686
1116	12	0.36251374220717025
1116	13	0.25134024845240077
1116	14	0.4689182978631692
1116	15	0.39376734870957364
1116	16	0.4188192533466122
1116	17	0.6315809362391818
1116	18	0.4663207202988744
1116	19	0.3825607927078636
1116	20	0.4740509235065602
1116	21	0.46344175506044394
1116	22	0.6089304407360032
1116	23	0.6339368477666625
1116	24	0.5947228929011601
1116	25	0.6259942765647182
1201	1	-0.3406156247788026
1201	2	-0.2678681781197163
1201	3	-0.2631939892497712
1201	4	-0.09622884319711744
1201	5	-0.14118316140600484
1201	6	-0.10554135559284816
1201	7	-0.23780647237475705
1201	8	-0.25146551051732047
1201	9	-0.07875598693439656
1201	10	-0.16342835279052537
1201	11	-0.17479381215794376
1201	12	-0.19734799263674319
1201	13	-0.21276015577630147
1201	14	-0.33155172391833077
1201	15	-0.3258070896816889
1201	16	-0.36235894123143453
1201	17	-0.283709226199953
1201	18	-0.1996784622719403
1201	19	-0.15865956925377134
1201	20	-0.1679200776946672
1201	21	-0.25853138940022546
1201	22	-0.3549195011966191
1201	23	-0.2046919147751396
1201	24	-0.1418717668937859
1201	25	-0.2912933538376663
1202	1	-0.6259094478300611
1202	2	-0.491167147986193
1202	3	-0.46198972694711316
1202	4	-0.34505578538790616
1202	5	-0.31227624325423164
1202	6	-0.23834344205626223
1202	7	-0.5518719941061789
1202	8	-0.4679938757871085
1202	9	-0.43017251684062785
1202	10	-0.40213502284731106
1202	11	-0.45266597389738195
1202	12	-0.41353349371927095
1202	13	-0.2900895134260253
1202	14	-0.5217874141540193
1202	15	-0.4457556215295355
1202	16	-0.4755156802462963
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2209	22	-0.27167949809015113
2209	23	-0.29550392862343955
2209	24	-0.17908906827412352
2209	25	-0.19864505074469463
2210	1	-0.07918947329053694
2210	2	-0.1029008473138795
2210	3	-0.04330611315071664
2210	4	0.03769871992299301
2210	5	0.06605649334917985
2210	6	-2.9655881411054708
2210	7	-1.0277919527329047
2210	8	-0.33928917753353716
2210	9	-0.3682816786339984
2210	10	-0.17354370327854696
2210	11	-0.052118971470676456
2210	12	-0.10188134748065636
2210	13	-0.17785655006524337
2210	14	0.0932163651354756
2210	15	-0.1561168420411292
2210	16	-0.10921301883085732
2210	17	-0.008211121819854839
2210	18	-0.05221133312779358
2210	19	-0.04287349197701218
2210	20	-0.11379811270313667
2210	21	0.052729399500314855
2210	22	0.1114473617540952
2210	23	-0.06786848975296067
2210	24	0.055732492201158834
2210	25	-0.11587591413451293
2211	1	-0.03041554511461797
2211	2	0.08400786966128493
2211	3	0.05662269558207811
2211	4	-0.13179977715025615
2211	5	-0.041988119844937444
2211	6	0.004709280188627929
2211	7	-0.13416752621811412
2211	8	0.10490477911888973
2211	9	0.13642485514230174
2211	10	0.03049046852970605
2211	11	0.008765382270816247
2211	12	-0.024045562003678356
2211	13	-0.16616536196209353
2211	14	0.008174441389229514
2211	15	-0.1656011008609397
2211	16	-0.10751233855874887
2211	17	-0.17650727749143158
2211	18	-0.057463666619327114
2211	19	-0.2307709765522361
2211	20	0.0035330057907785317
2211	21	0.10646298595815296
2211	22	0.024451633230128778
2211	23	-0.10794917318912667
2211	24	-0.07175524708060266
2211	25	0.04360043454449425
2212	1	0.014488973698011165
2212	2	-0.02384523393770621
2212	3	-0.006614407643677822
2212	4	-0.060703922730517945
2212	5	-0.020533397596111058
2212	6	0.03826949951174896
2212	7	-0.06965357543076323
2212	8	-0.055283113910746806
2212	9	0.01586466282941501
2212	10	-0.02354596582436841
2212	11	-0.00876180598807963
2212	12	-0.05491089632902657
2212	13	-0.14961545318303138
2212	14	-0.07767145784293916
2212	15	-0.0897133848711249
2212	16	0.03048787261219843
2212	17	0.06775611651671909
2212	18	0.04612795824286309
2212	19	-0.007546101597355914
2212	20	-0.29955991187956954
2212	21	0.0063630480816954105
2212	22	-0.08467463471632897
2212	23	0.02000200212449954
2212	24	0.00757872958746475
2212	25	-0.14051730062259082
2213	1	-0.014683670969610318
2213	2	-0.05732928535575965
2213	3	-0.13008971548053694
2213	4	-0.024966346103418857
2213	5	-0.040117087194147416
2213	6	0.28241105857439663
2213	7	0.147015748125213
2213	8	-0.06574642710003076
2213	9	-0.043118279941189996
2213	10	-0.03256731272232827
2213	11	0.021992895498328835
2213	12	-0.015758009256479966
2213	13	-0.11814822228969035
2213	14	0.1045742416194031
2213	15	0.11679454473580364
2213	16	-0.09568538555532058
2213	17	-0.11755708242632323
2213	18	0.05953558786282542
2213	19	0.07725455924347813
2213	20	-0.055456998479670006
2213	21	0.028415229387825036
2213	22	-0.001171018931508172
2213	23	0.09882707736277752
2213	24	-0.019891090565280933
2213	25	0.005597119523393701
2214	1	0.31543224279921134
2214	2	0.30341891816137334
2214	3	0.22245665604536313
2214	4	0.10769533735449244
2214	5	0.06552092000382004
2214	6	0.2858776385765651
2214	7	0.1781162669085461
2214	8	0.2109588774895812
2214	9	0.32260229926655626
2214	10	0.2917962448553979
2214	11	0.3508858768774985
2214	12	0.22311583371225652
2214	13	0.2649329414219608
2214	14	0.19476670902993476
2214	15	0.23119288848091202
2214	16	0.2914353067977373
2214	17	0.28907154815421165
2214	18	0.1360398789592523
2214	19	0.2513310406647733
2214	20	0.37124529692591324
2214	21	0.3203638565980711
2214	22	0.4364558181753395
2214	23	0.34086417458349416
2214	24	0.22977127898658978
2214	25	0.10564285912064264
2215	1	0.628017854084856
2215	2	0.5505138518991401
2215	3	0.4995399141490928
2215	4	0.3955129567288225
2215	5	0.34659315245726674
2215	6	0.7377487492062045
2215	7	0.4650985367962406
2215	8	0.43207492366249767
2215	9	0.3566638551350678
2215	10	0.5327921644018545
2215	11	0.3280931133632521
2215	12	0.4167038643818657
2215	13	0.36442258381052994
2215	14	0.3638335008786626
2215	15	0.3390167669580251
2215	16	0.5339442440743056
2215	17	0.3410485820616955
2215	18	0.444067178276768
2215	19	0.5870584808121038
2215	20	0.5305996464005105
2215	21	0.5447550986688654
2215	22	0.5249727719181649
2215	23	0.5835765327004623
2215	24	0.5639869050774209
2215	25	0.41736182540574873
2216	1	0.6913320686595732
2216	2	0.704242009828981
2216	3	0.4989841362823917
2216	4	0.415476497700773
2216	5	0.41197486069611955
2216	6	0.5451828339512161
2216	7	0.6118905869496345
2216	8	0.5240895072737217
2216	9	0.47015369551566266
2216	10	0.2747556164493403
2216	11	0.3557041577810196
2216	12	0.26319801759870387
2216	13	0.3593316585515396
2216	14	0.39809164858218793
2216	15	0.46077658199514815
2216	16	0.5115352804888715
2216	17	0.6611766332821816
2216	18	0.5573124763960209
2216	19	0.5496435148257106
2216	20	0.5169275108796327
2216	21	0.4396908507474748
2216	22	0.5697240057450301
2216	23	0.6422222131948843
2216	24	0.5787483652170309
2216	25	0.6858247661375927
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	1.0
6	4.0
7	12.0
8	51.0
9	248.0
10	447.0
11	825.0
12	1208.0
13	1813.0
14	2416.0
15	2939.0
16	3265.0
17	3368.0
18	3148.0
19	2973.0
20	2563.0
21	2349.0
22	2088.0
23	1957.0
24	1763.0
25	1866.0
26	1964.0
27	2029.0
28	2083.0
29	2483.0
30	2957.0
31	3887.0
32	5113.0
33	7270.0
34	10430.0
35	16826.0
36	30110.0
37	72489.0
38	416945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73827877386532	21.30069071071275	12.601611329889087	22.359419185532843
2	14.693295058437563	22.09981546032397	40.65843756407628	22.54845191716219
3	18.105673272749335	28.52968473801411	29.59159913551379	23.773042853722767
4	11.614704864726592	18.736256618550144	38.496554008517	31.152484508206264
5	11.977345917477225	39.50904130074049	34.9480543330688	13.56555844871349
6	29.09628142944751	39.57691790547963	17.97680771589452	13.349992949178333
7	23.894535234178484	34.59843170436545	23.921919735534587	17.58511332592148
8	23.321215818030666	37.94618773786158	21.276651564864782	17.455944879242974
9	23.48734548769969	18.175467595462283	22.282075753252588	36.05511116358544
10	13.771826694935404	31.558994590570638	33.868209313269745	20.800969401224215
11	30.580137176100596	27.135649553267648	22.882960878740327	19.401252391891425
12	20.552522968499172	29.331466228968427	31.677753965273826	18.43825683725857
13	26.02860127280747	25.227726279161672	26.52267096073843	22.221001487292423
14	20.286497149294334	24.865007567447023	28.416542455591465	26.43195282766718
15	23.165864249357192	30.813808574277168	24.765014168022248	21.25531300834339
16	20.861544516139613	32.27545155656858	26.07803750194725	20.78496642534456
17	19.408408807290105	31.099533791506577	28.535305085329437	20.956752315873885
18	19.564429562117407	30.746563335798538	29.415517012369204	20.27349008971485
19	20.1051502638596	29.893373827882858	29.793668025595526	20.207807882662014
20	22.242230126984598	28.941106240334296	28.916834355868463	19.899829276812643
21	22.673073296325754	29.497117836614546	27.257967972318113	20.571840894741587
22	21.314166694000722	28.959228523633023	28.99760553678617	20.72899924558008
23	20.83535035453079	29.512944763430077	28.32783883084426	21.32386605119487
24	20.709313844478412	29.525057567785655	28.909361079584595	20.856267508151337
25	21.916332073689183	28.6949561748806	27.624586679263107	21.76412507216711
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1714.0
1	1714.0
2	1633.0
3	1552.0
4	1552.0
5	1552.0
6	1909.0
7	2266.0
8	2266.0
9	2266.0
10	3048.0
11	3830.0
12	3830.0
13	3830.0
14	5735.5
15	7641.0
16	7641.0
17	7641.0
18	11309.0
19	14977.0
20	14977.0
21	14977.0
22	20240.5
23	25504.0
24	25504.0
25	25504.0
26	31677.5
27	37851.0
28	37851.0
29	37851.0
30	44085.5
31	50320.0
32	50320.0
33	50320.0
34	55548.0
35	60776.0
36	60776.0
37	60776.0
38	65208.5
39	69641.0
40	69641.0
41	69641.0
42	71434.0
43	73227.0
44	73227.0
45	73227.0
46	73619.5
47	74012.0
48	74012.0
49	74012.0
50	69685.5
51	65359.0
52	65359.0
53	65359.0
54	56390.5
55	47422.0
56	47422.0
57	47422.0
58	40644.0
59	33866.0
60	33866.0
61	33866.0
62	27660.0
63	21454.0
64	21454.0
65	21454.0
66	16207.0
67	10960.0
68	10960.0
69	10960.0
70	7865.5
71	4771.0
72	4771.0
73	4771.0
74	3293.0
75	1815.0
76	1815.0
77	1815.0
78	1222.5
79	630.0
80	630.0
81	630.0
82	423.0
83	216.0
84	216.0
85	216.0
86	135.5
87	55.0
88	55.0
89	55.0
90	41.0
91	27.0
92	27.0
93	27.0
94	15.0
95	3.0
96	3.0
97	3.0
98	2.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15625742960627392
2	0.043614350761037625
3	0.008034222508612195
4	0.00852611368260886
5	0.003771165667307765
6	0.005410802913963315
7	0.009345932305936635
8	0.00491891173996665
9	0.011313497001923294
10	0.00491891173996665
11	0.00295134704397999
12	0.00557476663862887
13	0.00950989603060219
14	0.006886476435953311
15	0.010985569552592184
16	0.009181968581271079
17	0.012789170523913288
18	0.011477460726588848
19	0.015904481292558833
20	0.02164321165585326
21	0.01738015481454883
22	0.024758522424498806
23	0.08099807998478417
24	0.028693651816472127
25	0.031972926309783226
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	609891.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.27373822985197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.74044576991797	79.84131526593553
2	3.891405333123369	6.558865487797775
3	0.5478596222541328	1.3851053517765113
4	0.23060938238625428	0.7773725889826812
5	0.12718985796619492	0.5359382397867583
6	0.08045549078963136	0.4068170981975796
7	0.05124151436311797	0.30228197775570226
8	0.04572466209546189	0.3082710563265112
9	0.03397069109612296	0.2576553416029647
>10	0.21073812010644452	3.3432821534695027
>50	0.02125220603991774	1.1976699872371626
>100	0.016377728452950685	2.871334725723546
>500	0.001559783661971757	0.9423235851735396
>1k	0.001169837746478818	1.2717671402342103
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	1595	0.2615221408415602	No Hit
CCCATGTACTCTGCGTTGATACCAC	1399	0.22938525080711142	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1365	0.22381048416848254	No Hit
GTACATGGTAAGCAGTGGTATCAAC	1189	0.1949528686273449	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1097	0.1798682059581138	No Hit
ACCATGTACTCTGCGTTGATACCAC	1095	0.1795402785087827	No Hit
GTGGTAAGCAGTGGTATCAACGCAG	889	0.14576375122767837	No Hit
ATTCCATTCCATTCCATTCCATTCC	838	0.13740160126973508	No Hit
GGTATCAACGCAGAGTACTTTTTTT	776	0.12723585034047066	No Hit
CATGTACTCTGCGTTGATACCACTG	760	0.12461243074582179	No Hit
GAATGGAATGGAATGGAATGGAATG	655	0.10739623965593853	No Hit
TATCAACGCAGAGTACTTTTTTTTT	629	0.10313318281463409	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	613	0.10050976321998521	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.6396372466555498E-4	0.0
10	0.0	0.0	0.0	1.6396372466555498E-4	0.0
11	0.0	0.0	0.0	1.6396372466555498E-4	0.0
12	0.0	1.6396372466555498E-4	0.0	1.6396372466555498E-4	0.0
13	0.0	1.6396372466555498E-4	0.0	1.6396372466555498E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTGGG	40	0.0052814903	14.247047	12
CGCCTCC	50	0.0015000657	13.297244	11
ACTGTCC	50	0.0015009933	13.296154	8
GCTACAC	70	1.0925848E-4	12.209752	3
GTATTAG	65	7.909833E-4	11.710055	1
CTAGACA	65	8.0361444E-4	11.687968	4
GACAGCA	65	8.0361444E-4	11.687968	7
TAGACAG	85	5.3304582E-5	11.172321	5
TATACCC	60	0.0058849617	11.07922	5
AATACCT	60	0.0058849617	11.07922	5
ACATAAG	125	2.2410495E-7	10.63605	3
CCATGCA	90	9.520289E-5	10.552503	9
TAGGACA	90	9.528211E-5	10.551637	4
CCTCTCT	145	1.4009856E-8	10.49867	1
TGTTAGC	105	4.091345E-5	9.952767	2
GGTGTGC	105	4.1061274E-5	9.949503	8
GAAGCAG	310	0.0	9.803616	9
GTCATAG	90	0.0010961522	9.51442	1
CTATAAG	90	0.0010961522	9.51442	1
TCCAATA	80	0.004507359	9.500369	2
>>END_MODULE